Topography induced stiffness alteration of stem cells influences osteogenic differentiation

Yang, Liangliang; Gao, Qi; Ge, Lu; Zhou, Qihui; Warszawik, Eliza M.; Bron, Reinier; Lai, King Wai Chiu; Rijn, Patrick van

doi:10.1039/d0bm00264j

Cited by 51 publications

(56 citation statements)

References 71 publications

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“…The topographies on the collagen stains are at nano-level and forced tenocytes to form an elongated shape. This result is also supported by Yang et al where they show that nano topographies as small as 50 nm led elongation and orientation of human dermal fibroblasts 37 , and human bone marrow-derived mesenchymal stem cells 38 . However, characteristics (pitch, ridge, and groove dimensions) of the nano-groove surface topography matter and can either result in elongated cell shape or lead to the formation of spherical aggregates of human tenocytes 39 .…”

Section: Discussionsupporting

confidence: 71%

Self-agglomerated collagen patterns govern cell behaviour

Eren

Wilting

et al. 2021

Sci Rep

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Reciprocity between cells and their surrounding extracellular matrix is one of the main drivers for cellular function and, in turn, matrix maintenance and remodelling. Unravelling how cells respond to their environment is key in understanding mechanisms of health and disease. In all these examples, matrix anisotropy is an important element, since it can alter the cell shape and fate. In this work, the objective is to develop and exploit easy-to-produce platforms that can be used to study the cellular response to natural proteins assembled into diverse topographical cues. We demonstrate a robust and simple approach to form collagen substrates with different topographies by evaporating droplets of a collagen solution. Upon evaporation of the collagen solution, a stain of collagen is left behind, composed of three regions with a distinct pattern: an isotropic region, a concentric ring pattern, and a radially oriented region. The formation and size of these regions can be controlled by the evaporation rate of the droplet and initial collagen concentration. The patterns form topographical cues inducing a pattern-specific cell (tenocyte) morphology, density, and proliferation. Rapid and cost-effective production of different self-agglomerated collagen topographies and their interfaces enables further study of the cell shape-phenotype relationship in vitro. Substrate topography and in analogy tissue architecture remains a cue that can and will be used to steer and understand cell function in vitro, which in turn can be applied in vivo, e.g. in optimizing tissue engineering applications.

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Section: Discussionsupporting

confidence: 71%

Self-agglomerated collagen patterns govern cell behaviour

Eren

Wilting

et al. 2021

Sci Rep

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“…At the current stage, although the role of magnetic properties and anisotropicity in the induction of ADSC osteogenic differentiation should be fully elucidated before considering animal-based validation experiments, it is still suitable in vitro platform for mechanistic studies and therapeutic candidate testing. In addition, we believe that combination with cell-derived ECM will improve the cell-composite interaction and further promote the osteogenesis of stem cells [32][33][34] .…”

Section: Resultsmentioning

confidence: 99%

Dual anisotropicity comprising 3D printed structures and magnetic nanoparticle assemblies: towards the promotion of mesenchymal stem cell osteogenic differentiation

Tang

et al. 2021

NPG Asia Mater

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Leveraging physical factors in cellular microenvironments to promote adipose tissue-derived stem cell (ADSC) osteogenic differentiation has emerged as a new strategy in the development of scaffolds for bone tissue engineering. Anisotropicity is one of those factors of interest; however, the utilization of anisotropicity to promote ADSC osteogenic differentiation is still not efficient. In this study, we designed a substrate with a dual anisotropic structure fabricated via a combination of 3D printing and magnetic field-induced magnetic nanoparticle assembly techniques. These dual anisotropic structures have a scale hierarchy, and the scale of the magnetic nanoparticle assemblies matches that of a single ADSC. This is in contrast to conventional anisotropic osteogenic induction scaffolds that have anisotropic structures at only one scale and at an order of magnitude different from single ADSCs. ADSCs cultured on substrates with such structures have significantly higher osteogenic marker expression, e.g., ALP, at both the protein and mRNA levels, and more calcium nodule formation was also found, suggesting a stronger tendency toward osteogenic differentiation of ADSCs. RNA-seq data revealed that alterations in kinase signaling pathway transduction, cell adhesion, and cytoskeletal reconstruction may account for the elevated osteogenic induction capacity. These data support our hypothesis that such a structure could maximize the anisotropicity that ADSCs can sense and therefore promote ADSC osteogenic differentiation.

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“…Moreover, cell elongation and associated cytoskeletal polymerization, influenced by stiffness and ligand type and density, can dramatically influence cell differentiation 330 , 331 also shown to be dependent on the stiffness of the cells themselves. 332 The mechanotransduction mechanism can in turn drive matrix stiffness and in one example (TRPV4 and epidermal keratinocytes) drove epithelial–mesenchymal cell type transition. 333 Further, physical cues from the matrix can change the effect of growth factors and other biochemical ligands.…”

Section: Structure and Properties Of Polymers In Bioprintingmentioning

confidence: 99%

Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine

et al. 2020

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The lack of in vitro tissue and organ models capable of mimicking human physiology severely hinders the development and clinical translation of therapies and drugs with higher in vivo efficacy. Bioprinting allow us to fill this gap and generate 3D tissue analogues with complex functional and structural organization through the precise spatial positioning of multiple materials and cells. In this review, we report the latest developments in terms of bioprinting technologies for the manufacturing of cellular constructs with particular emphasis on material extrusion, jetting, and vat photopolymerization. We then describe the different base polymers employed in the formulation of bioinks for bioprinting and examine the strategies used to tailor their properties according to both processability and tissue maturation requirements. By relating function to organization in human development, we examine the potential of pluripotent stem cells in the context of bioprinting toward a new generation of tissue models for personalized medicine. We also highlight the most relevant attempts to engineer artificial models for the study of human organogenesis, disease, and drug screening. Finally, we discuss the most pressing challenges, opportunities, and future prospects in the field of bioprinting for tissue engineering (TE) and regenerative medicine (RM).

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Topography induced stiffness alteration of stem cells influences osteogenic differentiation

Abstract: Topography-driven alterations to single cell stiffness rather than alterations in cell morphology, is the underlying driver for influencing cell biological processes, particularly stem cell differentiation.

Cited by 51 publications

References 71 publications

Self-agglomerated collagen patterns govern cell behaviour

Self-agglomerated collagen patterns govern cell behaviour

Dual anisotropicity comprising 3D printed structures and magnetic nanoparticle assemblies: towards the promotion of mesenchymal stem cell osteogenic differentiation

Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine

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