Microlipoinjection for the Elevation of Depressed Full‐Thickness Skin Grafts on the Nose

Hambley, Richard M.; Carruthers, Jean

doi:10.1111/j.1524-4725.1992.tb02768.x

Cited by 20 publications

(5 citation statements)

References 21 publications

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“…It is best to wait at least 6 months after grafting, however, to allow time for natural correction of the scar 49. Microlipoinjection from the abdomen has been shown to be effective in elevating depressed FTSGs of the nose in some cases, but the numbers of patients have been limited 50. Poor outcomes may require surgical revision, which may include placement of another graft.…”

Section: Full‐thickness Skin Graftsmentioning

confidence: 99%

Grafts in Dermatologic Surgery

Adams¹,

Ramsey²

2005

Dermatologic Surgery

157

119

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Section: Full‐thickness Skin Graftsmentioning

confidence: 99%

Grafts in Dermatologic Surgery

Adams¹,

Ramsey²

2005

Dermatologic Surgery

157

119

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show abstract

“…[1][2][3][4] The reasons for this variability include different donor site preparation techniques, harvesting methods, instruments used, fat grafting and injection techniques, grafting interval, and methods of analysis. All of these variations have made it difficult to compare studies.…”

mentioning

confidence: 99%

Viability of Fat Cells Over Time After Syringe Suction Lipectomy

Son¹,

Oh²,

Choi³

et al. 2010

Annals of Plastic Surgery

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The purpose of this study was to determine the late decline in viability of fat cells over time for fat tissue stored at -15 degrees C and -70 degrees C after harvest from abdominal liposuction. A total of 16 females were recruited for this study. The viability of fat cell specimens was measured after freezing for 1, 3, 7, 14, 28, and 56 days. A number of viable mature adipocytes were evaluated by fluorescence microscopy after staining with fluorescein diacetate and propidium iodide. The glycerol-3-phosphate dehydrogenase activity was measured in lipoaspirates before digestion and the XTT reduction assay was performed. In addition, the XTT reduction assay was also performed on isolated lipocytes and preadipocytes.The viability of mature adipocytes was very low for both the -15 degrees C and -70 degrees C samples after 1 day of freezing (13.3% +/- 7.4% and 12.6% +/- 6.3%, respectively). There was no statistically significant difference between the samples stored at the 2 temperatures. The GPDH activity of the lipoaspirates frozen, for 1 day, at -15 degrees C and -70 degrees C was 25.1% +/- 10% and 28.7% +/- 11%, respectively. For the XTT test, the fractional enzyme activity of the lipoaspirates frozen, for 1 day, at -15 degrees C and -70 degrees C was 30.0% +/- 10.9% and 36.1% +/- 12.3%, respectively. In addition, the adipocytes had low activity from day one: 15.4% +/- 7.2% at -15 degrees C and 11.5% +/- 5.6% at -70 degrees C. Furthermore, the preadipocytes had a low activity of 8.0% +/- 6.0% at -15 degrees C and 8.6% +/- 3.8% at -70 degrees C. At 8 weeks, there were few viable mature adipocytes and the activity of the cells was very low by XTT and GPDH testing.The results of this study showed that the viability of adipocytes declined rapidly after frozen storage for 1 day at both -15 degrees C and -70 degrees C, and decreased gradually in storage after 8 weeks; at which time only approximately 5% of the fat cells were alive. These findings suggest that the present fat preservation storage techniques using a -15 degrees C freezer or a -70 degrees C deep freezer are both inadequate to maintain the viability of fat cells.

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“…They recommended to use minimally invasive subdermal dissection technique and where possible to correct large depressions repeating two or three times the grafting procedures, to prevent fat resorption and skin necrosis [21]. …”

Section: Discussionmentioning

confidence: 99%

Fat and epidermal cell suspension grafting: a new advanced one-step skin regeneration surgical technique

Migliano

Bellei

Govoni

et al. 2014

J Exp Clin Cancer Res

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BackgroundDystrophic skin scarring commonly occurs following skin cancer resections. In particular, the cosmetic outcome of skin graft reconstructions, following epidermoidal carcinoma removal, is generally poor due to wide marginal tumour excision, loss of subcutaneous tissues, and subsequent pigmented atrophic scarring of the graft coverage. Skin grafting sequelae need a three dimensional correction to restore either the epidermal layer or the dermal/subdermal volume and vascularization.MethodsThe surgeons combined CO2 laser ablation, subdermal lipofilling according to the Coleman’s technique and epidermal cell suspension autografting to correct wide depressed and dyschromic facial scar. The Authors applied this new technique on three nasal skin cancer resected patients: two of them actually need a longer follow-up, the third patient, a 48 yr old caucasian male, presented a skin grafting scar due to sclerodermiform basal cell carcinoma removal. This case is reported discussing pre-intra and post-operative records up to a complete twelve months follow-up.ResultsRecords at six and twelve months follow-up after surgery demonstrate a fully integrated skin graft and a good restoration of the treated area, presenting the same texture and pigmentation of the adjacent untreated skin. Optimal, stable three-dimensional skin cosmetic restoration was obtained in a single stage surgical procedure.ConclusionAutologous non-cultured epidermal cell suspension transplantation on an epidermal laser ablated skin area, in combination with lipofilling subdermal reconstruction, appears to be an effective, simple and time-saving method to correct skin graft sequelae, in skin cancer patients. This new technique allows to restore a three-dimensional morphological structure of the treated area and to recover a natural appearance of the skin at the same time. The Authors believe that this technique can be safely used to treat any kind of dystrophic scarring.

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Microlipoinjection for the Elevation of Depressed Full‐Thickness Skin Grafts on the Nose

Abstract: Microlipoinjection can provide cosmetically useful soft tissue augmentation under depressed full-thickness skin grafts; this augmentation can persist.

Cited by 20 publications

References 21 publications

Grafts in Dermatologic Surgery

Grafts in Dermatologic Surgery

Viability of Fat Cells Over Time After Syringe Suction Lipectomy

Fat and epidermal cell suspension grafting: a new advanced one-step skin regeneration surgical technique

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