Isolation and characterization of an aerobic bacterial consortium able to degrade roxarsone

Guzmán‐Fierro, Víctor; Moraga, Rubén; León, Carla; Campos, Víctor; Smith, Carlos T.; Mondaca, M. A.

doi:10.1007/s13762-014-0512-4

Cited by 32 publications

(19 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it can be concluded that the key factor influencing the number of cells in the microcosms is the availability of nutrients and not roxarsone. Similar results were reported by Guzmán-Fierro et al (2014) in samples of agricultural soil fertilized with chicken manure feed roxarsone.…”

Section: Discussionsupporting

confidence: 89%

“…strain from chicken litter was able to degrade 100 % of roxarsone in 9 days (Stolz et al 2007) and that it can be biotransformed into inorganic arsenic . So far, there is only one report describing roxarsone biotransformation under aerobic conditions (Guzmán-Fierro et al 2014). Since, as reported by Shi et al (2014) and Stolz et al (2007), roxarsone can act as terminal electron acceptor in microbial respiration, microcosms cultured under anaerobic condition can be better biotransformers of roxarsone.…”

Section: Discussionmentioning

confidence: 99%

“…Aliquots were filtered (0.22 lm Millipore) and 200 lL of each aliquot were transferred, in triplicate, to 96-wells plates. Roxarsone degradation was quantified, using a microplate spectrophotometer (Epoch, BIOTEK), by means of spectrograms integration (310-500 nm) by the trapezoid method, using the Gen5 software (BIOTEK) (Guzmán-Fierro et al 2014).…”

Section: Evaluation Of Roxarsone Biodegradation In Microcosmsmentioning

confidence: 99%

See 2 more Smart Citations

Biodegradation of roxarsone by a bacterial community of underground water and its toxic impact

Mafla

Moraga

León

et al. 2015

World J Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Roxarsone is included in chicken food as anticoccidial and mainly excreted unchanged in faeces. Microorganisms biotransform roxarsone into toxic compounds that leach and contaminate underground waters used for human consumption. This study evaluated roxarsone biotransformation by underground water microorganisms and the toxicity of the resulting compounds. Underground water from an agricultural field was used to prepare microcosms, containing 0.05 mM roxarsone, and cultured under aerobic or anaerobic conditions. Bacterial communities of microcosms were characterized by PCR-DGGE. Roxarsone degradation was measured by HPLC/HG/AAS. Toxicity was evaluated using HUVEC cells and the Toxi-ChromoTest kit. Roxarsone degradation analysis, after 15 days, showed that microcosms of underground water with nutrients degraded 90 and 83.3% of roxarsone under anaerobic and aerobic conditions, respectively. Microcosms without nutrients degraded 50 and 33.1% under anaerobic and aerobic conditions, respectively. Microcosms including nutrients showed more roxarsone conversion into toxic inorganic arsenic species. DGGE analyses showed the presence of Proteobacteria, Firmicutes, Actinobacteria, Planctomycetes and Spirochaetes. Toxicity assays showed that roxarsone biotransformation by underground water microorganisms in all microcosms generated degradation products toxic for eukaryotic and prokaryotic cells. Furthermore, toxicity increased when roxarsone leached though a soil column and was further transformed by the bacterial community present in underground water. Therefore, using underground water from areas where roxarsone containing manure is used as fertilizer might be a health risk.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Evaluation Of Roxarsone Biodegradation In Microcosmsmentioning

confidence: 99%

See 1 more Smart Citation

Biodegradation of roxarsone by a bacterial community of underground water and its toxic impact

Mafla

Moraga

León

et al. 2015

World J Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Data was analysed using two-way ANOVA and Student’s T test using the GraphPad Prism 5 software. P values <0.05 were considered as statistically significant [ 51 ]. Associations between variables were calculated by Pearson’s correlation.…”

