Microphenomics for Interactions of Barley with Fungal Pathogens

Douchkov, Dimitar; Baum, Tobias; Ihlow, Alexander; Schweizer, Patrick; Seiffert, Udo

doi:10.1007/978-94-007-7575-6_5

Cited by 12 publications

(9 citation statements)

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“…All RILs, the parental lines, and the susceptible standard 'Borenos' were evaluated for resistance at seedling stage in a detached leaf assay (Douchkov et al 2012). Seedlings were grown in 77-cell trays with mixed potting soil (Gebr.…”

Section: Phenotypic Assessment Of Leaf Rust Resistance In Seedlingsmentioning

confidence: 99%

QTL mapping of adult plant and seedling resistance to leaf rust (Puccinia triticina Eriks.) in a multiparent advanced generation intercross (MAGIC) wheat population

et al. 2020

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Key message The Bavarian MAGIC Wheat population, comprising 394 F6:8 recombinant inbred lines was phenotyped for Puccinia triticina resistance in multi-years’ field trials at three locations and in a controlled environment seedling test. Simple intervall mapping revealed 19 QTL, corresponding to 11 distinct chromosomal regions. Abstract The biotrophic rust fungus Puccinia triticina is one of the most important wheat pathogens with the potential to cause yield losses up to 70%. Growing resistant cultivars is the most cost-effective and environmentally friendly way to encounter this problem. The emergence of leaf rust races being virulent against common resistance genes increases the demand for wheat varieties with novel resistances. In the past decade, the use of complex experimental populations, like multiparent advanced generation intercross (MAGIC) populations, has risen and offers great advantages for mapping resistances. The genetic diversity of multiple parents, which has been recombined over several generations, leads to a broad phenotypic diversity, suitable for high-resolution mapping of quantitative traits. In this study, interval mapping was performed to map quantitative trait loci (QTL) for leaf rust resistance in the Bavarian MAGIC Wheat population, comprising 394 F6:8 recombinant inbred lines (RILs). Phenotypic evaluation of the RILs for adult plant resistance was carried out in field trials at three locations and two years, as well as in a controlled-environment seedling inoculation test. In total, interval mapping revealed 19 QTL, which corresponded to 11 distinct chromosomal regions controlling leaf rust resistance. Six of these regions may represent putative new QTL. Due to the elite parental material, RILs identified to be resistant to leaf rust can be easily introduced in breeding programs.

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Section: Phenotypic Assessment Of Leaf Rust Resistance In Seedlingsmentioning

confidence: 99%

QTL mapping of adult plant and seedling resistance to leaf rust (Puccinia triticina Eriks.) in a multiparent advanced generation intercross (MAGIC) wheat population

et al. 2020

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“…hordei (Bgh) [15] that also fulfills several criteria of a model plantpathogen interaction due to a large body of physiological, cellular, biochemical, and molecular information on changes in the host during compatible or resistant interactions [16][17][18]. Transient expression and genesilencing assays such as transient-induced gene silencing (TIGS) in bombarded epidermal cells have been developed over the years and proven to be valuable tools for a better understanding of barley/powdery mildew interactions [19][20][21]. NHR of barley against non-adapted formae speciales or species of powdery mildew such as the wheat pathogen B. graminis f.sp.…”

Section: Introductionmentioning

confidence: 99%

“…By using TIGS we screened three groups of host genes that were: (1) previously found to be upregulated in powdery mildew-attacked barley epidermal cells [19,21,25,26]; (2) belonging to selected multigene-families; or (3) localizing within the confidence interval of a meta-QTL for resistance to Bgh on chromosome 5H [9,27]. The data of QR modulation by RNAi constructs targeting the corresponding transcripts were combined with meta-data of transcript regulation, SNP or gene haplotype associations with QR to Bgh, co-localization of the RNAi target genes with QTL for resistance to Bgh, and copy number variation.…”

Section: Introductionmentioning

confidence: 99%

Discovery of genes affecting resistance of barley to adapted and non-adapted powdery mildew fungi

Douchkov

Lück

Johrde³

et al. 2014

Genome Biol

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Background: Non-host resistance, NHR, to non-adapted pathogens and quantitative host resistance, QR, confer durable protection to plants and are important for securing yield in a longer perspective. However, a more targeted exploitation of the trait usually possessing a complex mode of inheritance by many quantitative trait loci, QTLs, will require a better understanding of the most important genes and alleles.

