Microperoxidase-11 Immobilized in a Metal Organic Framework

Engineering Metal Organic Frameworks for Heterogeneous Catalysis

“…Balkus Jr. and co-workers have used 3-dimensional [Cu(OOC-C 6 H 4 -C 6 H 4 -COO) 0.5 C 6 H 12 N 2 ] n MOF to immobilize a microperoxidase that is able to act as a heterogeneous biocatalyst and effect the oxidation of a-methylstyrene as well as methylene blue. 136 2.2.3.3. Alcohols to Aldehydes and Ketones.…”

Metal organic frameworks mimicking natural enzymes: a structural and functional analogy

“…[124][125][126][127][128] Although currently theoretical, construction of artificial enzymes is an enticing possibility; only a handful of successful paradigms properly functioning under ambient conditions have been synthesized. [143][144][145][146][147] In spite of viable opportunities, the growth of biomimetic MOFs is, however, quite slow compared to the gradually maturing MOF chemistry (Fig. MOFs are envisioned as the most appropriate candidate for this purpose because their extremely ordered tailorable cavities or channels are susceptible to provide a hydrophobic confined environment akin to natural enzymes as well as offer an ordered distribution of highly dense substrate accessible active catalytic sites throughout the architecture.…”

“…Customization of MOFs also offers enough room to encapsulate enzyme molecules into MOF cages by utilizing proper size correlations. [143][144][145][146][147] In spite of viable opportunities, the growth of biomimetic MOFs is, however, quite slow compared to the gradually maturing MOF chemistry (Fig. 1).…”

Significant Enhancement of Structural Stability of the Hyperhalophilic ADH fromHaloferax volcaniivia Entrapment on Metal Organic Framework Support

“…20,31 While the microporous nature of MOFs makes it difficult to incorporate large macromolecules such as enzymes, recent studies have demonstrated that proteins can be retained within the cavities of some MOFs. [32][33][34] A number of reports have focused on developing a general immobilization methodology to encapsulate an enzyme in one simple step. 30,[35][36] Many studies focused on the in-situ encapsulation of enzymes in aqueous system in materials such as zeolite-like structures.…”

“…Previous work demonstrated that HvADH2 can be covalently attached to polymeric beads . A wide range of supports can be used for enzyme immobilization, including natural and synthetic polymers, and inorganic porous materials. − The use of metal organic framework materials (MOF) as supports for the immobilization of enzymes has been described recently. − MOF materials have been used for water adsorption and gas storage, for separation, sensing, heterogeneous catalysis, nanoreactors, and drug delivery among others. , The wide range of MOF structures arises from their topological and compositional versatility. , While the microporous nature of MOFs makes it difficult to incorporate large macromolecules such as enzymes, recent studies have demonstrated that proteins can be retained within the cavities of some MOFs. − A number of reports have focused on developing a general immobilization methodology to encapsulate an enzyme in one simple step. ,, Many studies focused on the in situ encapsulation of enzymes in aqueous system in materials such as zeolite-like structures. − Our recent work demonstrated that it is possible to encapsulate a range of enzymes (including alcohol dehydrogenase from Saccharomyces cerevisiae ) in a facile manner using a Fe-BTC MOF material that is structurally similar to commercially available Basolite F300. − With this approach, unlike other immobilization methods enzyme immobilization occurs in situ, at mild pH, and at room temperature in a rapid (10 min) and facile manner. In this work, we describe the in situ immobilization of the halophlic HvADH2 in the Fe-BTC MOF.…”