Micromonospora fluminis sp. nov., isolated from mountain river sediment

Abstract

During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38T was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus Micromonospora by means of a polyphasic taxonomy study. Strain A38T was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC15:0, 10 methyl C17:0, anteiso-C17:0 and iso-C17:0. The predominant menaquinones were MK-10(H4) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to Micromonospora tulbaghiae DSM 45142T (99.5 %), Micromonospora citrea DSM 43903T (99.4 %), Micromonospora marina DSM 45555T (99.4 %), Micromonospora maritima DSM 45782T (99.3 %), Micromonospora sediminicola DSM 45794T (99.3 %), Micromonospora aurantiaca DSM 43813T (99.2 %) and Micromonospora chaiyaphumensis DSM 45246T (99.2 %). The results of OrthoANIu analysis showed the highest similarity to Micromonospora chalcea DSM 43026T (96.4 %). However, the 16S rRNA and gyrB gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38T as a novel species. On the basis of the results presented here, we propose to classify strain A38T (=LMG 30467T=CECT 30034T) as the type strain of the novel species Micromonospora fluminis sp. nov.

Unlocking the Potential of Micromonospora sp.: Metabolomics and Molecular Modeling to Combat Antibiotic Resistance in Enteric Pathogens via ATP Synthase Inhibitors

“…The genetic identi cation of the most potent strains was a critical step toward discovering new bioactive compounds. The 16S rRNA gene analysis showed 99.6% similarity to the recently described species Micromonospora uminis, with the type strain isolated from a mountain river sediment [32] .…”

Micromonospora humida sp. nov., exhibiting antimicrobial potential, isolated from riverside soil

“…org) and potato-dextrose agar (PDA; BD). The range of growth temperatures (4,10,15,20,25,30,37, 40, 45 °C) was determined on ISP 2 for 7 days, pH (4,5,6,7,8,9,10,11) on ISP 2 broth for 7 day at 30 °C, and NaCl tolerance [0-7 % (w/v), at intervals of 1 %] on ISP 2 for 7 days at 30 °C, respectively. For pH tests, sodium acetate, potassium phosphate or sodium bicarbonate buffers were used as described previously [23].…”

“…Micromonospora is also considered as an integral part of freshwater microflora in freshwater habitats, as the presence of Micromonospora in streams, lakes, rivers and river sediments has been confirmed where they may take part in the turnover of cellulose, chitin and lignin [4]. Some recent descriptions of novel species are Micromonospora craterilacus from coastal sediment of a lake [5] and Micromonospora fluminis from river sediment [6].…”

Micromonospora cathayae sp. nov., isolated from the rhizosphere soil of Cathaya argyrophylla

“…At the time of writing, the genus Micromonospora includes over 120 species with validly published and correct names according to the List of Prokaryotic names with Standing in Nomenclature (www.bacterio.net/micromonospora.html). Members of the genus Micromonospora were found in various habitats such as muddy soil [2], rhizosphere soil [3], desert soil [4], volcanic sediment [5], mountain river sediment [6], mangrove sediment [7], marine sediment [8], plants [9–11] and insects [12]. As one of the largest genera in Actinomycetota , Micromonospora is known as an immense source of secondary metabolites [13–16].…”

“…net/micromonospora.html). Members of the genus Micromonospora were found in various habitats such as muddy soil [2], rhizosphere soil [3], desert soil [4], volcanic sediment [5], mountain river sediment [6], mangrove sediment [7], marine sediment [8], plants [9][10][11] and insects [12]. As one of the largest genera in Actinomycetota, Micromonospora is known as an immense source of secondary metabolites [13][14][15][16].…”