Micropropagation and seed cryopreservation of the critically endangered species Tennessee yellow-eye grass, Xyris tennesseensis Kral

Johnson, Timothy R.; Cruse-Sanders, J. M.; Pullman, Gerald S.

doi:10.1007/s11627-011-9420-1

Cited by 12 publications

(5 citation statements)

References 15 publications

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“…This strength was selected to avoid the high ionic strength present in full-MS salts and to better simulate the low-mineral conditions that may occur in wet soils. Northcutt et al (2012) and Johnson et al (2012) used the same salt mix to germinate seeds and micropropagate shoots from several Sarracenia species and Xyris tennesseensis, species that also live in wet, nutrient-poor environments.…”

Section: Discussionmentioning

confidence: 99%

Seed cryopreservation and micropropagation of the critically endangered species swamp pink (Helonias bullata L.)

Perullo

Determann²,

Cruse-Sanders³

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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Helonias bullata, commonly known as swamp pink, is a perennial rhizomatous herb native to the eastern coast of the United States that is found mainly in forested wetlands, swamps, and mountain bogs. Habitat destruction has caused H. bullata to become rare, and it is federally listed as threatened. Protocols were developed for in vitro germination, shoot micropropagation, shoot establishment in soil, and seed cryopreservation that will assist in the safeguarding and conservation of dwindling natural populations. Seeds were germinated in vitro on plant growth regulator (PGR)-free 1/3-strength Murashige and Skoog (MS) medium after seed sterilization in H 2 O 2 and used for establishment of shoot cultures. Shoot multiplication and elongation occurred on 1/3-strength MS medium containing 0.25-1 mg/l kinetin or 1-2 mg/l trans-zeatin followed by growth on PGR-free medium. All shoots (100%) rooted when planted directly into potting media. Rapid-immersion seed cryopreservation resulted in statistically significant increases in germination in vitro or in potting mix compared to control treatments. H. bullata seeds share characteristics of both orthodox and recalcitrant seeds including rapid loss of viability over time, survival at 5°C, and survival after cryopreservation. Therefore, they are most accurately classified as intermediate-type seed.

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Section: Discussionmentioning

confidence: 99%

Seed cryopreservation and micropropagation of the critically endangered species swamp pink (Helonias bullata L.)

Perullo

Determann²,

Cruse-Sanders³

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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“…In vitro techniques are reliable alternatives not only for the conservation of endangered and/or endemic species for further reintroduction programs (Holobiuc et al, 2009;Marriott and Sarasan, 2010;Johnson et al, 2012) but also for the revegetation and stabilization of polluted wastes (Muszyńska and Hanus-Fajerska, 2017;Muszyńska et al, 2018). Both, the development of appropriate conservation programs for plant species and the preservation of their natural variability require thorough genetic variability studies in natural populations (Halmagyi and Butiuc-Keul, 2007).…”

Section: Introductionmentioning

confidence: 99%

Acta Biologica Cracoviensia s. Botanica

Cristea,

Besenyei,

Jarda

et al. 2019

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Rare and endemic plant species represent important components of plant biodiversity which require protection to ensure their sustainable conservation. Cerastium banaticum (Rochel) Heuff. is such an endemic and rare species from Romania, for which the genetic variability of two natural populations was studied by SSR markers. Shannon's information index revealed low levels of genetic diversity in both populations (I = 0.296). As the first attempt in a conservation program a reproducible micropropagation protocol was established starting from seeds, followed by multiplication, rooting, and ex vitro acclimatization. Among the various plant growth regulators tested the highest multiplication coefficient was achieved on a culture medium with 0.5 mg L -1 6-furfurylaminopurine (K) and 1 mg L -1 α-naphthaleneacetic acid (NAA). On this PGRs concentration a number of 26.6 shoots/individual explant with a mean length of 7.9 cm for new generated shoots was registered. The highest number of roots/individual initiated shoot was 2.6 and it was recorded on a culture medium with 0.5 mg L -1 2-isopentyl-adenine (2iP) and 0.1 mg L -1 NAA. The outdoor acclimatization was successfully performed in a specially designed rocky area in the 'Alexandru Borza' Botanical Garden, Cluj-Napoca (Romania).

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“…The efficient approach to plant conservation implies in situ and ex situ conservation, having as a main target the maintenance of appropriate genetic diversity of the concerned species. Ex situ conservation is a viable alternative for taxa that are highly endangered in their natural environment or may complement in situ conservation methods (Ramsay et al, 2000;Marriott and Sarasan, 2010;Johnson et al, 2012). In vitro multiplication and reintroduction into natural habitats of rare, endangered, or endemic plant species allows the preservation of these species and expands the natural population.…”

Section: Introductionmentioning

confidence: 99%

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Jarda¹,

Butiuc-Keul²,

Höhn³

et al. 2014

Turk J Biol

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Ex situ conservation of Dianthus giganteus subsp. banaticus was obtained by in vitro culture on different culture media supplemented with phytohormones. Assessment of somaclonal variability over a period of preservation by in vitro culture is required prior to introduction and acclimatization in natural habitats. In this study we used 2 types of molecular markers, ISSR and multilocus SSR markers, which allow comparison of different alleles, to assess the somaclonal variability of endemic taxa Dianthus giganteus subsp. banaticus after a period of 24 months of preservation by in vitro culture. The assessment of somaclonal variability of D. giganteus subsp. banaticus shows some changes in the absence/presence of certain markers in some individuals.

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Micropropagation and seed cryopreservation of the critically endangered species Tennessee yellow-eye grass, Xyris tennesseensis Kral

Cited by 12 publications

References 15 publications

Seed cryopreservation and micropropagation of the critically endangered species swamp pink (Helonias bullata L.)

Seed cryopreservation and micropropagation of the critically endangered species swamp pink (Helonias bullata L.)

Acta Biologica Cracoviensia s. Botanica

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

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