Micropropagation of Alocasia amazonica using semisolid and liquid cultures

Jo, U. A.; Murthy, Hosakatte Niranjana; Hahn, Eun-Joo; Paek, Kee-Yoeup

doi:10.1007/s11627-008-9103-8

Cited by 13 publications

(15 citation statements)

References 19 publications

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“…In liquid cultures, explants are continuously in contact with the medium, which enables a constant supply and high absorption of nutrients, as well as suitable aeration, which apparently enhance ex-plant growth. Improved growth of explants in liquid culture also has been reported for other Araceae species [e.g., A. amazonica (Jo et al, 2008a) and S. cannifolium (Dewir et al, 2006)]. Axillary shoot multiplication of philodendron is greatly influenced by cytokinin concentration (Table 1).…”

Section: Resultsmentioning

confidence: 57%

“…The proportions of auxins and cytokinins is a determinant for meristem formation (George, 1993). Previous studies have reported that the combination of cytokinins and auxins exerts a synergistic and positive influence on the shoot proliferation of Araceae plant species, for example, Aglaonema 'Valentine' (El-Mahrouk et al, 2016), Alocasia amazonica (Jo et al, 2008a), Philodendron 'Serratum' (Patel and Shah, 2004), and Spathiphyllum cannifolium (Dewir et al, 2006). Different MS medium salt strengths and sucrose concentrations were tested to identify the ideal conditions for stimulating shoot multiplication (Table 3).…”

Section: Resultsmentioning

confidence: 99%

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Micropropagation of Lacy Tree Philodendron (Philodendron bipinnatifidum Schott ex Endl.)

Alawaadh¹,

Dewir²,

Alwihibi³

et al. 2020

horts

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The present study aimed to optimize the micropropagation of lacy tree philodendron using shoot tip explants. Axillary shoot regeneration was investigated in Murashige and Skoog (MS) medium with different types and concentrations of plant growth regulators, varied levels of MS medium salt strength, sucrose concentration, and light intensity and culture type. Adding 6-benzylaminopurine (BAP; 1 mg·L−1) significantly increased shoot multiplication compared with other cytokinins, and the combination of cytokinins and auxins [indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA)], yielded more shoots than cytokinins alone, with the greatest number of axillary shoots (11.4 per explant) obtained using both BAP (1 mg·L−1) and IBA (0.5 mg·L−1). In addition, the use of half-strength salt concentrations significantly reduced shoot multiplication, and high sucrose concentrations (>30 g·L−1) reduced explant growth. High light intensity also reduced shoot multiplication and growth, owing to photoinhibition, and shoot multiplication was more efficient in gelled culture, whereas shoot growth was greater in liquid/bioreactor culture. The best rooting success (100%) and greatest root number and fresh weight were obtained using MS medium supplemented with NAA (1–2 mg·L−1). The resulting plantlets were successfully acclimatized, with a survival rate of 100%, and were morphologically similar to the mother plant.

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Section: Resultsmentioning

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Micropropagation of Lacy Tree Philodendron (Philodendron bipinnatifidum Schott ex Endl.)

Alawaadh¹,

Dewir²,

Alwihibi³

et al. 2020

horts

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“…Similarly, in Macotyloma uniflorum axillary shoots were promoted in BA alone or in (Varisai et al 1999). It was also reported in Alocasia amazonica that cytokinins are responsible for optimal shoot bud proliferation and the addition of auxins could not promote shoot proliferation efficiency (Jo et al 2008). This may be due to the interaction of auxins with cytokinins during morphogenetic events as reported by Sato and Mori (2001).…”

Section: Resultsmentioning

confidence: 97%

In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity

Jana¹,

Shekhawat²

2012

Biologia plant.

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An efficient in vitro regeneration protocol was developed for medicinally important aromatic plant Anethum graveolens. Nodal segments were cultured onto Murashige and Skoog (MS) basal medium supplemented with different auxins and cytokinins singly as well as in combinations. The optimum callus induction (93.33 %) was obtained on medium fortified with 2.2 µM N 6 -benzyladenine (BA) and 0.21 µM α-naphthaleneacetic acid. The best shoot regeneration (85.7 %) with 12.86 shoots per explant was achieved in two weeks when callus was subcultured on MS medium amended with 2.2 µM BA and 1.85 µM kinetin. The average length of regenerated shoots varied from 3.15 to 4.8 cm. The rooting of regenerated shoots was nearly 100 % on ¼ MS augmented with 4.9 µM indolebutyric acid with a maximum root length of 5.1 cm. Plantlets were successfully acclimatized with 60 % survival rate. During organogenesis, catalase and ascorbate peroxidase activity increased while superoxid dismutase activity decreased. Clonal fidelity of in vitro raised plants has been checked by random amplified polymorphic DNA using 10 selected decamer primers. It has been found that regenerated plants are true to type plants.

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“…TDZ, a synthetic cytokinin was used effectively in the induction of organogenesis (Casanova et al 2004) and embryogenesis (Visser et al 1992). Recently, TDZ is used universally even in micropropagation of ornamental plants (Escobar et al 2008;Jo et al 2008). To stimulate shoot proliferation more, BA, combined with IAA, was supplemented in the medium.…”

Section: Resultsmentioning

confidence: 99%

In vitro micropropagation of water hyacinth (Eichhornia crassipes)

Suh¹,

Park²,

Han³

2010

Journal of Plant Biotechnology

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This study was conducted to refine a micropropagation method of water hyacinth (Eichhornia crassipes) in vitro. When young shoots were cultured on media with various concentrations of BA or TDZ alone, LS medium containing 5.0 mgl-1 BA was found favorable for shoot proliferation from young shoots with a mean of 4.2 shoots. Using BA together with IAA, more shoots were obtained on LS medium containing 5.0 mgl-1 BA and 1.0 mgl-1 IAA with a mean of 5.7 shoots. In liquid medium, number of shoots and fresh weight per explant increased significantly. The best shoot proliferation and increasing of fresh weight were achieved on LS liquid medium containing 5.0 mgl-1 BA and 1.0 mgl-1 IAA with 6.9 shoots and more than 4,000 mg fresh weight. Of the different concentrations of LS salt, double strength of LS medium provided the highest shoot proliferation with 7.3 shoots, and fresh weight with 5,539 mg per explant. Shoot proliferation on LS medium containing 50 gl-1 sucrose had better results with 8.7 shoots and 5,979 mg per explant in fresh weight than other conditions. In conclusion, the optimal level for shoot proliferation and biomass increase of water hyacinth was attained with the application of the double strength of LS medium containing 5.0 mgl-1 BA, 1.0 mgl-1 IAA and 50 gl-1 sucrose.

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Micropropagation of Alocasia amazonica using semisolid and liquid cultures

Cited by 13 publications

References 19 publications

Micropropagation of Lacy Tree Philodendron (Philodendron bipinnatifidum Schott ex Endl.)

Micropropagation of Lacy Tree Philodendron (Philodendron bipinnatifidum Schott ex Endl.)

In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity

In vitro micropropagation of water hyacinth (Eichhornia crassipes)

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