Micromonospora humida sp. nov., exhibiting antimicrobial potential, isolated from riverside soil

Abstract

An actinobacterial strain designated MMS20-R1-14T was isolated from a riverside soil sample. Colonies on agar plates were orange to strong orange brown in colour, which later became black. The cells grew at 10–40 °C (optimum, 37 °C), pH 5.0–11.0 (pH 8.0) and in the presence of 0–4 % NaCl (1 %). The 16S rRNA gene sequence of strain MMS20-R1-14T showed highest similarities to Micromonospora wenchangensis CCTCC AA 2012002T (99.51 %) and Micromonospora rifamycinica AM105T (99.37 %). The orthoANI values between strain MMS20-R1-14T and the two type strains were 95.72 and 90.99 %, and the digital DNA–DNA hybridization values were 63.6 and 40.8 %, respectively, thus confirming the distinction of strain MMS20-R1-14T from its mostly related species. The DNA G+C content of strain MMS20-R1-14T was 72.9 mol%. The strain contained meso-diaminopimelic acid as the major cell-wall amino acid, and the characteristic whole-cell sugars were arabinose, xylose, glucose, ribose and rhamnose. The main cellular fatty acids were C18 : 1 ω9c, iso-C15 : 0 and iso-C16 : 0, the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the predominant menaquinones were MK-10(H4) and MK-10(H6), all of which were consistent with those of Micromonospora . Strain MMS20-R1-14T showed antimicrobial activity against a range of bacterial and yeast species. The genome of the strain was found to contain 33 potential biosynthetic gene clusters for secondary metabolites, thus showing a high potential as a producer of bioactive compounds. On the basis of these phenotypic, genotypic and chemotaxonomic data, strain MMS20-R1-14T merits recognition as representing a novel species of the genus Micromonospora , for which the name Micromonospora humida sp. nov. (type strain=MMS20 R1-14T=KCTC 49541T=JCM 34494T) is proposed.

Micromonospora antibiotica sp. nov. and Micromonospora humidisoli sp. nov., two new actinobacterial species exhibiting antimicrobial potential

“…All strains were negative in tests for nitrate reduction, indole production, glucose fermentation, α-chymotrypsin, N-acetyl-β-glucosaminidase, α-mannosidase and α-fucosidase, assimilation of mannitol, N-acetyl glucosamine, capric acid, adipic acid, malic acid, citrate and phenylacetate, and hydrolysis of hypoxanthine and tyrosine. Data for growth ranges and polar lipids of compared species were taken from original studies [34][35][36][37][38], and DNA G+C contents were from the GenBank Microbial Genomes database (www.ncbi.nlm.nih.gov/genome/microbes/). All other data were obtained from this study.…”

Nocardioides okcheonensis sp. nov., isolated from riverside soil

“…Riverside soil environment harbours high bacterial diversity, from which a number of novel bacterial taxa have been reported recently [5–7]. In this study, a novel actinobacterial strain capable of degrading a wide range of substrates was isolated from riverside soil, and a new species of Nocardioides is proposed based on the polyphasic taxonomic characterization.…”

Brevibacillus humidisoli sp. nov., a moderately thermoalkaliphilic and halotolerant species isolated from riverside soil

“…Generally, phenotypic comparison of Brevibacillus is not straightforward, as most strains show poor activities in biochemical and physiological tests [6,7]. Through a series of recent studies, riverside soil has been found to be a unique environment and also a rich source of microbial diversity [8][9][10]. In this study, a novel strain designated MMS20-4M-10-Y T was isolated from riverside soil.…”

“…Through a series of recent studies, riverside soil has been found to be a unique environment and also a rich source of microbial diversity [8–10]. In this study, a novel strain designated MMS20-4M-10-Y T was isolated from riverside soil.…”