Micropatterned Fibrous Scaffolds Fabricated Using Electrospinning and Hydrogel Lithography: New Platforms to Create Cellular Micropatterns

Lee, Hyun Jong; Nam, Seung Hee; Son, Kyung Jin; Koh, Won Gun

doi:10.1016/j.snb.2010.05.032

Cited by 19 publications

(13 citation statements)

References 32 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Modifications to the physical and chemical structures of these workhorse polymers have expanded their use to a certain degree. In addition to the macroscopic scale polymers produced via thermal and injection molding, polymeric nanofibers generated via an electrospinning process have been utilized in protein assays to provide an increased surface area for protein assembly [ 92 , 93 ]. The chemical heterogeneity of polymers employed in protein measurements has also been altered by several means in order to localize proteins to certain areas and to incorporate specific chemical properties into these areas.…”

Section: Polymers Currently Used In Analytical Detection Of Proteinsmentioning

confidence: 99%

Functional Polymers in Protein Detection Platforms: Optical, Electrochemical, Electrical, Mass-Sensitive, and Magnetic Biosensors

Hahm

2011

Sensors

View full text Add to dashboard Cite

The rapidly growing field of proteomics and related applied sectors in the life sciences demands convenient methodologies for detecting and measuring the levels of specific proteins as well as for screening and analyzing for interacting protein systems. Materials utilized for such protein detection and measurement platforms should meet particular specifications which include ease-of-mass manufacture, biological stability, chemical functionality, cost effectiveness, and portability. Polymers can satisfy many of these requirements and are often considered as choice materials in various biological detection platforms. Therefore, tremendous research efforts have been made for developing new polymers both in macroscopic and nanoscopic length scales as well as applying existing polymeric materials for protein measurements. In this review article, both conventional and alternative techniques for protein detection are overviewed while focusing on the use of various polymeric materials in different protein sensing technologies. Among many available detection mechanisms, most common approaches such as optical, electrochemical, electrical, mass-sensitive, and magnetic methods are comprehensively discussed in this article. Desired properties of polymers exploited for each type of protein detection approach are summarized. Current challenges associated with the application of polymeric materials are examined in each protein detection category. Difficulties facing both quantitative and qualitative protein measurements are also identified. The latest efforts on the development and evaluation of nanoscale polymeric systems for improved protein detection are also discussed from the standpoint of quantitative and qualitative measurements. Finally, future research directions towards further advancements in the field are considered.

show abstract

Section: Polymers Currently Used In Analytical Detection Of Proteinsmentioning

confidence: 99%

Functional Polymers in Protein Detection Platforms: Optical, Electrochemical, Electrical, Mass-Sensitive, and Magnetic Biosensors

Hahm

2011

Sensors

View full text Add to dashboard Cite

show abstract

“…Fabrication of micropatterned PS/PSMA fibers Micropatterned polymeric nanofibers were prepared by combining PEG hydrogel lithography with an electrospinning technique. 32 To produce polymeric nanofibers, a mixture of PS and PSMA was electrospun instead of using only PS, which is the most common polymer for electrospinning. The use of the PSMA copolymer with PS makes it easier to attach proteins onto nanofibers without using complicated chemical modification steps because the PSMA copolymer contains a functional group of maleic anhydride (MA) that can readily form covalent bonds with primary amines on the proteins.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of hydrogel-micropatterned nanofibers for highly sensitive microarray-based immunosensors having additional enzyme-based sensing capability

Lee

Son

et al. 2011

J. Mater. Chem.

Self Cite

View full text Add to dashboard Cite

Nanofiber-based protein microarrays were fabricated through a combination of electrospinning and hydrogel lithography. Electrospinning generated polystyrene (PS)/poly(styrene-alt-maleic anhydride) (PSMA) fibers with diameters ranging from 0.5 to 1.0 mm and photopatterning of poly(ethylene glycol) (PEG) hydrogel on the electrospun fibers created clearly defined hydrogel microstructures with incorporated nanofibers. The resultant micropatterned nanofibrous substrates were obtained as freestanding and bidirectionally porous sheets, where most of the nanofibers were inserted through the side walls of the hydrogel microstructures. Because of the protein-repellent nature of PEG hydrogels, IgG was selectively immobilized only within the nanofibrous region, creating an IgG microarray. Due to increased surface area, IgG loading in nanofibrous substrates was about six times greater than on planar substrates, which consequently yielded a higher fluorescence signal and faster reaction rate in immunoassays. The capability of encapsulating enzymes made it possible for PEG hydrogels to be used not only for defining protein micropatterns but also for additional biosensor elements. Based on this result, micropatterned nanofibrous substrates consisting of IgG-immobilized nanofibers and enzymeentrapping PEG hydrogels were fabricated, and their potential to simultaneously carry out both immunoassays and enzyme-based assays was successfully demonstrated.

