Micromixers

Nikitopoulos, Dimitris E.; Maha, Amit

doi:10.1201/9781420018677.ch7

Cited by 2 publications

(1 citation statement)

References 64 publications

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“…The first passive diffusional micromixer was designed based on previous studies [45; 46] to combine the PCR products with the ExoSAP-IT ® enzyme mixture at a 1:1 volume flow rate ratio. The diffusion channel was 34.4 mm long with a width and depth of 40 μm and 60 μm, respectively.…”

Section: Methodsmentioning

confidence: 99%

A vertically stacked, polymer, microfluidic point mutation analyzer: Rapid high accuracy detection of low-abundance K-ras mutations

Han

Lee

Nikitopoulos

et al. 2011

Analytical Biochemistry

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Recognition of point mutations in the K-ras gene can be used for the clinical management of several types of cancers. Unfortunately, several assay and hardware concerns must be addressed to allow users not well-trained in performing molecular analyses the opportunity to undertake these measurements. To provide for a larger user-base for these types of molecular assays, a vertically-stacked microfluidic analyzer with a modular architecture and process automation was developed. The analyzer employed a primary PCR coupled to an allele-specific ligase detection reaction (LDR). Each functional device, including continuous flow thermal reactors for the PCR and LDR, passive micromixers and ExoSAP-IT® purification, was designed and tested. Individual devices were fabricated in polycarbonate using hot embossing and assembled using adhesive bonding for system assembly. The system produced LDR products from a DNA sample in ~1 h, an 80% reduction in time compared to conventional bench-top instrumentation. Purifying the post-PCR products with the ExoSAP-IT® enzyme led to optimized LDR performance minimizing false positive signals and producing reliable results. Mutant alleles in genomic DNA were quantified to the level of 0.25 ng of mutant DNA in 50 ng of wild-type DNA for a 25 μL sample, equivalent to DNA from 42 mutant cells.

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Section: Methodsmentioning

confidence: 99%

A vertically stacked, polymer, microfluidic point mutation analyzer: Rapid high accuracy detection of low-abundance K-ras mutations

Han

Lee

Nikitopoulos

et al. 2011

Analytical Biochemistry

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A new class of magnetically actuated pumps and valves for microfluidic applications

Hamilton

Bryan

Gilbert

et al. 2018

Sci Rep

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We propose a new class of magnetically actuated pumps and valves that could be incorporated into microfluidic chips with no further external connections. The idea is to repurpose ferromagnetic low Reynolds number swimmers as devices capable of generating fluid flow, by restricting the swimmers’ translational degrees of freedom. We experimentally investigate the flow structure generated by a pinned swimmer in different scenarios, such as unrestricted flow around it as well as flow generated in straight, cross-shaped, Y-shaped and circular channels. This demonstrates the feasibility of incorporating the device into a channel and its capability of acting as a pump, valve and flow splitter. Different regimes could be selected by tuning the frequency and amplitude of the external magnetic field driving the swimmer, or by changing the channel orientation with respect to the field. This versatility endows the device with varied functionality which, together with the robust remote control and reproducibility, makes it a promising candidate for several applications.

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Micromixers

Cited by 2 publications

References 64 publications

A vertically stacked, polymer, microfluidic point mutation analyzer: Rapid high accuracy detection of low-abundance K-ras mutations

A vertically stacked, polymer, microfluidic point mutation analyzer: Rapid high accuracy detection of low-abundance K-ras mutations

A new class of magnetically actuated pumps and valves for microfluidic applications

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