Micropatterning of endothelial cells to create a capillary-like network with defined architecture by laser-assisted bioprinting

Kérourédan, Olivia; Bourget, Jean-Michel; Rémy, Murielle; Crauste–Manciet, Sylvie; Kalisky, Jérôme; Catros, Sylvain; Thebaud, Noëlie; Devillard, Raphaël

doi:10.1007/s10856-019-6230-1

Cited by 71 publications

(67 citation statements)

References 49 publications

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“…The cell droplets were deposited as lines in a first approach, and then deposited to form circles. The formation of a capillary-like network that grew following the induced pattern was visible at Day 6 (line) and Day 3 (circles) (Figure 9d) [42]. Previous works showed the capability to print cells in situ using LAB bioprinting [135,136].…”

Section: Bioprinting Techniquesmentioning

confidence: 91%

“…During culture, spheroids were able to fuse and form ring-like and tube-like vascular constructs but with an internal diameter reduction of 50% after spheroid fusion and tissue formation. In a recent study, ECs labeled with fluorescent protein dTomato were patterned via laser-assisted bioprinting onto a collagen biopaper with embedded mesenchymal stem cells (MSCs) [42]. High EC density allowed the development of a stable and connected network but the deposition of VEGF and hMSCs onto the collagen biopaper improved formation and stability.…”

Section: Materials For Bioprintingmentioning

confidence: 99%

“…( d ) Laser-assisted bioprinting (LAB) technology (left) to optimize the bioprinting of HUVEC lines (middle) and their maturation into a pre-oriented micro-vascular network after six days of culture (right). Reprinted by permission from Copyright Clearance Center: Springer Nature, Journal of Materials Science: Materials in Medicine, Micropatterning of endothelial cells to create a capillary-like network with defined architecture by laser-assisted bioprinting, Olivia Kérourédan, Jean-Michel Bourget, Murielle Rémy et al, 2019 [42].…”

Section: Figurementioning

confidence: 99%

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Bioprinting Vasculature: Materials, Cells and Emergent Techniques

et al. 2019

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Despite the great advances that the tissue engineering field has experienced over the last two decades, the amount of in vitro engineered tissues that have reached a stage of clinical trial is limited. While many challenges are still to be overcome, the lack of vascularization represents a major milestone if tissues bigger than approximately 200 µm are to be transplanted. Cell survival and homeostasis is to a large extent conditioned by the oxygen and nutrient transport (as well as waste removal) by blood vessels on their proximity and spontaneous vascularization in vivo is a relatively slow process, leading all together to necrosis of implanted tissues. Thus, in vitro vascularization appears to be a requirement for the advancement of the field. One of the main approaches to this end is the formation of vascular templates that will develop in vitro together with the targeted engineered tissue. Bioprinting, a fast and reliable method for the deposition of cells and materials on a precise manner, appears as an excellent fabrication technique. In this review, we provide a comprehensive background to the fields of vascularization and bioprinting, providing details on the current strategies, cell sources, materials and outcomes of these studies.

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Section: Bioprinting Techniquesmentioning

confidence: 91%

Section: Materials For Bioprintingmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Bioprinting Vasculature: Materials, Cells and Emergent Techniques

et al. 2019

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“…In addition, they also demonstrated that the procedure of laser-assisted bioprinting did not affected the proliferation ability and differentiation behavior of the hASCs. In another study, ECs were bioprinted by laser-assisted bioprinting technology onto a collagen hydrogel scaffold previously seeded with mesenchymal stem cells (MSCs) to fabricate a microvascular network [93]. Kérourédan et al utilized laser-assisted bioprinting to bioprint ECs in situ into mouse calvarial bone defects prefilled with collagen scaffold containing MSCs and vascular endothelial growth factor (VEGF) [94].…”

Section: Laser-assisted Bioprintingmentioning

confidence: 99%

3D Bioprinting for Vascularized Tissue-Engineered Bone Fabrication

et al. 2020

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Vascularization in bone tissues is essential for the distribution of nutrients and oxygen, as well as the removal of waste products. Fabrication of tissue-engineered bone constructs with functional vascular networks has great potential for biomimicking nature bone tissue in vitro and enhancing bone regeneration in vivo. Over the past decades, many approaches have been applied to fabricate biomimetic vascularized tissue-engineered bone constructs. However, traditional tissue-engineered methods based on seeding cells into scaffolds are unable to control the spatial architecture and the encapsulated cell distribution precisely, which posed a significant challenge in constructing complex vascularized bone tissues with precise biomimetic properties. In recent years, as a pioneering technology, three-dimensional (3D) bioprinting technology has been applied to fabricate multiscale, biomimetic, multi-cellular tissues with a highly complex tissue microenvironment through layer-by-layer printing. This review discussed the application of 3D bioprinting technology in the vascularized tissue-engineered bone fabrication, where the current status and unique challenges were critically reviewed. Furthermore, the mechanisms of vascular formation, the process of 3D bioprinting, and the current development of bioink properties were also discussed.

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“…60,[75][76][77] The limitations of this technology are high cost, long printing time, and potential metal infection risk due to evaporation of the metal absorbing layer during printing and applying the laser beam. 78 Kérourédan et al 79 applied the laserassisted bioprinter to coculture tdTomato-labeled endothelial cells with MSCs seeded on collagen hydrogels. The results indicated that the formation of capillary-like structures was related to the concentration of local endothelial cells.…”

Section: Laser-assisted Bioprintingmentioning

confidence: 99%

Bioprinting a cell‐laden matrix for bone regeneration: A focused review

Ghorbani

Zhong

et al. 2020

J of Applied Polymer Sci

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Although many efforts have been made to regenerate the bone lesions, existing challenges can be mitigated through the development of tissue engineering scaffolds. However, the weak control on the microstructure of constructs, limitation in preparation of patient-specific and multilayered scaffolds, restriction in the fabrication of cell-laden matrixes, and challenges in preserving the drug/growth factors' efficacy in conventional methods have led to the development of bioprinting technology for regeneration of bone defects. So in this review, conventional 3D printers are classified, then the priority of the different types of bioprinting technologies for the preparation of the cell/growth factor-laden matrixes are focused. Besides, the bio-ink compositions, including polymeric/hybrid hydrogels and cell-based bio-inks are classified according to fundamental and recent studies. Herein, different effective parameters, such as viscosity, rheological properties, cross-linking methods, biodegradation biocompatibility, are considered. Finally, different types of cells and growth factors that can encapsulate in the bio-inks to promote bone repair are discussed, and both in vitro and in vivo achievement are considered. This review provides current and future perspectives of cell-laden bioprinting technologies. The restrictions and challenges are identified, and proper strategies for the development of cell-laden matrixes and high-performance printable bio-inks are proposed.

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Micropatterning of endothelial cells to create a capillary-like network with defined architecture by laser-assisted bioprinting

Cited by 71 publications

References 49 publications

Bioprinting Vasculature: Materials, Cells and Emergent Techniques

Bioprinting Vasculature: Materials, Cells and Emergent Techniques

3D Bioprinting for Vascularized Tissue-Engineered Bone Fabrication

Bioprinting a cell‐laden matrix for bone regeneration: A focused review

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