Comparison of the performance of 2 commercial multiplex PCR platforms for detection of respiratory viruses in upper and lower tract respiratory specimens

Abstract

The performance of the CLART® PneumoVir system with that of the Luminex xTAG RVP Fast v1 assay for detection of most common respiratory viruses in upper and lower tract respiratory specimens (n=183) from unique patients with influenza-like syndrome or lower tract respiratory infection. Nested PCR coupled to automated sequencing was used for resolution of discrepancies. Fully concordant results were obtained for a total of 122 specimens, whereas 56 specimens gave partially (n=21) or fully discordant (n=35) results (Kappa coefficient, 0.62). The overall specificity of the Luminex xTAG RVP Fast v1 assay was slightly higher than that of the CLART® PneumoVir assay for human bocavirus, influenza A virus/H3N2, influenza B virus, human metapneumovirus, and parainfluenza virus, whereas the sensitivity of the latter was higher for most targeted viruses except, notably, for picornaviruses. This was irrespective of either the origin of the respiratory specimen or the age group to which the patients belonged.

Community‐acquired respiratory virus lower respiratory tract disease in allogeneic stem cell transplantation recipient: Risk factors and mortality from pulmonary virus‐bacterial mixed infections

“…The CLART® PneumoVir is able to discriminate between rhinovirus and enterovirus genus, and it permits the identification of the new influenza A/H1N1v. Overall, both technics showed comparable sensitivity for the detection of CARVs …”

“…At the HCUV, samples were tested by RT‐PCR using the Luminex xTAG RVP Fast v1 assay (Luminex Molecular Diagnostics, Toronto, ON, Canada), whereas at HLF the CLART ® PneumoVir DNA array assay (Genomica, Coslada, Spain) was performed and interpreted following the manufacturer's recommendations. Technical methodologies have been previously reported in detail elsewhere . The Luminex xTAG RVP Fast v1 assay can detect adenoviruses (ADVs); human bocavirus (HBoV); human coronavirus (CoV) types 229E, HKU1, NL63, and OC43; influenza A virus (InfA) A/H1N1, InfA/H3N2, and other InfA viruses (non‐subtypificable); influenza B virus (InfB); human metapneumovirus (HMPV) A and B; human parainfluenza virus (HPiV) 1, 2, 3, and 4A‐4B; respiratory syncytial virus (RSV) A‐B; and enterovirus/rhinovirus (EvRh).…”

Epidemiologic and Clinical Characteristics of Coronavirus and Bocavirus Respiratory Infections after Allogeneic Stem Cell Transplantation: A Prospective Single-Center Study

“…All clinical samples were tested by RT-PCR using the Luminex xTAG RVP Fast v1 assay (Luminex, Austin, TX), as described in detail elsewhere [25]. Briefly, all specimens were received at the laboratory within 30 minutes of collection and were conserved at 4°C until processed (within 18 hours of receipt).…”

Development of SPR biosensor for simultaneous detection of multiplex respiratory viruses

“…As an extension of the practical use of PCR, multiplex PCR has the potential to amplify more than one target sequence by using multiple primers in a reaction mixture without compromising the utility of the experiment [15,16]. Thus, it is an essential cost-saving technique for large scale clinical application.…”