Micropropagation of a Casuarina hybrid (Casuarina equisetifolia L. × Casuarina glauca Sieber ex Spreng) following facilitated seed germination

Abstract

A suitable protocol for micropropagation of Casuarina hybrid, Casuarina equisetifolia L. 9 Casuarina glauca Sieber ex Spreng (C. e. 9 C. g.), was developed. When seeds without seed coats were cultured on 4 germination media, the optimal seed germination percentage (91%) was obtained on 0.8% agar solidified water medium. Shoot multiplication was achieved by culturing 2-cm long epicotyls, excised from germinated seedlings, on MS (Murashige and Skoog 1962) basal medium supplemented with BA (6-benzylaminopurine) at 4.4, 8.8, 17.8 and 35.6 lM. The greatest percentage of axillary bud sproutings (87.5%), mean number of sprouts per explant (3.8), and shoot length (3.2 cm) were achieved on MS medium supplemented with 17.8 lM BA. MS medium supplemented with 4 different concentrations of IBA (indole-3-butyric acid) (4.3, 8.7, 13.0 and 17.4 lM) were used for rooting of in vitro grown shoots. The highest rooting percentage (65.6%), mean number of roots per explant (2.5) and mean length of roots per explant (1.6 cm) was achieved at 13.0 lM IBA. Rooted shoots grew well after transfer to a substrate of peat and pinebark (7:3) in the greenhouse.

In Vitro Shoot Proliferation and Root Induction of Shoot Tip Explants from Mature Male Plants of Casuarina cunninghamiana Miq.

“…PGR-free medium served as the control. We selected BA because it is a commonly used cytokinin that was effective in inducing shoot proliferation from epicotyl explants excised from in vitro-germinated seedlings of a Casuarina hybrid (Shen et al, 2009b); TDZ is also a cytokinin known to induce axillary bud proliferation in recalcitrant species (Lu, 1993); and GA 3 can aid in breaking bud dormancy (Suzuki and Kitano, 2006).…”

“…We have established a protocol for micropropagation of a Casuarina hybrid (C. equisetifolia L. • C. glauca Sieber ex Spreng) using epicotyl explants excised from seeds germinated in vitro. Shoot proliferation was obtained on Murashige and Skoog (MS) medium (Murashige and Skoog, 1962) supplemented with 6-benzylaminopurine (BA) at 0 to 35.6 mM and root induction on MS medium supplemented with indole-3butyric acid (IBA) from 4.3 to 17.4 mM (Shen et al, 2009b). However, this in vitro protocol was not successful using shoot tips taken from mature male C. cunninghamiana trees in our preliminary experiment.…”

Διερεύνηση μεθόδων in vitro πολλαπλασιασμού, της οικοφυσιολογίας του σπόρου και των φυλογενετικών σχέσεων των ειδών Arbutus της Ελλάδας

“…(1991), από βλαστούς νεαρών σποροφύτων που είχαν βλαστήσει in vitro, όπως και τα Εucalyptus grandis x E. urophylla (Cid et al, 1999) και Ilex aquifolium L. (Majada et al, 2000). Οι Shen et al, (2009)…”