Section: Methodsmentioning

confidence: 99%

Effect of the natural arsenic gradient on the diversity and arsenic resistance of bacterial communities of the sediments of Camarones River (Atacama Desert, Chile)

et al. 2018

View full text Add to dashboard Cite

Arsenic (As), a highly toxic metalloid, naturally present in Camarones River (Atacama Desert, Chile) is a great health concern for the local population and authorities. In this study, the taxonomic and functional characterization of bacterial communities associated to metal-rich sediments from three sites of the river (sites M1, M2 and M3), showing different arsenic concentrations, were evaluated using a combination of approaches. Diversity of bacterial communities was evaluated by Illumina sequencing. Strains resistant to arsenic concentrations varying from 0.5 to 100 mM arsenite or arsenate were isolated and the presence of genes coding for enzymes involved in arsenic oxidation (aio) or reduction (arsC) investigated. Bacterial communities showed a moderate diversity which increased as arsenic concentrations decreased along the river. Sequences of the dominant taxonomic groups (abundances ≥1%) present in all three sites were affiliated to Proteobacteria (range 40.3–47.2%), Firmicutes (8.4–24.8%), Acidobacteria (10.4–17.1%), Actinobacteria (5.4–8.1%), Chloroflexi (3.9–7.5%), Planctomycetes (1.2–5.3%), Gemmatimonadetes (1.2–1.5%), and Nitrospirae (1.1–1.2%). Bacterial communities from sites M2 and M3 showed no significant differences in diversity between each other (p = 0.9753) but they were significantly more diverse than M1 (p<0.001 and p<0.001, respectively). Sequences affiliated with Proteobacteria, Firmicutes, Acidobacteria, Chloroflexi and Actinobacteria at M1 accounted for more than 89% of the total classified bacterial sequences present but these phyla were present in lesser proportions in M2 and M3 sites. Strains isolated from the sediment of sample M1, having the greatest arsenic concentration (498 mg kg-1), showed the largest percentages of arsenic oxidation and reduction. Genes aio were more frequently detected in isolates from M1 (54%), whereas arsC genes were present in almost all isolates from all three sediments, suggesting that bacterial communities play an important role in the arsenic biogeochemical cycle and detoxification of arsenical compounds. Overall, results provide further knowledge on the microbial diversity of arsenic contaminated fresh-water sediments.

show abstract

“…Recently, a new metabolite of roxarsone, N-acetyl-4-hydroxy-m-arsanilic acid (N-AHAA), was identified (Yang et al 2016). These arsenical products can then contaminate soil, groundwater, and surface water (Morrison 1969; D r a f t 3 Mafla et al 2015;Guzmán-Fierro et al 2015), and the degradation process of roxarsone is greatly influenced by environmental factors such as temperature, humidity, and organic content (Sun 2012). However, the degradation rate and microbial community structure under different culture environment, such as aerobic, various lighting, and anaerobic conditions, have never been compared together.…”

mentioning

confidence: 99%

Response of microbial communities to roxarsone under different culture conditions

Liu

Zhang

et al. 2017

Can. J. Microbiol.

View full text Add to dashboard Cite

Roxarsone is a feed additive widely used in the broiler and swine industries that has the potential to contaminate the environment, mainly via the use of poultry manure as fertilizer, which results in release of inorganic arsenic to the soil and water. This study was conducted to investigate roxarsone degradation and the response of the microbial community under different culture conditions using high-throughput sequencing technology. Poultry litter was incubated for 288 h in the presence of roxarsone under light aerobic, dark aerobic, or dark anaerobic conditions. The results showed that roxarsone was completely degraded after 48 h of dark anaerobic incubation, while 79.9% and 94.5% of roxarsone was degraded after 288 h of dark aerobic and light aerobic incubation, respectively. Under dark aerobic conditions with microbial inhibitor sodium azide, roxarsone was rarely degraded during the 288 h of incubation, illustrating that microorganisms play an important role in roxarsone degradation. Microbial community structure was significantly different among various culture conditions. Olivibacter, Sphingobacterium, and Proteiniphilum were the top 3 genera in the control samples. Sphingobacterium and Alishewanella dominated the light aerobic samples, while the dominant microflora of the dark aerobic samples were Acinetobacter spp. Pseudomonas and Advenella were the predominant genera of dark anaerobic samples. This study emphasizes the potential importance of microbes in roxarsone degradation and expands our current understanding of microbial ecology during roxarsone degradation under different environmental conditions.

show abstract

Isolation and characterization of an aerobic bacterial consortium able to degrade roxarsone

Cited by 32 publications

References 45 publications

Biodegradation of roxarsone by a bacterial community of underground water and its toxic impact

Biodegradation of roxarsone by a bacterial community of underground water and its toxic impact

Effect of the natural arsenic gradient on the diversity and arsenic resistance of bacterial communities of the sediments of Camarones River (Atacama Desert, Chile)

Response of microbial communities to roxarsone under different culture conditions

Contact Info

Product

Resources

About