Microparticles from apoptotic monocytes induce transient platelet recruitment and tissue factor expression by cultured human vascular endothelial cells via a redox-sensitive mechanism

Essayagh, Sanah; Xuereb, Jean-Marie; Terrisse, Anne-Dominique; Tellier-Cirioni, Lise; Pipy, Bernard; Sié, P.

doi:10.1160/th07-02-0082

Cited by 65 publications

(54 citation statements)

References 27 publications

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“…The results show that endothelial activation during pregnancy is different from that in other pathological conditions involving endothelial damage such as acute coronary syndrome or diabetes mellitus [13][14][15][16][17] . These pathological situations are typically characterized by higher levels of both coagulation endothelial markers and MMPs.…”

Section: Discussionmentioning

confidence: 70%

The Pathophysiology of Endothelial Function in Pregnancy and the Usefulness of Endothelial Markers

Slavík¹,

Procházková²,

Procházka³

et al. 2011

BIOMED PAP

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Aim. The aim of this study was to assess coagulation markers of endothelial damage and examine new markers of endothelial activation such as matrix metalloproteinases (MMPs) in a group of healthy pregnant women. Matrix metalloproteinase (MMP)-2, in particular, plays a major role in the degradation of the extracellular matrix confirming its essential function in both the survival (angiogenesis) and death of endothelial cells.Detection of specific coagulation factors, mainly released from the vascular endothelium such as vWF, sTM (soluble thrombomodulin) and ePCR (endothelial protein C receptor) and factors dependent on endothelial activation such as t-PA and PAI-1, could provide information on possible endothelial dysfunction and help differentiate pregnant patients with an altered thrombotic state.Methods. Healthy pregnant women underwent complete assessment for endothelial damage (as vWF, vWF activity, sTM, ePCR, EMP, MMP-2, MMP-9 and TIMP-2) using the ELISA and other methods.Results and Conclusions. The results show that endothelial activation during pregnancy is different from that in other pathological conditions involving endothelial damage and typically characterized by higher levels of both coagulation endothelial markers and MMPs. In pregnancy, changes in extracellular matrix composition and matrix metalloproteinase activity also occur and promote vascular remodeling but, only in the uterus. Predisposing risk factors for epithelial dysfunction, and vascular mediators associated with vascular remodeling must be assessed from concentrations in whole blood. The levels of MMPs are not increased in the circulation and the local situation in the uterus cannot be monitored this way.However, MMP-2 processes and modulates the functions of many other vasoactive and pro-inflammatory molecules including adrenomedullin, big endothelin-1, calcitonin gene-related peptide, CCL7/MCP-3, CXCL12/SDF-1, galectin-3, IGFBP-3, IL-1 Beta, S100A8, and S100A9. These molecules represent new potential molecular markers of endothelial damage during pregnancy.

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Section: Discussionmentioning

confidence: 70%

The Pathophysiology of Endothelial Function in Pregnancy and the Usefulness of Endothelial Markers

Slavík¹,

Procházková²,

Procházka³

et al. 2011

BIOMED PAP

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“…Peripheral blood monocytes treated with Fas-L for 6 hours shed numerous MVs [141]. These MVs were then incubated on HUVECs, which resulted in ROS formation inhibited by antioxidant enzymes (catalase and SOD), small molecule antioxidants (N-acetylcystein and vitamin C) or a host of inhibitors of free radical-producing enzymes.…”

Section: Wbc Microvesiclesmentioning

confidence: 99%

Circulating membrane-derived microvesicles in redox biology

Larson

Hillery

Hogg

2014

Free Radical Biology and Medicine

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Microparticles or microvesicles (MV) are sub-cellular membrane blebs shed from all cells in response to various stimuli. MVs carry a battery of signaling molecules, many of them related to redox-regulated processes. The role of MVs, either as a cause or result of cellular redox signaling has been increasingly recognized over the past decade. This is in part due to advances in flow cytometry and its detection of MVs. Notably, recent studies have shown circulating MVs from platelets and endothelial cells drive reactive species-dependent angiogenesis; circulating MVs in cancer alter the microenvironment and enhance invasion through horizontal transfer of mutated proteins and nucleic acids, and harbor redox-regulated matrix metalloproteinases and pro-coagulative surface molecules; and circulating MVs from RBCs and other cells modulate cell-cell interactions through scavenging or production of nitric oxide and other free radicals. While our recognition of MVs in redox-related processes is growing, especially in the vascular biology field, much remains unknown regarding the various biologic and pathologic functions of MVs. Like reactive oxygen and nitrogen species, MVs were originally believed to have a solely a pathological role in biology. And like our understanding of reactive species, it is now clear that MVs also play an important role in normal growth, development, and homeostasis. We are just beginning to understand how MVs are involved in various biological processes—developmental, homeostatic and pathological—and the role of MVs in redox signaling is an rich and exciting area of investigation.

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“…Experimental data show that active generation of monocyte MPs results in the disruption of endothelial cell integrity and increases endothelial thrombogenicity (63 ). Enhancing coagulation activity by stimulation with monocyte MPs is associated with an increased expression of the endothelial tissue factor (TF) and a reduced expression of anticoagulant TF pathway inhibitor and thrombomodulin (63,64 ).…”

Section: Procoagulant and Antiapoptotic Role Of Mps In Endothelial Cellsmentioning

confidence: 99%

Evolving Role of Microparticles in the Pathophysiology of Endothelial Dysfunction

2013

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BACKGROUND Endothelial dysfunction is an early event in the development and progression of a wide range of cardiovascular diseases. Various human studies have identified that measures of endothelial dysfunction may offer prognostic information with respect to vascular events. Microparticles (MPs) are a heterogeneous population of small membrane fragments shed from various cell types. The endothelium is one of the primary targets of circulating MPs, and MPs isolated from blood have been considered biomarkers of vascular injury and inflammation. CONTENT This review summarizes current knowledge of the potential functional role of circulating MPs in promoting endothelial dysfunction. Cells exposed to different stimuli such as shear stress, physiological agonists, proapoptotic stimulation, or damage release MPs, which contribute to endothelial dysfunction and the development of cardiovascular diseases. Numerous studies indicate that MPs may trigger endothelial dysfunction by disrupting production of nitric oxide release from vascular endothelial cells and subsequently modifying vascular tone. Circulating MPs affect both proinflammatory and proatherosclerotic processes in endothelial cells. In addition, MPs can promote coagulation and inflammation or alter angiogenesis and apoptosis in endothelial cells. SUMMARY MPs play an important role in promoting endothelial dysfunction and may prove to be true biomarkers of disease state and progression.

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Microparticles from apoptotic monocytes induce transient platelet recruitment and tissue factor expression by cultured human vascular endothelial cells via a redox-sensitive mechanism

Cited by 65 publications

References 27 publications

The Pathophysiology of Endothelial Function in Pregnancy and the Usefulness of Endothelial Markers

The Pathophysiology of Endothelial Function in Pregnancy and the Usefulness of Endothelial Markers

Circulating membrane-derived microvesicles in redox biology

Evolving Role of Microparticles in the Pathophysiology of Endothelial Dysfunction

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