Deep walls microscaffold characterization using digital holographic microscopy

Mihăilescu, Mona; Păun, Irina Alexandra; Popescu, Roxana-Cristina; Matei, A.; Acasandrei, A.; Dinescu, M.; Scarlat, Eugen I.

doi:10.1109/cleopr.2013.6600590

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…where λ 632.8 nm is the wavelength of the laser, n 1 and n 2 are the refractive indices for each liquid medium, respectively [37]. The phase shift is calculated regarding a beam that travels through the cell and one that travels besides the cell (very close to its edge).…”

Section: Numerical Proceduresmentioning

confidence: 99%

Investigation of osteoblast cells behavior in polymeric 3D micropatterned scaffolds using digital holographic microscopy

Mihăilescu¹,

Popescu²,

Matei³

et al. 2014

Appl. Opt.

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The effect of micropatterned polymeric scaffolds on the features of the cultured cells at different time intervals after seeding was investigated by digital holographic microscopy. Both parallel and perpendicular walls, with different heights, were fabricated using two-photon lithography on photopolymers. The walls were subsequently coated with polypyrrole-based thin films using the matrix assisted pulsed laser evaporation technique. Osteoblast-like cells, MG-63 line, were cultured on these polymeric 3D micropatterned scaffolds. To analyze these scaffolds with/without cultured cells, an inverted digital holographic microscope, which provides 3D images, was used. Information about the samples' refractive indices and heights was obtained from the phase shift introduced in the optical path. Characteristics of cell adhesion, alignment, orientation, and morphology as a function of the wall heights and time from seeding were highlighted.

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Section: Numerical Proceduresmentioning

confidence: 99%