Micromechanical response of articular cartilage to tensile load measured using nonlinear microscopy

Bell, James Stephen; Christmas, Jacqueline; Mansfield, Jessica C.; Everson, Richard; Winlove, C. Peter

doi:10.1016/j.actbio.2014.02.008

Cited by 16 publications

(20 citation statements)

References 46 publications

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“…In most vessels the volume of the adventitia fell as transmural pressure increased, suggesting that as the constraint imposed by the adventitia becomes significant (52) the inner-lying matrix is compressed, as observed in tensile testing (4). These volume changes in the extracellular matrix arise from the exudation of interstitial fluid over the timescale of minutes.…”

Section: Discussionmentioning

confidence: 95%

Microstructure and mechanics of human resistance arteries

Bell

Adio

Pitt

et al. 2016

American Journal of Physiology-Heart and Circulatory Physiology

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Section: Discussionmentioning

confidence: 95%

Microstructure and mechanics of human resistance arteries

Bell

Adio

Pitt

et al. 2016

American Journal of Physiology-Heart and Circulatory Physiology

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“…Bell et al . 65 devised new techniques based on nonlinear microscopy combined with tensile loading to detect the strain distribution in strips of adult equine metacarpophalangeal cartilage at the scale of individual cells. They found that anisotropies in the collagen network at the cellular scale gave rise to significant variations in the distribution of strain.…”

Section: Mechanotransductionmentioning

confidence: 99%

Osteoarthritis year in review 2015: mechanics

Varady

Grodzinsky

2016

Osteoarthritis and Cartilage

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Motivated by the conceptual framework of multi-scale biomechanics, this narrative review highlights recent major advances with a focus on gait and joint kinematics, then tissue-level mechanics, cell mechanics and mechanotransduction, matrix mechanics, and finally the nanoscale mechanics of matrix macromolecules. A literature review was conducted from January 2014 to April 2015 using PubMed to identify major developments in mechanics related to osteoarthritis (OA). Studies of knee adduction, flexion, rotation, and contact mechanics have extended our understanding of medial compartment loading. In turn, advances in measurement methodologies have shown how injuries to both the meniscus and ligaments, together, can alter joint kinematics. At the tissue scale, novel findings have emerged regarding the mechanics of the meniscus as well as cartilage superficial zone. Moving to the cell level, poroelastic and poroviscoelastic mechanisms underlying chondrocyte deformation have been reported, along with the response to osmotic stress. Further developments have emerged on the role of calcium signaling in chondrocyte mechanobiology, including exciting findings on the function of mechanically activated cation channels newly found to be expressed in chondrocytes. Finally, AFM-based nano-rheology systems have enabled studies of thin murine tissues and brush layers of matrix molecules over a wide range of loading rates including high rates corresponding to impact injury. With OA acknowledged to be a disease of the joint as an organ, understanding mechanical behavior at each length scale helps to elucidate the connections between cell biology, matrix biochemistry and tissue structure/function that may play a role in the pathomechanics of OA.

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“…In order to investigate potential mechanical roles of the elastin fibres in articular cartilage, we have used multiphoton microscopy to image the displacement and change in morphology of cells, collagen and elastin networks in viable, unstained cartilage subjected to applied tensile loads [15]. The reorientation of individual elastin fibres could be 4 mm Figure 2.…”

Section: Tissue Micromechanics: Mechanical Functions Of Elastic Fibresmentioning

confidence: 99%

The structure and micromechanics of elastic tissue

et al. 2014

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Elastin is a major component of tissues such as lung and blood vessels, and endows them with the long-range elasticity necessary for their physiological functions. Recent research has revealed the complexity of these elastin structures and drawn attention to the existence of extensive networks of fine elastin fibres in tissues such as articular cartilage and the intervertebral disc. Nonlinear microscopy, allowing the visualization of these structures in living tissues, is informing analysis of their mechanical properties. Elastic fibres are complex in composition and structure containing, in addition to elastin, an array of microfibrillar proteins, principally fibrillin. Raman microspectrometry and X-ray scattering have provided new insights into the mechanisms of elasticity of the individual component proteins at the molecular and fibrillar levels, but more remains to be done in understanding their mechanical interactions in composite matrices. Elastic tissue is one of the most stable components of the extracellular matrix, but impaired mechanical function is associated with ageing and diseases such as atherosclerosis and diabetes. Efforts to understand these associations through studying the effects of processes such as calcium and lipid binding and glycation on the mechanical properties of elastin preparations in vitro have produced a confusing picture, and further efforts are required to determine the molecular basis of such effects.

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Micromechanical response of articular cartilage to tensile load measured using nonlinear microscopy

Cited by 16 publications

References 46 publications

Microstructure and mechanics of human resistance arteries

Microstructure and mechanics of human resistance arteries

Osteoarthritis year in review 2015: mechanics

The structure and micromechanics of elastic tissue

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