show abstract

“…Currently, traditional lithographic techniques can be used to localize nanofiber structures 11. However, they usually require very complicated fabrication procedures, which is labor intensive and time consuming.…”

Section: Methodsmentioning

confidence: 99%

Simple Localization of Nanofiber Scaffolds via SU‐8 Photoresist and Their Use for Parallel 3D Cellular Assays

Jiang¹,

Zhang²,

Li³

et al. 2012

Advanced Materials

View full text Add to dashboard Cite

Nanofi ber scaffolds made from synthetic or natural polymers have shown promise for biological applications because of their extremely high surface-to-mass ratio, porous structures with excellent pore interconnectivity, functionalities, and surface chemistry of the polymers. [1][2][3][4] The nanometer-scale structure of the extracellular matrix (ECM) in vivo is a natural assembly of intricate nanofi bers, including protein fi brils and fi bers for cell support, and acts as an instructive background to guide cell behavior. Owing to the similarity of the fi brous structure of electrospun nanofi bers to that of the natural ECM, many attempts have been made to fabricate nanofi ber scaffolds to mimic the chemical composition and structural properties of the ECM and study cell-ECM interactions under biophysical and biochemical stimuli in in vivo environments. [5][6][7] Moreover, several techniques have been developed to produce different nanofi ber features, such as the alignment and organization of nanofi bers by means of an electric fi eld or by applying mechanical force; [8][9][10] however, little effort has been devoted to producing nanofi ber scaffolds with the desired features in a simple way in order to realize multiplex bioassays and for advanced biological applications.Currently, traditional lithographic techniques can be used to localize nanofi ber structures. [ 11 ] However, they usually require very complicated fabrication procedures, which is labor intensive and time consuming. In particular, the deposition of residual chemical agents might potentially affect the properties of the materials because of the internal porous structure and fragile nature of nanofi bers, thus limiting their practical utilization. UV laser ablation is a method chosen to fabricate nanofi bers with various features, [ 12 ] but this process usually takes several hours to degrade the nanofi ber scaffolds before the desired structures are obtained. Furthermore, it is diffi cult to integrate the above methods with microfl uidic components in order to enhance the functionality for advanced applications.In this Communication, we propose a new and facile method to localize nanofi ber scaffolds and produce a high-throughput 3D cell culture array. This approach can be further integrated with microfl uidic devices easily to enhance the functionality for multi ple cellular assays. The method uses 3D biocompatible nanofi ber substrates to mimic in vivo environments and thus facilitates more realistic applications, ranging from stem cell research to faster drug screening and cancer biology applications.Scheme 1 illustrates schematically the procedures used to create a nanofi ber-scaffold cell culture array. Simply, the biocompatible polymer was fi rst electrospun to produce a thin sheet/layer of nanofi bers (ca. 100 μ m thick) on a glass slide drilled with an array of holes (700 μ m in depth). Then, liquid SU-8 2035 negative photoresist was written with a tip on the sheet of nanofi ber scaffolds directly under computer control in order to prod...

show abstract

Micropatterned Fibrous Scaffolds Fabricated Using Electrospinning and Hydrogel Lithography: New Platforms to Create Cellular Micropatterns

Cited by 19 publications

References 32 publications

Functional Polymers in Protein Detection Platforms: Optical, Electrochemical, Electrical, Mass-Sensitive, and Magnetic Biosensors

Functional Polymers in Protein Detection Platforms: Optical, Electrochemical, Electrical, Mass-Sensitive, and Magnetic Biosensors

Fabrication of hydrogel-micropatterned nanofibers for highly sensitive microarray-based immunosensors having additional enzyme-based sensing capability

Simple Localization of Nanofiber Scaffolds via SU‐8 Photoresist and Their Use for Parallel 3D Cellular Assays

Contact Info

Product

Resources

About