Microlens characterization by digital holographic microscopy with physical spherical phase compensation

Abstract

Microlenses have been characterized by a digital holographic microscopy system, which is immune to the inherent wavefront aberration. The digital holographic microscopy system takes advantage of fiber optics and uses the light emitted directly from a single-mode fiber as the recording reference wave. By using such a reference beam, which is quasi-identical to the object beam, the inherent wavefront aberration of the digital holographic microscope is removed. The alignment of the optical setup can be optimized with the help of numerical reconstruction software to give the system phase with the off-axis tilt removed. There is one, and only one, reference fiber point position to give a reference wavefront that is quasi-identical to the object wavefront where the system is free of wavefront aberration and directly gives the quantitative phase of the test object without the need for complicated numerical compensation.

Coded aperture correlation holographic microscope for single-shot quantitative phase and amplitude imaging with extended field of view

“…This powerful tool enables three-dimensional (3D) non-destructive imaging, in a nanoscale sensitivity, and enables reconstructing the morphology of phase objects that are otherwise invisible to the naked eye. Quantitative phase imaging finds a wide range of applications including stain-free biological cell imaging [2], non-destructive quality tests of optical elements [3] and surface profilometry [4].…”

Investigation of osteoblast cells behavior in polymeric 3D micropatterned scaffolds using digital holographic microscopy

“…Digital holographic microscopy (DHM) technique is a suitable method to study these samples in a noninvasive manner while providing quantitative information. The advantages of DHM for micrometric investigations have already been demonstrated; DHM is a noncontact, markerfree technique suitable for transparent samples (in the visible range), such as microstructures [17] or biological specimens in their natural medium [18][19][20]. The experimental procedure for image acquisition does not require mechanical scanning; it is a single-shot imaging technique used for fast processes visualization, completed also by the advantage of providing nanometric resolution about the sample heights [21][22][23][24][25].…”

Deep walls microscaffold characterization using digital holographic microscopy

“…Digital holographic microscopy (DHM) is a noncontact, marker free technique suitable for transparent sample investigations in visible range, such as biological specimens or microstructures [1][2][3]. It is a single-shot imaging technique used for fast processes visualization with nanometric resolution along the propagation axis [4][5][6][7].…”