Fabrication of hydrogel-micropatterned nanofibers for highly sensitive microarray-based immunosensors having additional enzyme-based sensing capability

Lee, Yeol; Lee, Hyun Jong; Son, Kyung Jin; Koh, Won Gun

doi:10.1039/c0jm03881d

Cited by 46 publications

(24 citation statements)

References 39 publications

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“…In an interesting study, Lee et al (2011) [233] fabricated nanofibre-based protein microarrays through a combination of electrospinning and hydrogel lithography. Clearly defined hydrogel microstructures with incorporated nanofibres were created through electrospinning of polystyrene (PS)/poly(styrene-alt-maleic anhydride) (PSMA) fibres with diameters ranging from 0.5 to 1.0 μm followed by photopatterning of poly(ethylene glycol) (PEG) hydrogel on the electrospun fibres.…”

Section: Immunosensors Through Electrospinningmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

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Biological interactions in biosensors occur through different mechanisms, on the basis of the type of biological receptor elements employed therein that confer the biological specificity to the biosensors themselves (biocatalytic, biocomplexing or bioaffinity biosensors). The use of different transduction systems (amperometric, potentiometric, field-effect transistors, piezometric and conductometric) defines the mode of detection. The interest and relevance of nanomaterials in the fabrication of nanobiosensors lie in their extraordinary properties that make them ideally suited and very promising for sensing applications. The resulting nanobiosensors are capable of sensing analytes in traces with fast, precise and accurate biological identification through miniaturised and easy to use systems. These advanced sensors are also characterised by lower detection limits, higher sensitivity values and high stability, and can further offer multi-detection possibilities. These exceptional features make nanobiosensors as the favourite tools in quality control, food safety, and traceability for their capacity of revealing and warning people against the presence of pathogens, toxins and pollutants, as well as bioterrorism agents. Despite the outstanding properties of these nanobiosensors, however, the efficiency of the biorecognition agent and the number of biorecognition sites available for interacting with target analytes can limit the sensitivity of this kind of sensors. The number of available biorecognition sites is directly related to the surface area of the sensor. Electrospinning technology can significantly increase the sensitivity of biosensors by replacing the typical planar interactive surface of conventional biosensors with a mat of electrospun nonwoven nanofibres, thereby taking advantage of the ultrahigh surface area offered by electrospun nanofibres. Moreover, adjusting the electrospinning process to produce

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Section: Immunosensors Through Electrospinningmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

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“…Recent works have also been devoted to the development of original microarray-based immunoassays using either hydrogel-micropatterned PS-based NFs [ 101 ] or droplets immobilized on polycaprolactone NFs [ 102 ].…”

Section: Electrospun Nfs In Elisa-type Bioassays and Point-of-carementioning

confidence: 99%

Recent Advances in Electrospun Nanofiber Interfaces for Biosensing Devices

Sapountzi

Braiek

Châteaux

et al. 2017

Sensors

122

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Electrospinning has emerged as a very powerful method combining efficiency, versatility and low cost to elaborate scalable ordered and complex nanofibrous assemblies from a rich variety of polymers. Electrospun nanofibers have demonstrated high potential for a wide spectrum of applications, including drug delivery, tissue engineering, energy conversion and storage, or physical and chemical sensors. The number of works related to biosensing devices integrating electrospun nanofibers has also increased substantially over the last decade. This review provides an overview of the current research activities and new trends in the field. Retaining the bioreceptor functionality is one of the main challenges associated with the production of nanofiber-based biosensing interfaces. The bioreceptors can be immobilized using various strategies, depending on the physical and chemical characteristics of both bioreceptors and nanofiber scaffolds, and on their interfacial interactions. The production of nanobiocomposites constituted by carbon, metal oxide or polymer electrospun nanofibers integrating bioreceptors and conductive nanomaterials (e.g., carbon nanotubes, metal nanoparticles) has been one of the major trends in the last few years. The use of electrospun nanofibers in ELISA-type bioassays, lab-on-a-chip and paper-based point-of-care devices is also highly promising. After a short and general description of electrospinning process, the different strategies to produce electrospun nanofiber biosensing interfaces are discussed.

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“…In an interesting study, [233] fabricated nanofibre-based protein microarrays through a combination of electrospinning and hydrogel lithography. Clearly defined hydrogel microstructures with incorporated nanofibres were created through electrospinning of polystyrene (PS)/poly(styrene-alt-maleic anhydride) (PSMA) fibres with diameters ranging from 0.5 to 1.0 μm followed by photopatterning of poly(ethylene glycol) (PEG) hydrogel on the electrospun fibres.…”

Section: Immunosensors Through Electrospinningmentioning

confidence: 99%

Electrospinning for High Performance Sensors

Macagnano¹,

Zampetti²,

Kny³

2015

NanoScience and Technology

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The series NanoScience and Technology is focused on the fascinating nano-world, mesoscopic physics, analysis with atomic resolution, nano and quantum-effect devices, nanomechanics and atomic-scale processes. All the basic aspects and technology-oriented developments in this emerging discipline are covered by comprehensive and timely books. The series constitutes a survey of the relevant special topics, which are presented by leading experts in the field. These books will appeal to researchers, engineers, and advanced students.More information about this series at

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Fabrication of hydrogel-micropatterned nanofibers for highly sensitive microarray-based immunosensors having additional enzyme-based sensing capability

Cited by 46 publications

References 39 publications

Electrospinning-Based Nanobiosensors

Electrospinning-Based Nanobiosensors

Recent Advances in Electrospun Nanofiber Interfaces for Biosensing Devices

Electrospinning for High Performance Sensors

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