Micropatterned biodegradable polyesters clicked with CQAASIKVAV promote cell alignment, directional migration, and neurite outgrowth

Zhang, Deteng; Wu, Shuqun; Feng, Jianyong; Duan, Yuanyuan; Xing, Dongming; Gao, Changyou

doi:10.1016/j.actbio.2018.05.018

Cited by 40 publications

(37 citation statements)

References 75 publications

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“…Achieving control over stem cell differentiation and enhance the differentiation process by non-biochemical means remains an important topic for investigation as it will lead to more advanced tissue engineering and regenerative medicine approaches. 1,2 It is well known, that many cell properties, including self-renewal and differentiation of stem cells, are regulated by (bio)chemical cues (e.g., surface chemistry, 3 extracellular matrix (ECM) proteins, 4,5 and peptides 6,7 ) and physical stimuli (surface stiffness and topography). [8][9][10][11] Cells sense such surface patterns ranging from 10 nm to 100 μm and different kinds of nanoscale and submicron topography (for example, roughness, 10 grating, 12 wrinkle, 13 pillar, 14 spiky, 15 and par-ticle 16 ) provides control over fundamental cell behaviours, for example, proliferation, migration and differentiation.…”

Section: Introductionmentioning

confidence: 99%

Topography induced stiffness alteration of stem cells influences osteogenic differentiation

Yang

Gao

et al. 2020

Biomater. Sci.

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Section: Introductionmentioning

confidence: 99%

Topography induced stiffness alteration of stem cells influences osteogenic differentiation

Yang

Gao

et al. 2020

Biomater. Sci.

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“…However, so far, the study of cell migration has mainly focused on planar substrates which are convenient for the direct observation by live microscopy and measurement of cell-substrate interactions such as cell adhesion force. The cell mobility in vitro is influenced by the gradient distribution of ligands or signaling molecules [7,8], surface topology [9], and material modulus [10]. For example, the migration rate of smooth muscle cells is mediated by the gradient distribution of VAPG on a surface [7].…”

Section: Introductionmentioning

confidence: 99%

“…(a) Fluorescent images of (1, 4, 7) EC,(2,5,8) SMC, and(3,6,9) EC-SMC spheroids after being stained by (1-3) FDA and (4-6) PI, respectively. (7-9) Corresponding merged images.…”

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confidence: 99%

Spheroids of Endothelial Cells and Vascular Smooth Muscle Cells Promote Cell Migration in Hyaluronic Acid and Fibrinogen Composite Hydrogels

et al. 2020

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Cell migration plays a pivotal role in many pathological and physiological processes. So far, most of the studies have been focused on 2-dimensional cell adhesion and migration. Herein, the migration behaviors of cell spheroids in 3D hydrogels obtained by polymerization of methacrylated hyaluronic acid (HA-MA) and fibrinogen (Fg) with different ratios were studied. The Fg could be released to the medium gradually along with time prolongation, achieving the dynamic change of hydrogel structures and properties. Three types of cell spheroids, i.e., endothelial cell (EC), smooth muscle cell (SMC), and EC-SMC spheroids, were prepared with 10,000 cells in each, whose diameters were about 343, 108, and 224 μm, respectively. The composite hydrogels with an intermediate ratio of Fg allowed the fastest 3D migration of cell spheroids. The ECs-SMCs migrated longest up to 3200 μm at day 14, whereas the SMC spheroids migrated slowest with a distance of only ~400 μm at the same period of time. The addition of free RGD or anti-CD44 could significantly reduce the migration distance, revealing that the cell-substrate interactions take the major roles and the migration is mesenchymal dependent. Moreover, addition of anti-N-cadherin and MMP inhibitors also slowed down the migration rate, demonstrating that the degradation of hydrogels and cell-cell interactions are also largely involved in the cell migration. RT-PCR measurement showed that expression of genes related to cell adhesion and antiapoptosis, and angiogenesis was all upregulated in the EC-SMC spheroids than single EC or SMC spheroids, suggesting that the use of composite cell spheroids is more promising to promote cell-substrate interactions and maintenance of cell functions.

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“…At least 100 SCs per sample were analyzed for morphology index, and the elongation ratio of SCs was calculated by the ratio of length to width. [ 25 ] The CCK‐8 assay kit (MedChemExpress. Inc., USA) was employed to evaluate the viability of SCs and N2a cells after being cultured on different films for 1 and 3 days, respectively.…”

Section: Methodsmentioning

confidence: 99%

Methylcobalamin‐Loaded PLCL Conduits Facilitate the Peripheral Nerve Regeneration

Zhang

Yang

Wang

et al. 2020

Macromolecular Bioscience

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The feasible fabrication of nerve guidance conduits (NGCs) with good biological performance is important for translation in clinics. In this study, poly(d,l‐lactide‐co‐caprolactone) (PLCL) films loaded with various amounts (wt; 5%, 15%, 25%) of methylcobalamin (MeCbl) are prepared, and are further rolled and sutured to obtain MeCbl‐loaded NGCs. The MeCbl can be released in a sustainable manner up to 21 days. The proliferation and elongation of Schwann cells, and the proliferation of Neuro2a cells are enhanced on these MeCbl‐loaded films. The MeCbl‐loaded NGCs are implanted into rats to induce the regeneration of 10 mm amputated sciatic nerve defects, showing the ability to facilitate the recovery of motor and sensory function, and to promote myelination in peripheral nerve regeneration. In particular, the 15% MeCbl‐loaded PLCL conduit exhibits the most satisfactory recovery of sciatic nerves in rats with the largest diameter and thickest myelinated fibers.

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Micropatterned biodegradable polyesters clicked with CQAASIKVAV promote cell alignment, directional migration, and neurite outgrowth

Cited by 40 publications

References 75 publications

Topography induced stiffness alteration of stem cells influences osteogenic differentiation

Topography induced stiffness alteration of stem cells influences osteogenic differentiation

Spheroids of Endothelial Cells and Vascular Smooth Muscle Cells Promote Cell Migration in Hyaluronic Acid and Fibrinogen Composite Hydrogels

Methylcobalamin‐Loaded PLCL Conduits Facilitate the Peripheral Nerve Regeneration

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