Microneedles: Current Trends and Applications

Manoj, Hima; Gupta, Pallavi; Mohan, L.; Nagai, Masao; Wankhar, Syrpailyne; Santra, Tuhin Subhra

doi:10.1201/9781003014935-7

Cited by 11 publications

(3 citation statements)

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“…With the help of another capillary, the reagents are injected into the cytosolic part of the cell. The micromanipulation technique is comprised of a cell directly in contact with the probe, micro knife, microneedle, or microcapillary under the microscope [ 54 ]. The probe is now controlled by a micromanipulator, a macroscopic actuator.…”

Section: Single-cell Mechanical Properties For Mechanotransductionmentioning

confidence: 99%

A Review of Single-Cell Adhesion Force Kinetics and Applications

Shinde

Illath

Gupta

et al. 2021

Cells

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Cells exert, sense, and respond to the different physical forces through diverse mechanisms and translating them into biochemical signals. The adhesion of cells is crucial in various developmental functions, such as to maintain tissue morphogenesis and homeostasis and activate critical signaling pathways regulating survival, migration, gene expression, and differentiation. More importantly, any mutations of adhesion receptors can lead to developmental disorders and diseases. Thus, it is essential to understand the regulation of cell adhesion during development and its contribution to various conditions with the help of quantitative methods. The techniques involved in offering different functionalities such as surface imaging to detect forces present at the cell-matrix and deliver quantitative parameters will help characterize the changes for various diseases. Here, we have briefly reviewed single-cell mechanical properties for mechanotransduction studies using standard and recently developed techniques. This is used to functionalize from the measurement of cellular deformability to the quantification of the interaction forces generated by a cell and exerted on its surroundings at single-cell with attachment and detachment events. The adhesive force measurement for single-cell microorganisms and single-molecules is emphasized as well. This focused review should be useful in laying out experiments which would bring the method to a broader range of research in the future.

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Section: Single-cell Mechanical Properties For Mechanotransductionmentioning

confidence: 99%

A Review of Single-Cell Adhesion Force Kinetics and Applications

Shinde

Illath

Gupta

et al. 2021

Cells

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“…Lastly, a wide range of drugs and biomolecules have been delivered using microneedle platforms, with many of these studies being FDA approved and commercially marketed [ 85 , 93 , 106 ]. The skin is also a highly beneficial location for vaccination because of its large surface area and presence of immune cells in abundance [ 85 , 91 , 106 , 117 ]. Apart from its ability to transdermally deliver a wide range of biomolecules, microneedle arrays have been studied for diverse applications, such as cosmetology, ocular and gastrointestinal delivery [ 117 ], glucose sensing [ 93 , 118 ] and transdermal fluid extraction [ 93 , 119 ].…”

Section: Microfluidic Mechanoporationmentioning

confidence: 99%

Microfluidic mechanoporation for cellular delivery and analysis

Chakrabarty

Gupta

Illath

et al. 2022

Materials Today Bio

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Highly efficient intracellular delivery strategies are essential for developing therapeutic, diagnostic, biological, and various biomedical applications. The recent advancement of micro/nanotechnology has focused numerous researches towards developing microfluidic device-based strategies due to the associated high throughput delivery, cost-effectiveness, robustness, and biocompatible nature. The delivery strategies can be carrier-mediated or membrane disruption-based, where membrane disruption methods find popularity due to reduced toxicity, enhanced delivery efficiency, and cell viability. Among all of the membrane disruption techniques, the mechanoporation strategies are advantageous because of no external energy source required for membrane deformation, thereby achieving high delivery efficiencies and increased cell viability into different cell types with negligible toxicity. The past two decades have consequently seen a tremendous boost in mechanoporation-based research for intracellular delivery and cellular analysis. This article provides a brief review of the most recent developments on microfluidic-based mechanoporation strategies such as microinjection, nanoneedle arrays, cell-squeezing, and hydroporation techniques with their working principle, device fabrication, cellular delivery, and analysis. Moreover, a brief discussion of the different mechanoporation strategies integrated with other delivery methods has also been provided. Finally, the advantages, limitations, and future prospects of this technique are discussed compared to other intracellular delivery techniques.

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“…They can manipulate and control fluids in the range of micro to pico-liters, thus reducing sample loss, providing susceptible analysis in the miniaturized microfluidic systems [ 14 , 15 , 17 ]. The micro/nanofluidic devices are not only designed and fabricated to fulfill the needs of various single-cell manipulation, separation, trapping isolation and lysis, but also used for electrical, mechanical, optical, biochemical characterization, as well as for therapeutic and diagnostic purposes [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. Figure 1 shows worldwide microfluidic single-cell-related article publications in the last two decades, and it indicates the high demand of microfluidic devices for single-cell analysis and applications, evidenced by the increase in the number of scientific publications in each year.…”

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confidence: 99%