Microgrooved patterns enhanced PC12 cell growth, orientation, neurite elongation, and neuritogenesis

Abstract

Understanding neurite outgrowth, orientation, and migration is important for the design of biomaterials that interface with the neuronal tissue. Micropatterns can significantly influence neurite outgrowth, neurite length, orientation, extracellular matrix expression, neuron differentiation, and migrating velocity. We analyzed the neuritogenesis and neurite outgrowth of PC12 cells in three-dimensional Si wafer with various micropatterns fabricated using photolithography and etching techniques. When nerve growth factor was added into culture medium, PC12 cells started to grow neurites. Extended neurites were significantly affected by different patterns and displayed higher growth-associated protein-43 expression. Cellular performance including growth rate, bipolar phenotype elongation, neurite extension, and growth-associated protein-43 expression of the PC12 cells with a differentiated character are higher on a grooved substrate. However, the grooved pattern can restrict the motility of PC12 cells and decrease the velocity of cellular movement. The average of the number of neurites per cell is the highest on the pillar substrate, but their neurite length is the shortest. In contrast, actin and lamimin expression, motion track, angular deviation, and movement velocity of PC12 cells are most excellent on the planar Si wafer. These findings confirmed that topographical features can cooperatively act with nerve growth factor, signaling the regulation of the formation of neurites.

Osteogenic differentiation of stem cells from human exfoliated deciduous teeth on poly(ε-caprolactone) nanofibers containing strontium phosphate

“…An ideal synthetic bone scaffold should support osteoblastic phenotype behavior, particularly, bone matrix deposition. Tissue scaffolds with microscale to nanoscale features support greater cellular adhesion and phenotypic activity than planar scaffolds [22][23][24]. Textured scaffolds mimic the highly featured native-tissue architecture exhibited in numerous natural tissues.…”

Facile Synthesis of Conductive Polypyrrole Wrinkle Topographies on Polydimethylsiloxane via a Swelling–Deswelling Process and Their Potential Uses in Tissue Engineering

“…57 Su et al studied the effects of micropatterns on PC12 cell behaviors and found that the cells cultured on Si microgrooved patterns extended 2 to 3 times longer neurites on the micropatterns. 58 prefer differentiation (i.e., neurite formation) to proliferation, whereas the cells on the smooth substrate tend to induce proliferation rather than neurite formation. Regarding neurite elongation, in our study, no significant differences in neurite length were found between the wPPy and 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 19 smooth PPy substrates during the early days of culture.…”

Nanogrooved Surface-Patterns induce cellular organization and axonal outgrowth in neuron-like PC12-Cells

“…Extensive research to date has been performed on topographies in the micrometre range. Such studies reported neuronal alignment and axonal outgrowth, although the microgrooves reached supra-cellular dimensions (Tuft and et al, 2013;Clark and et al, 1990;Beduer and et al, 2012;Su and et al, 2013;Morelli and et al, 2010;Miller et al, 2002;Hirono and et al, 1988;Walsh et al, 2005). Several groups demonstrated a maximum pitch threshold for alignment and a negative correlation between alignment and increasing pitch size (Tuft and et al, 2013;Beduer and et al, 2012;Yao and et al, 2009).…”