Establishment of an in vitro plant regeneration protocol for Casuarina cunninghamiana Miq. via indirect organogenesis

Jiang, Qingbin; Zhang, Yong; Chen, Zhong; Zeng, Bo; Bogusz, Didier; Franche, Claudine

doi:10.1007/s11056-011-9277-5

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…The effective selection of explants is the basic requirement for any plant propagation protocol (Jiang et al 2012). Cultures of C. guianensis were established from the embryos of the seeds, nodal segments and apical shoots.…”

Section: Resultsmentioning

confidence: 99%

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

Shekhawat¹,

Manokari²

2016

Physiol Mol Biol Plants

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Section: Resultsmentioning

confidence: 99%

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

Shekhawat¹,

Manokari²

2016

Physiol Mol Biol Plants

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“…Hence, cytokinins particularly 1.5 mg/l BAP with 0.5 mg/l NAA combination acts as trigger and was more effective for shoot regeneration from callus. Similarly the synergetic effect of BAP with NAA were observed in Glinus lotoides (L.) 32 , Eclipta alba L 3 , Casuarina cunninghamiana Miq 14 and in Saussurea obvallata1 8 . However In Psoralea corylifolia higher number of shoots (6.15 shoots) were obtained on 14 μM/l BAP with 10.0 μM/l NAA and 10.0 ìM/l KN from leaf derived callus 25,26 .…”

Section: Callus Regenerationmentioning

confidence: 58%

Callus Induction and Organogenesis from Leaf Explants of Psoralea corylifolia Linn: An Endangered Medicinal Plant

Gulam Nabi,

Shrivastava,

Ayoub

2016

Food & Pharma Intl.

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A callus induction and plant regeneration protocol was developed from leaf explants of the Psoralea corylifolia Linn. Explants were cultured on MS medium supplemented with different plant growth regulators (PGRs) i.e. 2, 4-D, BAP and KN. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearance of callus and the potential of callus differentiation. 2, 4-D (1.0 mg/l) alone and 1.0 mg/l of 2, 4-D with 0.5 mg/l KN have been found highly effective in callusing with quite good in texture and friable in nature i.e. 65.5 % to 75.0 % respectively. After the callus formation the induced callus were sectioned into small pieces and transferred on MS media supplemented with BAP and KN separately or BAP and KN with NAA, IBA and IAA. MS medium containing 1.5 mg/l BAP with 0.5 mg/l NAA was found to be the best medium for maximum in vitro response i.e. (80.0 %) shooting and multiple shoots (6.0) with maximum 7.5 cm in length. Best rooting was achieved on full strength MS medium fortified with 1.0 mg/l IBA respectively.

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“…For the establishment of in vitro cultures, the type of culture medium, source and age of explants, surface disinfection procedures, microbial contamination, and environmental factors are all critical. A review of the literature suggests that any plant regeneration methodology requires careful selection of explants and growth regulators [40]. Preliminary studies for the formation of in vitro shoot cultures using shoot tips were carried out in the current study.…”

Section: Multiplication Stage Influence Of Various Concentrations Of ...mentioning

confidence: 99%

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

Nower

2022

Journal of Genetic Engineering and Biotechnology

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Background Dimocarpus longan is a tropical tree that produces edible fruit. It is a neglected plant species that is listed as near threatened. In spite of its economic value, the propagation of longan cultivar using conventional methods is extremely difficult. The goal of this research is to produce and conserve this plant through in vitro propagation. Results In order to form new shoots, sterilized shoot tip explants were cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (BA) or 2-isobentenyl-adenine (2ip). For direct organogenesis, young leaves of new shoots were cultured on MS medium fortified with various concentrations of Thidiazuron (TDZ) or 6-(4-Hydroxy-3-methylbut-2-enylamino purine) (Zeatin). Gibbrellic acid (GA3) at different levels alone or in combination was used for shoot elongation. Also, indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) were used for root formation. MS medium supplemented with 1.00 mg/l 2ip was suitable for inducing axillary shoots from shoot tips (4.0 axillary shoots/explant). The highest significant 76% and numbers of adventitious buds from leaf base were achieved on MS medium containing 1.0 mg/l TDZ. These buds developed into the longest plantlets on GA3 at 3.0 mg/l and rooted well in ½MS containing 1.50 mg/l IBA plus 0.50 mg/l (NAA). About 70% in vitro plants were successfully acclimatized. The AFLP profile illustrated the genetic stability of gene expression action. The amplified fragment length polymorphisms (AFLPs) profile illustrated the progenies were extremely similar to the mother plants. According to our findings, MS medium containing 25 ppm salicylic acid (SA) and 5 ppm methyl jasmonate (MeJA) produced the highest percentage of apigenin in longan calli (77.09 and 2.637%, w/w). Conclusion A successful and efficient micropropagation protocol has been developed and described here for the first time, and it will be very useful for the clonal propagation and conservation of the near-threatened Dimocarpus longan plant. Micropropagated plants are genetically identical to the donor plant using the AFLP technique. The usefulness of salicylic acid and methyl jasmonate as elicitors for increasing in vitro production of secondary metabolites in plants is demonstrated in this work.

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Establishment of an in vitro plant regeneration protocol for Casuarina cunninghamiana Miq. via indirect organogenesis

Cited by 11 publications

References 19 publications

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

Callus Induction and Organogenesis from Leaf Explants of Psoralea corylifolia Linn: An Endangered Medicinal Plant

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

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