Micropropagation of caari under different nutritive culture media, antioxidants, and levels of agar and pH

Abstract

The caçari (Myrciaria dubia) is a native fruit tree from Amazon with high concentrations of vitamin C. This study aimed to adjust a culture medium that meets the nutritional needs for the in vitro development of caçari, evaluating the effect of different concentrations and nutritive culture media, antioxidant, and levels of agar and pH. Three experiments were carried out in a completely randomized design: 1-woody plant medium (WPM), Murashige and Skoog (MS) and Juan, Antonio, Diva and Silvia medium (JADS) nutritive media with 25, 50, 75 and 100% concentrations; 2-pH (3.7, 4.7, 5.7 and 6.7) and agar concentrations (0, 3.5, 7.0 and 10.5 g.L-1); 3-Antioxidants [(ascorbic acid (AA), citric acid (CA), polyvinylpyrrolidone (PVP)] and concentrations (0, 100, 200, 300 mg.L-1) on the control of phenolic oxidation of stem segments. After collection, the explants were disinfested in a laminar flow chamber, dipping in 70% ethanol for 3 min and 1.5% sodium hypochlorite for 12 min, followed by three washes in distilled and autoclaved water. After disinfestation, the explants were inoculated in 15 × 125 mm test tubes containing 30 mL culture medium, according to each experiment and their respective treatments. After 90 days, the number and length of sprouts (cm) and the oxidation were evaluated. The best results were obtained using the WPM medium at a concentration of 100% with 7 g.L-1 agar, and pH adjusted to 5.7. The use of antioxidants in the tested conditions did not contribute to decrease in oxidation in explants, indicating that there is no need of adding them into the culture medium.

Essential oils from condiment and medicinal plants in the control of contaminants from the micropropa-gation of Myrciaria dubia

“…Subsequently, the explants were immersed in a fungicide solution with 2 ml L -1 Nativo® and 100 mg L -1 of the antioxidant citric acid, as recommended by Araújo et al (2016), remaining under these conditions for 2 h. After this step, in a laminar flow chamber, the stem segments underwent a disinfection process as described in the following sequence: the explants were immersed in 70% alcohol for 1 min; soon after, they were immersed in 1.5% sodium hypochlorite with 2 drops of neutral detergent for 10 min; at the end, the disinfected explants underwent triple washing with distilled, deionized and autoclave water (DDA) to completely remove the products from the surface of the tissues.…”

In vitro germination of Abricó-de-Macaco (Couroupita guianensis aubl.)zygotic embryos in different culture media and light spectra

“…In the trial with cajú do cerrado, the MS (50% and 25%) and WPM (100% and 50%) media were the most efficient in plant regeneration (ASSIS et al, 2012). Meanwhile, in caçari, 100% WPM was superior to the MS and JADS medium at concentrations of 25%, 50%, 75%, and 100% (ARAÚJO et al, 2016). Notably, for some woody plants, the higher concentration of nitrogen salts has a negative effect on in vitro development.…”