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“…hordei ( Bgh ) [ 15 ] that also fulfills several criteria of a model plant-pathogen interaction due to a large body of physiological, cellular, biochemical, and molecular information on changes in the host during compatible or resistant interactions [ 16 - 18 ]. Transient expression and gene-silencing assays such as transient-induced gene silencing (TIGS) in bombarded epidermal cells have been developed over the years and proven to be valuable tools for a better understanding of barley/powdery mildew interactions [ 19 - 21 ]. NHR of barley against non-adapted formae speciales or species of powdery mildew such as the wheat pathogen B. graminis f.sp.…”

Section: Introductionmentioning

confidence: 99%

“…Here we describe a large-scale functional approach in barley for the identification of genes that are relevant for NHR and QR to Bgt and Bgh , respectively [ 23 ]. By using TIGS we screened three groups of host genes that were: (1) previously found to be upregulated in powdery mildew-attacked barley epidermal cells [ 19 , 21 , 25 , 26 ]; (2) belonging to selected multigene-families; or (3) localizing within the confidence interval of a meta-QTL for resistance to Bgh on chromosome 5H [ 9 , 27 ]. The data of QR modulation by RNAi constructs targeting the corresponding transcripts were combined with meta-data of transcript regulation, SNP or gene haplotype associations with QR to Bgh , co-localization of the RNAi target genes with QTL for resistance to Bgh , and copy number variation.…”

Section: Introductionmentioning

confidence: 99%

Discovery of genes affecting resistance of barley to adapted and non-adapted powdery mildew fungi

Douchkov

Lück

Johrde³

et al. 2014

Genome Biol

Self Cite

View full text Add to dashboard Cite

BackgroundNon-host resistance, NHR, to non-adapted pathogens and quantitative host resistance, QR, confer durable protection to plants and are important for securing yield in a longer perspective. However, a more targeted exploitation of the trait usually possessing a complex mode of inheritance by many quantitative trait loci, QTLs, will require a better understanding of the most important genes and alleles.ResultsHere we present results from a transient-induced gene silencing, TIGS, approach of candidate genes for NHR and QR in barley against the powdery mildew fungus Blumeria graminis. Genes were selected based on transcript regulation, multigene-family membership or genetic map position. Out of 1,144 tested RNAi-target genes, 96 significantly affected resistance to the non-adapted wheat- or the compatible barley powdery mildew fungus, with an overlap of four genes. TIGS results for QR were combined with transcript regulation data, allele-trait associations, QTL co-localization and copy number variation resulting in a meta-dataset of 51 strong candidate genes with convergent evidence for a role in QR.ConclusionsThis study represents an initial, functional inventory of approximately 3% of the barley transcriptome for a role in NHR or QR against the powdery mildew pathogen. The discovered candidate genes support the idea that QR in this Triticeae host is primarily based on pathogen-associated molecular pattern-triggered immunity, which is compromised by effector molecules produced by the compatible pathogen. The overlap of four genes with significant TIGS effects both in the NHR and QR screens also indicates shared components for both forms of durable pathogen resistance.Electronic supplementary materialThe online version of this article (doi:10.1186/s13059-014-0518-8) contains supplementary material, which is available to authorized users.

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Microphenomics for Interactions of Barley with Fungal Pathogens

Cited by 12 publications

References 84 publications

QTL mapping of adult plant and seedling resistance to leaf rust (Puccinia triticina Eriks.) in a multiparent advanced generation intercross (MAGIC) wheat population

QTL mapping of adult plant and seedling resistance to leaf rust (Puccinia triticina Eriks.) in a multiparent advanced generation intercross (MAGIC) wheat population

Discovery of genes affecting resistance of barley to adapted and non-adapted powdery mildew fungi

Discovery of genes affecting resistance of barley to adapted and non-adapted powdery mildew fungi

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