Micropropagation and Effect of Phloroglucinol on Rooting of Diospyros crassiflora Hiern

Tchouga, Alvine Ornella; Deblauwe, Vincent; Djabou, Stephanie Astride Mouafi; Forgione, Giovanni; Hanna, Rachid; Niemenak, Nicolas

doi:10.21273/hortsci14556-19

Cited by 6 publications

(3 citation statements)

References 19 publications

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“…The genus Diospyros is a group of plants that has received little attention compared to other genera. However, some studies have focused on two particular species, for example Diospyros kaki Thunb (Tao and Sugiura 1992)and D. crassi ora Hiern (Tchouga et al 2020). In these studies, regeneration of nodal segments with zeatin was used as an effective method to produce axillary shoots.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Culture and Phytochemical Analysis of Diospyros Anisandra

Santana

Pool

Alamillo

et al. 2023

Preprint

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Diospyros anisandra is a small tree that contains metabolites with antitubercular, antibacterial, larvicidal, antioxidant, antihistaminic, and antiviral properties in its leaves, stem bark, and roots. However, this plant is slowly growing and takes about 10 years to reach maturity in situ. Therefore, it makes it an ideal model to apply tissue cultures and thus be able to have the metabolites of interest as required. In the present work we report for the first time, the establishment and comparison of three different protocols of in vitro culture of D. anisandra. This work aimed to establish stable in vitro cultivation conditions with seeds, leaves and nodal segments. They were cultured in Murashige and Skoog (MS) medium supplemented with plant growth regulators such as Gibberellic acid (GA3), zeatin, 6-benzylaminopurine (BAP), and 2,4-dichlorophenoxyacetic acid (2,4-D). The results showed that a germination efficiency of 73% was achieved in seeds with 57.8 µM GA3. In leaf regeneration, 80% regeneration of embryonic mass was achieved with 28.9 µM of BAP and zeatin. Finally, in nodal segments, an 85% efficiency in axillary shoot regeneration was achieved with 28.9 µM zeatin. On the other hand, the hexane extract of the plant segments of all treatments was analyzed by thin layer chromatography and gas chromatography-mass spectrometry (GC/MS). The main compounds detected are plumbagin, stigmasterol, β-sitosterol, taraxasterol, vitamin E, betulinic acid among others in the three systems studied, therefore, we can conclude that success was obtained in generating a cultivation method that allows us to have these compounds in less than 1 year of growth.

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Section: Discussionmentioning

confidence: 99%

In Vitro Culture and Phytochemical Analysis of Diospyros Anisandra

Santana

Pool

Alamillo

et al. 2023

Preprint

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“…IBA cũng là chất ĐHST rất thích hợp trong sử dụng tạo rễ đối với nhiều cây dược liệu (Rahmat, Kang, 2019). Tuy nhiên, ở một số đối tượng thực vật, IBA (kể cả IAA) hoàn toàn không thích hợp, ví dụ mảnh lá Andrographis paniculata trên môi trường MS chỉ tạo được rễ bất định khi sử dụng NAA (1 mg/L) (Sharmaa et al, 2013); cũng nhận thấy chồi Diospyros crassiflora không tạo rễ bất định trên môi trường ½MS có IBA (2,5 -19,6 µM) (Tchouga et al, 2020). Nói chung, loại và nồng độ auxin thích hợp cho cảm ứng tạo rễ và kéo dài rễ bất định phụ thuộc chủ yếu vào loài thực vật (Nandagopal, Kumari, 2007).…”

Section: ảNh Hưởng Của Naa/iba đếN Cảm ứNg Tạo Rễ Bất địNh Trực Tiếp Từ Lmtb Láunclassified

Direct induction of adventitious root in Schefflera octophylla (Lour.) Harms from leaf explants cultured in vitro

Luy¹,

Ho²,

Lệ³

2021

Vietnam J. Biotechnol.

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Schefflera octophylla (Lour.) Harms is a precious plant species belonging to the Araliaceae family. All parts of plant have been used to create products for human health. In tissue culture of medicinal plants, the induction and multiplication of adventitious root of Schefflera octophylla for biomass collection have been studied. In this report, results on induction of adventitious root from leaf explants cultured in vitro of this plant species were presented. Leaf disks (~ 10 x 10 mm), leaf transverse - thin cell layers (t-TCLs) (~ 3 x 10 mm) were cultured on different mineral media MS, ½MS, B5, SH with NAA (0 - 5 mg/L), sucrose (0 - 50 g/L) and light intensity (0 - 4,000 lux). The results showed that, 30 days after culturing on ½MS solid medium plus 3 mg/L NAA, and 30 g/L sucrose in 4,000 lux light condition, direct formation of adventitious root was best from leaf disks, t-TCLs with rooting rate (%) 100, 100; root number/sample 68.80, 21.96; root lenght (mm) 16.53, 15.53, respectively. Leaf disk culture resulted in better rooting than t-TCL culture in two criteria of root number and root length. Morphological and histological observations of adventitious root primordia formation in the leaf disk were also performed. This is the first report on direct formation of adventitious root by in vitro culture of leaf disks/t-TCLs in Schefflera octophylla with very high efficiency, creating basis for further studies on root biomass multiplication for production of bioactive compounds.

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“…To meet the needs of the population with high-quality fruits in fresh and processed form according to a scientifically based nutritional norm (85 kg of fruit per person per year), it is necessary to significantly increase the production of these products [3]. The solution of this problem is carried out due to the intensification of the fruit growing industry [4]. At the present stage, intensification is based on the introduction into production of new highly productive varieties of fruit species with limited growth, new designs of tree crowns, as well as on the use of low-growing clonal rootstocks [5].…”

Section: Introductionmentioning

confidence: 99%

Growing a low-growth clone planting material of cherry from green cuttings

2023

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We dealt with the study of the ability of different varieties of cherries, cherries and vegetatively propagated rootstocks to reproduce by green cutting explants in artificial substrates in buildings with a controlled microclimate inside. As varieties in the experiment, such varieties of cherries as Shpanka black, Podbelskaya, Shubinka and Lyubskaya, cherries Revershon, Bakhor, and Drogana yellow were used. Of the vegetatively propagated rootstocks, the following were studied: P-1, P-2, P-3, PN, VTS-13, VP-1, ORP-2, ORP-3, VSL-2 (Krymsky 5), SAV-6R. Of the used cherry varieties, the best rooting of green cuttings was possessed by such cherry varieties as Shpanka black, Podbelskaya and Shubinka, with a quality index of cuttings rooting in the substrate of 70.1 to 83.5%. In sweet cherry varieties, this indicator was 2.5 times lower and generally did not exceed 30.2%. Vegetatively propagated rootstocks provided high quality results of rooting of cuttings explants. In almost all of these rootstocks, the rooting rate of green cuttings did not decrease below 72%. The average rooting rate of rootstock cuttings was 85.7%. This indicates that these rootstocks can be used for industrial cultivation of stone fruit seedlings.

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Micropropagation and Effect of Phloroglucinol on Rooting of Diospyros crassiflora Hiern

Cited by 6 publications

References 19 publications

In Vitro Culture and Phytochemical Analysis of Diospyros Anisandra

In Vitro Culture and Phytochemical Analysis of Diospyros Anisandra

Direct induction of adventitious root in Schefflera octophylla (Lour.) Harms from leaf explants cultured in vitro

Growing a low-growth clone planting material of cherry from green cuttings

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