Micropillar substrates: A tool for studying cell mechanobiology

Gupta, Mayank; Kocgozlu, Leyla; Sarangi, Bibhu Ranjan; Margadant, Felix; Ashraf, Mohammed; Ladoux, Benoît

doi:10.1016/bs.mcb.2014.10.009

Cited by 52 publications

(51 citation statements)

References 36 publications

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“…To measure the forces exerted through cellular functions such as adhesion, migration, and proliferation, micropatterned substrates including micropillar substrates are frequently used. 9,10 However, it is known that the substrate topography causes a certain effect on the cellular functions. 11,12 Nanomechanical sensors, on the other hand, can reduce such problems and might provide a new sensing platform with a plane surface.…”

Section: Introductionmentioning

confidence: 99%

Finite Element Analysis on Nanomechanical Sensing of Cellular Forces

2016

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Detection of cellular forces plays an important role in investigating the mechanical basis of cells. As nanomechanical sensors can directly detect surface stress, they can be utilized to detect cellular forces. In the present study, we perform quantitative simulations of nanomechanical sensors for the detection of cellular forces using finite element analyses (FEA). We focus on two types of nanomechanical sensors: a cantilever-type sensor and a membrane-type surface stress sensor (MSS). It is found that sensing signals can be obtained when cells on the nanomechanical sensors synchronize their motions. To effectively detect cellular forces on the nanomechanical sensors, we discuss the optimization scheme for a coating layer on the surface of the sensors.

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Section: Introductionmentioning

confidence: 99%

Finite Element Analysis on Nanomechanical Sensing of Cellular Forces

2016

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“…[1,4,6,7] By coating the pillar tops with adhesive ligands and tuning pillar stiffness to cell forces, such arrays are commonly used measure cellular traction forces. [8,9] We have previously adapted pillar arrays for the investigation of small motile cells that can be placed between the pillars. [10] In these arrays, the uniform pillars do not serve as substrates for cell adhesion but as obstacles for the migrating cells, which can be different types of eukaryotic or bacterial cells.…”

Section: Introductionmentioning

confidence: 99%

Microstructured Blood Vessel Surrogates Reveal Structural Tropism of Motile Malaria Parasites

Muthinja

Ripp

Hellmann

et al. 2017

Adv Healthcare Materials

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Muthinja, M. J. et al. (2017) Microstructured blood vessel surrogates reveal structural tropism of motile malaria parasites. Advanced Healthcare Materials, 6(6), 1601178. (doi:10.1002/adhm.201601178) There may be differences between this version and the published version. You are advised to consult the publisher's version if you wish to cite from it. This is the peer-reviewed version of the following article: Muthinja, M. J. et al. (2017) Investigating the association of sporozoites to pillars in arrays displaying pillars of different diameters revealed that the crescent-shaped parasites prefer to associate with and migrate around pillars with a similar curvature. This suggests that after transmission by a mosquito malaria parasites might use a structural tropism to recognize blood capillaries in the dermis in order to gain access to the blood stream.4

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“…Several excellent reviews [21,20,22,23] have summarized the role of traction forces in various biological processes and the corresponding tools available to quantify them. Here, we focus specifically on the advancement of traction force microscopy (TFM) and microfabricated tissues for quantifying traction forces in the context of cell migration and tissue morphogenesis.…”

Section: Introductionmentioning

confidence: 99%

Microfabricated tissues for investigating traction forces involved in cell migration and tissue morphogenesis

Nerger

Siedlik

Nelson

2016

Cell. Mol. Life Sci.

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Cell-generated forces drive an array of biological processes ranging from wound healing to tumor metastasis. Whereas experimental techniques such as traction force microscopy are capable of quantifying traction forces in multidimensional systems, the physical mechanisms by which these forces induce changes in tissue form remain to be elucidated. Understanding these mechanisms will ultimately require techniques that are capable of quantifying traction forces with high precision and accuracy in vivo or in systems that recapitulate in vivo conditions, such as microfabricated tissues and engineered substrata. To that end, here we review the fundamentals of traction forces, their quantification, and the use of microfabricated tissues designed to study these forces during cell migration and tissue morphogenesis. We emphasize the differences between traction forces in two- and three-dimensional systems, and highlight recently developed techniques for quantifying traction forces.

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Micropillar substrates: A tool for studying cell mechanobiology

Cited by 52 publications

References 36 publications

Finite Element Analysis on Nanomechanical Sensing of Cellular Forces

Finite Element Analysis on Nanomechanical Sensing of Cellular Forces

Microstructured Blood Vessel Surrogates Reveal Structural Tropism of Motile Malaria Parasites

Microfabricated tissues for investigating traction forces involved in cell migration and tissue morphogenesis

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