Microplate screening assay for the detection of arsenite-oxidizing and arsenate-reducing bacteria

Simeonova, Diliana D.; Lièvremont, Didier; Lagarde, Florence; Müller, Daniel; Groudeva, V.; Lett, Marie‐Claire

doi:10.1016/j.femsle.2004.06.040

Cited by 43 publications

(46 citation statements)

References 9 publications

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“…Inorganic arsenic detection was carried out through a modification of the silver nitrate (AgNO 3 ) technique described by Simeonova et al (2004). Experiments were carried out using basal medium supplemented with arsenate (0.1 mM) or arsenite (0.1 mM) as positive controls and basal medium with roxarsone (0.5 mM) and without arsenical inorganic compounds was used as negative control.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of an aerobic bacterial consortium able to degrade roxarsone

Guzmán‐Fierro

Moraga

León

et al. 2014

Int. J. Environ. Sci. Technol.

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Roxarsone is an organoarsenical compound used as food additive in the poultry industry. Roxarsone has the potential risk to contaminate the environment, mainly by the use of poultry industry manure as fertilizer, releasing inorganic arsenic to the soil and water. The aim of this work was to isolate and characterize a bacterial consortium capable to degrade roxarsone under aerobic conditions. A bacterial consortium was cultured from a soil sample obtained from a field fertilized with poultry litter containing roxarsone. The consortium was cultured in the presence or absence of roxarsone. Roxarsone degradation and growth kinetics were determined by incubation of the bacterial consortium in the presence of roxarsone at room temperature and under aerobiosis. Both consortiums were characterized molecularly by denaturing gradient gel electrophoresis analysis and metabolically using Biolog Ecoplates. Inorganic arsenic was assessed by precipitation with silver nitrate. The consortium was also analyzed by scanning electron microscopy. The results showed that growth rate of the bacterial consortium was 1.4-fold higher in presence of roxarsone and 81.04 % of the roxarsone was transformed after 7 days of incubation. Molecular characterization revealed the presence of different bacterial groups, being alphaproteobacteria and firmicutes the groups that showed the highest count in both consortiums. The metabolic profile of the consortium did not change in the presence of roxarsone, but it showed a greater ability to oxidize amines, suggesting production of functional amines to decrease the stability of the aromatic ring resonance energy, the principal problem associated with aromatic compounds degradation.

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Section: Methodsmentioning

confidence: 99%

Isolation and characterization of an aerobic bacterial consortium able to degrade roxarsone

Guzmán‐Fierro

Moraga

León

et al. 2014

Int. J. Environ. Sci. Technol.

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“…These strains may be the non-transforming strains or they may be utilizing arsenate by any another metabolic process. Earlier also a number of non-transforming bacterial strains present in environmental sample belonging to β or γ-proteobactor groups have been reported 9 . Bacterial cultures without arsenate (Fig.…”

Section: Resultsmentioning

confidence: 99%

A rapid colony screening method for the detection of arsenate-reducing bacteria

et al. 2007

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A rapid and simple method has been developed for the detection of arsenate reducing bacteria based on the presence of arsenite [As (III)], the end product of anaerobic arsenate [As (V)] respiration. Confirmation of As (III) fi product is made by the reduction of starch-iodine complex. The method can be used over a large pH range (5.5-9.0) and can easily be determined at arsenite concentration as low as 0.025 mM. Major advantages of this technique are that a large number of samples can be analyzed easily at a time.

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“…Arsenic-tolerant bacteria and their ability to oxidize As (III) to As (V) or reduce As (V) to As (III) were verified using the AgNO 3 method described by Simeonova et al (2004). Isolated strains grew in 50 mL of MS medium without arsenite in the dark for 72 h at 30 ± 2 °C and 200 rpm.…”

Section: Isolation and Selection Of Arsenite-oxidizing Bacteriamentioning

confidence: 99%

“…Las bacterias tolerantes al arsénico y su habilidad para oxidar As (III) a As (V) o reducir As (V) a As (III) se verificaron mediante el método AgNO 3 descrito por Simeonova et al (2004). Las cepas aisladas crecieron en 50 mL de medio MS sin arsenito en la oscuridad durante 72 h a 30 ± 2 °C y a 200 rpm.…”

Section: Determination Of the Enzymatic Activity Of The Arsenite Oxidunclassified

Bioprospección de bacterias oxidantes de arsenito de suelo de la Comarca Lagunera

Rangel-Montoya¹,

Balagurusamy²

2015

Rev Cha Se Cie For y del Amb

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A rsenic is one of the most toxic metalloids present in the environment and prolonged exposure to this metal causes chronic health effects. Therefore, the search for environmentally-friendly alternatives for the treatment of arsenic-contaminated water and soil is important. In this study, bacterial strains were isolated from arseniccontaining soils in the Lagunera region to analyze those with arsenite-oxidizing abiliity. Strains 04-SP1qa and 14-SP1qh with chemolithoautotrophic and chemoheterotrophic metabolism, respectively, had greater activity of the arsenite oxidase enzyme. The optimum growth conditions and enzymatic activity of these strains were investigated. Strain 04-SP1qa had specific enzymatic activity of 0.162 µmol·min Resumen E l arsénico es uno de los metaloides más tóxicos presente en el ambiente y la exposición prolongada a este metal causa efectos crónicos en la salud. Por ello, la búsqueda de alternativas amigables con el medio ambiente, para el tratamiento de agua y suelos contaminados con arsénico es importante. En este trabajo se aislaron cepas bacterianas de suelos con presencia de arsénico en la Comarca Lagunera, para analizar aquellas con capacidad oxidante de arsenito. Las cepas 04-SP1qa y 14-SP1qh de metabolismo quimiolitoautotrófico y quimioheterotrófico, respectivamente, tuvieron mayor actividad de la enzima arsenito oxidasa. Las condiciones óptimas de crecimiento y la actividad enzimática de dichas cepas se investigaron. La cepa 04-SP1qa presentó actividad enzimática específica de 0.162 μmol·min ·mg -1 a pH 7.0 y 40 °C. Los resultados demostraron la presencia de bacterias oxidantes de arsenito con actividad enzimática en suelos de la Comarca Lagunera, identificando potencial para desarrollar nuevas tecnologías de biorremediación de aguas y suelos contaminados con arsénico en la región. Palabras clave:Arsenito oxidasa, quimiolitoautotrófico, quimioheterotrófico, biorremediación.

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Microplate screening assay for the detection of arsenite-oxidizing and arsenate-reducing bacteria

Cited by 43 publications

References 9 publications

Isolation and characterization of an aerobic bacterial consortium able to degrade roxarsone

Isolation and characterization of an aerobic bacterial consortium able to degrade roxarsone

A rapid colony screening method for the detection of arsenate-reducing bacteria

Bioprospección de bacterias oxidantes de arsenito de suelo de la Comarca Lagunera

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