2014
DOI: 10.7754/clin.lab.2013.130731
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Microorganisms in Respiratory Tract of Patients Diagnosed with Atypical Pneumonia: Results of a Research Based on the Use of Reverse Transcription Polymerase Chain Reaction (RT-PCR) DNA

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Cited by 7 publications
(8 citation statements)
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“…Nevertheless, given the high overall sensitivity of CLART® PneumoVir, we cannot rule out the possibility of these being true positives that remained undetected by the nested PCR assay employed for resolution of discrepancies. This is in contrast to previously published data (Pillet et al, 2013;Tokman et al, 2014). The use of different criteria for resolving discordant results among the studies may account for these discrepancies.…”
Section: Discussioncontrasting
confidence: 92%
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“…Nevertheless, given the high overall sensitivity of CLART® PneumoVir, we cannot rule out the possibility of these being true positives that remained undetected by the nested PCR assay employed for resolution of discrepancies. This is in contrast to previously published data (Pillet et al, 2013;Tokman et al, 2014). The use of different criteria for resolving discordant results among the studies may account for these discrepancies.…”
Section: Discussioncontrasting
confidence: 92%
“…On the contrary, the CLART® PneumoVir was poorly sensitive for detection of picornaviruses. This has also been reported in other studies (Pillet et al, 2013;Tokman et al, 2014) and may likely be due to the fact that the assay only detects enterovirus type B (Pillet et al, 2013). False-positive results were scarce, and most frequently obtained with the CLART® PneumoVir assay, especially for hBoV and some PIVs, and notably in the context of mixed infections.…”
Section: Discussionsupporting
confidence: 73%
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“…Microarray technology showed a higher sensitivity (99.5%) and similar specificity (98.5%) to real-time PCR. However, another study with the same microarray kit used in this study showed a high specificity (100%) and low sensitivity (52%) in the clinical scenario of atypical pneumonia [ 24 ]. A study in Japan using near patient automated microarray technology showed not only a higher sensitivity and specificity compared to immunochromatographic antigen detection (the gold standard used was virus isolation) but also quicker results for children infected with Influenza and RSV [ 25 ].…”
Section: Discussionmentioning
confidence: 93%
“…While microarrays have been used extensively in the fields of cancer research and clinical genetics [8–13], more recently they have drawn growing attention for their potential application to infectious diseases [14–19]. While several studies have explored the comparative sensitivity and specificity of diagnostic microarrays in identifying a broad range of pathogens [20–32], the value and implications of their introduction to clinical practice remain undetermined. This study sought to evaluate the use of a 22-pathogen TaqMan Array Card (TAC) for diagnosing bacterial, viral and fungal pathogens in deep respiratory samples from adult ICU patients, in terms of comparative diagnostic yield, turnaround time (TAT) and influence on clinical decision-making.…”
Section: Introductionmentioning
confidence: 99%