Micropropagation of Costus speciosus (Koen.) Sm. Using Nodal Segment Culture

Abstract

Nodal segments of Costus speciosus (Koen.) Sm. containing single axillary buds were cultured on Murashige and Skoog medium (MS medium) supplemented with plant growth regulators for inducing plantlets. For breaking of axillary bud dormancy, nodal segments were cultured on 40-70gl -1 sucrose or 1-13 µM adenine sulphate (AdS) supplemented MS basal medium containing 5 µM 6-benzylaminopurine (BAP) and 1µM α-naphthalene acetic acid (NAA). The nodal segments cultured on 1-13 µM AdS, 5 µM BAP, 1 µM NAA and 50gl -1 sucrose showed simultaneous production of shoots and roots while those cultured on 5 µM BAP, 1 µM NAA and 40-70gl -1 sucrose produced shoots only. The most effective media for breaking axillary bud dormancy was 5 µM BAP, 1 µM NAA, 50 gl -1 sucrose and 10 µM AdS supplemented medium. The propagules from 40-70gl -1 sucrose produced roots in shoot multiplication medium, i.e.,10 µM AdS, 1 µM NAA, 50gl-1 sucrose and 3-11 µM BAP supplemented medium. The best response for shoot multiplication was on 10 µM AdS, 1 µM NAA, 50gl-1 sucrose and 7 µM BAP. The well-rooted shoots were hardened and transferred to the soil where they showed 95% survival rate. Results show that axillary bud can be used for micropropagation of Costus speciosus.

Micropropagation and Microrhizome Induction in Costus pictus D. Don Using In Vitro and Ex Vitro Nodal Segments as Explant

“…It has already been reported that the nodal segments of Costus speciosus show bud-break and shoot multiplication in medium supplemented with BAP, NAA and AdS (Punyarani and Sharma, 2009). In the present investigation, both bud-break and shoot multiplication is possible in media supplemented with BAP and NAA.…”

“…In the present investigation, both bud-break and shoot multiplication is possible in media supplemented with BAP and NAA. The propagules of Costus pictus also produce higher average number of multiple shoot in comparison to propagules of Costus speciosus (Punyarani and Sharma, 2009). However, in both Costus pictus and Costus speciosus, the optimal sucrose concentration for shoot multiplication is 5% sucrose.…”

“…The buds which had mature stem which have produced seeds are used and one mature stem usually contain 15-20 axillary buds in comparison to 4-5 rhizomatous eyes in one rhizome. Shoot multiplication in Costus speciosus has been achieved using shoot tips (Chaturvedi et al, 1984), rhizome thin section (Malabadi et al, 2005) and nodal segment (Punyarani and Sharma, 2009). No report on in vitro regeneration of Costus pictus have been found.…”

Evaluation of some bioactive effect of phenolic compounds in Costus speciosus rhizome extract

“…Costus speciosus have traditional uses such as food and medicine [4]. Recently the juice of rhizome therapeutically implemented as cooling and relief from head-ache, powder of leaves implemented as antipyretic, the extract acquired by boiling stem part of this plant is used against dysentery and fever, and many cures against diarrhea [5] cough, cuts, burning sensation, scabies, arthritis [6], wounds, constipation, leprosy, for abortion, asthma, inflammations, anemia [7] intestinal pains, nose pain , rash, worm infection, to stop vomiting [8] spermatorrhoea [9] used as antivermin and skin diseases [10]. Earlier studies paid attention on antioxidant, cytotoxicity and antimicrobial effects of plant extracts.…”

In vitro acclimatization of <i>Curcuma longa</i> under controlled <i>iso</i>-osmotic conditions

“…Micro-rhizome formation of Thai black ginger plantlets acclimatized under 6% sucrose were evidently observed (Zuraida et al 2015). Similarly, micro-rhizome in painted spiral ginger (Costus speciosus) was produced using 9% sucrose with 1.7 micro-rhizomes per explant (5.12 g micro-rhizome fresh weight) (Punyarani and Sharma 2010). Thus, the mini-rhizome and microrhizome formation in response to sugar classes and concentration in the culture medium depends on plant species.…”