Micropropagation of a difficult-to-root weeping mulberry (Morus alba var. Shidareguwa): A popular variety for ornamental purposes

Aroonpong, Piyathida; Chang, Jung-Chin

doi:10.1016/j.scienta.2015.08.019

Cited by 17 publications

(12 citation statements)

References 24 publications

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“…The average total root dry mass was twice as high in the treatment 3 compared to treatment 2 and control. This is consistent with other studies demonstrating the effects of auxin in increasing root mass and number (Aslam et al, 2013;Aroonpong and Chang, 2015).…”

Section: Phase 2: Rooting Phasesupporting

confidence: 93%

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Antoniazzi¹,

Ferrari²,

Nascimento³

et al. 2016

Afr. J. Biotechnol.

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Curcuma longa L., from the Zingiberaceae family, generally reproduces through its rhizomes, which are also utilized for therapeutic purposes because they are rich in terpenoids. Its conventional propagation has low efficiency due to the small number of seedlings and their contamination by pathogens. Therefore, this study aimed to evaluate the influence of growth regulators on the development of in vitro-cultivated C. longa as well as to determine their influence on DNA content and foliar anatomy. Shoots were inoculated in MS culture medium with the addition of 30 g/L of sucrose and 6.5 g/L of agar, and a pH adjusted to 5.8. Two assays were built to study the multiplication and rooting phases of growth. The first assay evaluated the influence of eight concentrations of cytokinins and auxins on the multiplication phase. Leaf samples were analyzed for DNA content through flow cytometry, utilizing two reference standards, green pea, and tomato. Characteristics of leaf anatomy were also measured in four time periods. The second assay analyzed the influence of six auxin concentrations on the rooting phase. The first assay showed that the root systems grew more in treatment 3 (4.44 µM benzylaminopurine [BAP], 0.46 µM kinetin [KIN]) and reached greater dry mass in T8 (8.88 µM BAP, 0.92 µM KIN, 2.16 µM naphthalene acetic acid [NAA]). The largest fresh matter of the main shoot was found in T2 (4.44 µM BAP). The estimated DNA content varied depending on the presence of supplemental growth regulators, from 2.38 to 2.77 pg, and was greater in T4 (4.44 µM BAP, 1.08 µM NAA) and T5 (4.44 µM BAP, 0.46 µM KIN, 1.08 µM NAA). Results from the latter two treatments were not significantly different. Estimates of DNA content were precise, as indicated by coefficients of variation that were much lower than 5%. The results also showed quantitative variation of evaluated anatomical traits. In general, there was a thin epidermis layer with rectangular cells, followed by parenchyma with octahedral cells and differentiated xylem and phloem. In leaf parenchyma, the presence of idioblasts containing phenolic compounds was observed in all growth stages. In the rooting phase, the supplementary auxins affected the dry matter of the aerial part and roots. The highest averages were observed in treatments with 2.0 µM NAA.

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Section: Phase 2: Rooting Phasesupporting

confidence: 93%

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Antoniazzi¹,

Ferrari²,

Nascimento³

et al. 2016

Afr. J. Biotechnol.

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“…[20]; Morus alba var. shidareguwa showed the best shoot multiplication treatment using 1.0 mg•L -1 BAP at 30 days [8]. The new shoots were cultured on PGR-free after 30 days, and did not show alterations in their leaf and stem morphology rather they had similar feature to O. grandiflora plants in their natural environment.…”

Section: In Vitro Shoot Multiplicationmentioning

confidence: 91%

“…Aroonpong and Chang [8] also found that auxins were unnecessary for in vitro rooting of 'Shidareguwa' mulberry. Similar result was found in the PGR-free rooting medium of O. grandiflora (Figs.…”

Section: In Vitro Rootingmentioning

confidence: 99%

“…On the other hand, the use of biotechnology through plant micropropagation techniques allows the rapid production of plants at low cost, and in limited spaces [8]. These techniques are based on somatic cell culture, or tissue and organs culture under controlled conditions, to produce a high number of plantlets free of microorganisms, and in short times compared with the conventional propagation [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…These techniques are based on somatic cell culture, or tissue and organs culture under controlled conditions, to produce a high number of plantlets free of microorganisms, and in short times compared with the conventional propagation [9,10]. Thus, micropropagation is becoming an alternative for multiplication and preservation of endangered species, especially wild medicinal plants; and it can get high quality seedlings free for viral, fungal and bacterial pathogen [8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro Propagation of Oreocallis grandiflora (Lam.) R. Br., A Medicinal Threatened Plant

Olivera-Gonzales¹,

Yldefonzo²,

Mestanza³

et al. 2017

ARRB

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Transgenic Mulberry (Morus Spp.) for Stress Tolerance: Current Status and Challenges

Sarkar

Raghunath

Sivaprasad³

et al. 2023

Compendium of Plant Genomes

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Micropropagation of a difficult-to-root weeping mulberry (Morus alba var. Shidareguwa): A popular variety for ornamental purposes

Cited by 17 publications

References 24 publications

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

In vitro Propagation of Oreocallis grandiflora (Lam.) R. Br., A Medicinal Threatened Plant

Transgenic Mulberry (Morus Spp.) for Stress Tolerance: Current Status and Challenges

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