Investigating the Effects of Exogenous Factors on Growth, Photosynthetic Pigments and Bud Induction in Gracilaria corticata var. cylindrica under  In vitro Conditions

Anuraj, A.; Waman, Ajit Arun; Prakāsh, Chandra; Roy, S. Dam; Viji, M. M.; Baidya, Manoj; Chadha, Narinder Kumar

doi:10.20546/ijcmas.2017.609.398

Cited by 2 publications

(1 citation statement)

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“…Also, Mutui et al, (2012) sprayed various growth regulators on Pelargonium×hortorum and found that metatopolin had significant effects in increasing the total chlorophylls and it is very active in reducing the leaf senescence. Moreover, Anuraj et al, (2017) stated that supplemented the in vitro growth medium with 2.5 mg/l meta-topolin improved the tolerance of Gracilaria corticata var. cylindrica to the salinity condition and increased the total chlorophylls and carotenoids.…”

Section: Total Chlorophylls In Micro-shootsmentioning

confidence: 99%

Indirect Micropropagation of Gerbera jamesonii Bolus cv. Winter Queen through Callus Redifferentiation

Kasem

2018

Journal of Plant Production

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during 2015/2016 on Gerbera jamesonii Bolus cv. Winter Queen for studying the indirect micropropagation of this important pot and cut flower plant in Egypt. For callus induction, unopened flower buds(10 days old) were cultured after preparation on MS medium fortified with either 2,4-D or NAA (0.5, 1.0, 2.0, 4.0 and 6.0 mg/l) each alone. The calli were transferred on media supplemented with either BAP or meta-topolin (0.0, 0.3, 0.6, 1.2 and 2.4 mg/l) each alone with 0.5 mg/l NAA as a fixed concentration for redifferentiation. Moreover, shoots were transferred to media supplemented with NAA (0.0, 0.5, 1.0, 1.5 and 2.0 mg/l) with 0.3 mg/l meta-topolin for the in vitro rooting. Results showed that 6.0 mg/l 2,4-D produced the heaviest callus fresh weight (7.22 g), and decreased the callus induction time (17.50 days), but the callus texture was compact and creamish to brown in color. In addition, the lower concentrations from either 2,4-D (1.0 and 2.0 mg/l) or NAA (0.5 to 1.0 mg/l) produced a nodular greenish calli with greenish color. Also, supplying the redifferentiation media with 1.2 and 2.4 mg/l meta-topolin or 2.4 mg/l BAP, significantly reduced the redifferentiation time, increased the multiplication percentage and micro-shoots number. Moreover, ½ strength MS medium fortified with 2.0 mg/l NAA+0.3 mg/l meta-topolin gave the maximum roots number, rooting% and reduced the roots initiation time. Finally, well rooted plantlets were transferred to a mixture of peatmoss+perlite+clay (2:1:1 v/v) produced a survival percentage of 92.90% with a plantlets height of 4.61cm and 4.50 leaves / plantlet on average.

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Section: Total Chlorophylls In Micro-shootsmentioning

confidence: 99%