Microgels as carriers of antimicrobial peptides – Effects of peptide PEGylation

Nordström, Randi; Nyström, Lina; Ilyas, Humaira; Atreya, Hanudatta S.; Borro, Bruno C.; Bhunia, Anirban; Malmsten, Martin

doi:10.1016/j.colsurfa.2018.12.049

Cited by 27 publications

(28 citation statements)

References 39 publications

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“…Most of the work done regarding microgels with antimicrobial activity deals with the drug delivery ability of microgels capable to carry and release antibiotics or antimicrobial agents [22,23,24,25]. Malmsten et al first studied the existing interactions between antimicrobial peptides (AMP) and poly(acrylic acid) microgels and peptide uptake and sustained release [26,27,28].…”

Section: Introductionmentioning

confidence: 99%

Thermoresponsive Poly(N-Isopropylacrylamide-co-Dimethylaminoethyl Methacrylate) Microgel Aqueous Dispersions with Potential Antimicrobial Properties

et al. 2019

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The work herein describes the preparation of thermoresponsive microgels with potential antimicrobial properties. Most of the work performed so far regarding microgels with antimicrobial activity, deals with the ability of microgels to carry and release antibiotics or antimicrobial agents (antimicrobial peptides). The originality of this work lies in the possibility of developing intrinsic antimicrobial microgels by copolymerization of the well-known thermoresponsive monomer, N-isopropylacrylamide (NIPAM) with dimethylaminoethyl methacrylate (DMAEMA), a water-soluble monomer, to form microgels via precipitation polymerization (radical polymerization). Due to the presence of a tertiary amine in the DMAEMA comonomer, microgels can be modified by N-alkylation reaction with methyl and butyl iodide. This quaternization confers positive charges to the microgel surfaces and thus the potential antimicrobial activity. The effect of DMAEMA content and its quaternization with both, methyl and butyl iodide is evaluated in terms of thermal and surface charge properties, as well as in the microgel size and viscoelastic behavior. Finally, a preliminary study of the antimicrobial activity against different microorganisms is also performed in terms of minimum inhibitory concentration (MIC). From this study we determined that in contrast with butylated microgels, methylated ones show potential antimicrobial activity and good physical properties besides of maintaining microgel thermo-responsiveness.

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Section: Introductionmentioning

confidence: 99%

Thermoresponsive Poly(N-Isopropylacrylamide-co-Dimethylaminoethyl Methacrylate) Microgel Aqueous Dispersions with Potential Antimicrobial Properties

et al. 2019

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“…[9] As a result, the majority of clinical applicable AMPs are used or designed for topical applications only with very few exceptions aimed for systematic applications. [5] To overcome these limitations, many different strategies, including but not limited to structural/ sequence modification, [10][11][12][13] specific delivery system design, [14][15][16][17] "smart" AMPs [18][19][20] and de novo designed antimicrobial peptides/peptidomimetics [21][22][23][24][25][26][27] have been developed to efficiently use AMPs without being hindered by their side-effects. Among these strategies, modification or mimicry of naturally occurring peptides with natural amino acids or unnatural building blocks is an efficient pathway.…”

Section: Introductionmentioning

confidence: 99%

Gramicidin‐S‐Inspired Cyclopeptidomimetics as Potent Membrane‐Active Bactericidal Agents with Therapeutic Potential

Wen

Huang

et al. 2020

ChemMedChem

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Antimicrobial peptides (AMPs) are promising antibacterial agents often hindered by their undesired hemolytic activity. Inspired by gramicidin S (GS), a well‐known cyclodecapeptide, we synthesized a panel of antibacterial cyclopeptidomimetics using β,γ‐diamino acids (β,γ‐DiAAs). We observed that peptidomimetic CP‐2 displays a bactericidal activity similar to that of GS while possessing lower side‐effects. Moreover, extensive studies revealed that CP‐2 likely kills bacteria through membrane disruption. Altogether, CP‐2 is a promising membrane‐active antibiotic with therapeutic potential.

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“…However, some undesirable characteristics, such as high toxicity, easy degradation, and low bioavailability by binding to serum proteins, mucins, and other anionic components, have impaired the clinical translation of AMPs [ 240 , 241 ]. Therefore, recently increased attention has been placed on nanogels as carriers for the delivery of AMPs to improve the antimicrobial performance [ [243] , [244] , [245] , [246] , [247] , [248] , [249] , [250] ]. The Malmsten group investigated cationic AMPs incorporated into anionic poly (ethyl acrylate- co -methacrylic acid) (MAA) nanogels in different ways that influenced the encapsulation efficiency and release of AMPs, and the membrane interactions and antimicrobial effects of those nanogels.…”

Section: Nanogel-based Antimicrobial Systemsmentioning

confidence: 99%

Nanogels: A novel approach in antimicrobial delivery systems and antimicrobial coatings

Keskin

Forson

et al. 2021

Bioactive Materials

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The implementation of nanotechnology to develop efficient antimicrobial systems has a significant impact on the prospects of the biomedical field. Nanogels are soft polymeric particles with an internally cross-linked structure, which behave as hydrogels and can be reversibly hydrated/dehydrated (swollen/shrunken) by the dispersing solvent and external stimuli. Their excellent properties, such as biocompatibility, colloidal stability, high water content, desirable mechanical properties, tunable chemical functionalities, and interior gel-like network for the incorporation of biomolecules, make them fascinating in the field of biological/biomedical applications. In this review, various approaches will be discussed and compared to the newly developed nanogel technology in terms of efficiency and applicability for determining their potential role in combating infections in the biomedical area including implant-associated infections.

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Microgels as carriers of antimicrobial peptides – Effects of peptide PEGylation

Cited by 27 publications

References 39 publications

Thermoresponsive Poly(N-Isopropylacrylamide-co-Dimethylaminoethyl Methacrylate) Microgel Aqueous Dispersions with Potential Antimicrobial Properties

Thermoresponsive Poly(N-Isopropylacrylamide-co-Dimethylaminoethyl Methacrylate) Microgel Aqueous Dispersions with Potential Antimicrobial Properties

Gramicidin‐S‐Inspired Cyclopeptidomimetics as Potent Membrane‐Active Bactericidal Agents with Therapeutic Potential

Nanogels: A novel approach in antimicrobial delivery systems and antimicrobial coatings

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