Micropropagation and protein profile analysis by SDS-PAGE of Gracilaria changii (Rhodophyta, Solieriaceae)

Investigating the Effects of Exogenous Factors on Growth, Photosynthetic Pigments and Bud Induction in Gracilaria corticata var. cylindrica under In vitro Conditions

“…Ding et al, (2013) also observed negative growth at high salinity in Gracilaria species. In present experiment, the optimum salinity for growth of explants was observed to be 30 ppt in both the media (S 2 & A 2 ), which is in line with the earlier reports (Bird and McLachlan, 1986;Ding et al, 2013;Jong et al, 2015). Further, A 2 media at this salinity provided better environment for culture growth, which is in agreement with Kaladharan et al, (2003), who reported suitability of artificial seawater for in vitro culture of Gracilaria species.…”

“…Both suboptimal and supraoptimal levels of media were not conducive for culture growth as bleaching of explants was observed in these concentrations of FSAAS media. Although Gracilaria has been reported to grow at low saline conditions (Bird and McLachlan, 1986), reduced growth (Graham and Wilcox, 2000;Jong et al, 2015) and bleaching (Jong et al, 2015) have been noticed. Loss of thallus rigidity has been reported at low salinity levels (Kumar et al, 2010).…”

Genetic Identification and Mass Propagation of Economically Important Seaweeds

“…The optimized culture medium for economically important K. alvarezii was discovered to be seawater enriched with 50% of PES solution, whereas enrichments with 50% of VS and 50% of F/2 solutions were found not effective for K. alvarezii cultures [15]. Besides, G. changii cultured in 25% of PES enriched seawater was revealed propagating well and demonstrated promising growth rate [80]. Although some rhodophytes have been reported to grow well in VS and F/2 media [89,90], the difference in media used may be due to the source of different explants or different genotype of explants.…”

“…However, challenges including lack of optimized protocols to obtain axenic cultures and regeneration of explants have limited the widespread use of tissue culture technology in commercial seaweed production. The efficacy of seaweed tissue culture is depends on the effective manipulation of endogenous (age, source, developmental stage, and physiological state of explants) and exogenous (media composition, light, salinity, pH, and temperature) factors [80,81]. Current researches have been strategized to improve the culture conditions for mass production of high-quality laboratory seedlings to enhance the overall productivity of seaweed cultivation [3,9,14,15].…”

“…Salinity is reported to be one of the factors affecting the growth [15,80] and exerting strong influences on the photosynthetic capacity [117,118] of the cultured seaweeds. Prolonged exposure to low salinity may induce stress that led to reduced photosynthetic efficiency, inhibited cell division, and subsequently result in stunted growth and declined in growth rate [80].…”