Microgel particles containing methacrylic acid: pH-triggered swelling behaviour and potential for biomaterial application

Lally, Sarah; MacKenzie, Paul D.; Maitre, Christine L. Le; Freemont, Anthony J.; Saunders, Brian R.

doi:10.1016/j.jcis.2007.08.030

Cited by 43 publications

(31 citation statements)

References 18 publications

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“…However, the osteogenic differentiation of hMSC demonstrated here was performed with one donor at passage nine; therefore, caution in interpreting these results should be considered. A variety of bioactive injectable hydrogel scaffolds have been reported; however, many systems preclude the incorporation of cells in the liquid state as they require co-injection with cross linking agents (Halacheva et al, 2014;Lally et al, 2007;Shin et al, 2003). Other systems are too viscous for injection through fine-bore needles, which may limit the potential number of bone repair clinical applications and result in further damage to the target site (Michalek et al, 2010;Nassr et al, 2009).…”

Section: Induction Of Osteogenic Differentiationmentioning

confidence: 99%

“…Furthermore, dispersed mineral within biomaterial scaffolds has been shown to enhance mechanical properties (Sinha et al, 2007), provide nucleation sites for further HAP deposition, as well as providing critical cellular anchoring points, which not only enable integration with surrounding bone tissue, but have also been shown to regulate the fate of cellular differentiation Rea et al, 2004;Trappmann et al, 2012;Zhao et al, 2006). A variety of injectable hydrogel scaffolds for tissue regeneration have been reported (D'Este and Eglin, 2013); however, many systems require co-injection with cross linking agents (Halacheva et al, 2014;Lally et al, 2007;Shin et al, 2003) which can result in toxicity to the delivered cells as well as the surrounding tissues during injection. Other systems are too viscous for injection through fine-bore needles, which may damage the target site and limit the potential of clinical applications (Michalek et al, 2010;Nassr et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Hydroxyapatite nanoparticle injectable hydrogel scaffold to support osteogenic differentiation of human mesenchymal stem cells

Thorpe

Creasey²,

Sammon³

et al. 2016

eCM

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Bone loss associated with degenerative disease and trauma is a clinical problem increasing with the aging population. Thus, effective bone augmentation strategies are required; however, many have the disadvantages that they require invasive surgery and often the addition of expensive growth factors to induce osteoblast differentiation. Here, we investigated a Laponite  crosslinked, pNIPAMDMAc copolymer (L-pNIPAM-co-DMAc) hydrogel with hydroxyapatite nanoparticles (HAPna), which can be maintained as a liquid ex vivo, injected via narrowgauge needle into affected bone, followed by in situ gelation to deliver and induce osteogenic differentiation of human mesenchymal stem cells (hMSC). L-pNIPAMco-DMAc hydrogels were synthesised and HAPna added post polymerisation. Commercial hMSCs from one donor (Lonza) were incorporated in liquid hydrogel, the mixture solidified and cultured for up to 6 weeks. Viability of hMSCs was maintained within hydrogel constructs containing 0.5 mg/mL HAPna. SEM analysis demonstrated matrix deposition in cellular hydrogels which were absent in acellular controls. A significant increase in storage modulus (G') was observed in cellular hydrogels with 0.5 mg/mL HAPna. Semi-quantitative immunohistochemistry and histological analysis demonstrated that bone differentiation markers and collagen deposition was induced within 48 h, with increased calcium deposition with time. The thermally triggered hydrogel system, described here, was sufficient without the need of additional growth factors or osteogenic media to induce osteogenic differentiation of commercial hMSCs. Preliminary data presented here will be expanded on multiple patient samples to ensure differentiation is seen in these samples. This system could potentially reduce treatment costs and simplify the treatment strategy for orthopaedic repair and regeneration.

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Section: Induction Of Osteogenic Differentiationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite nanoparticle injectable hydrogel scaffold to support osteogenic differentiation of human mesenchymal stem cells

Thorpe

Creasey²,

Sammon³

et al. 2016

eCM

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“…These microgels have previously been well characterized for various applications [22][23][24] and, importantly, the co-monomer composition can be easily varied during synthesis to alter microgel swelling properties, allowing for tunable performance of the system. 25 In the present study we employed this microgel platform in combination with the model cationic peptide poly-L-lysine (pLys, pI≈9 (10.5 for isolated lysine monomers 12 )).…”

Section: Introductionmentioning

confidence: 99%

Factors Affecting Peptide Interactions with Surface-Bound Microgels

et al. 2016

Self Cite

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Effects of electrostatics and peptide size on peptide interactions with surfacebound microgels were investigated with ellipsometry, confocal microscopy, and atomic force microscopy (AFM). Results show that binding of cationic poly-L-lysine (pLys) to anionic, covalently immobilized, poly(ethyl acrylate-co-methacrylic acid) microgels increased with increasing peptide net charge and microgel charge density. Furthermore, peptide release was facilitated by decreasing either microgel or peptide charge density. Analogously, increasing ionic strength facilitated peptide release for short peptides. As a result of peptide binding, the surface-bound microgels displayed pronounced deswelling and increased mechanical rigidity, the latter quantified by quantitative nanomechanical mapping. While short pLys was found to penetrate the entire microgel network and to result in almost complete charge neutralization, larger peptides were partially excluded from the microgel network, forming an outer peptide layer on the microgels. As a result of this difference, microgel flattening was more influenced by the lower Mw peptide than the higher. Peptide-induced deswelling was found to be lower for higher Mw pLys, the latter effect not observed for the corresponding microgels in the dispersed state. While the effects of electrostatics on peptide loading and release were similar to those observed for dispersed microgels, there were thus considerable effects of the underlying surface on peptide-induced microgel deswelling, which need to be considered in the design of surface-bound microgels as carriers of peptide loads, e.g., in drug delivery or in functionalized biomaterials.

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“…These materials are of great interest since they find several applications as separation membranes 5 , sensors 6 , adsorbents, medical and pharmaceutical drug delivery systems, as well as in other various nanotechnology fields [7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization and Swelling study of Poly (Methacrylic acid-co-Maleic acid) Hydrogels

Belkadi¹,

Bendaikha²,

Lebsir³

et al. 2018

Orient. J. Chem

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A series of poly (methacrylic acid-Co-maleic acid), P(MAA-MA), hydrogels have been prepared by free radical polymerization in aqueous medium using different amount of maleic acid (MA) (0-5 wt.%), initiated by potassium persulfate (KPS) in the presence of N,N'-methylenebisacrylamide (MBA) as a crosslinking agent. Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscope (SEM) techniques were used to characterize the prepared hydrogels. Their swelling behaviours were investigated according to the maleic acid content and pH of solution. Kinetic swelling of hydrogels was studied too. The results showed that the swelling ratios increase with increasing the maleic acid content. Kinetic studies showed good fitting to pseudo-second order equation. The prepared hydrogels obeyed non-Fickian water diffusion.

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Microgel particles containing methacrylic acid: pH-triggered swelling behaviour and potential for biomaterial application

Cited by 43 publications

References 18 publications

Hydroxyapatite nanoparticle injectable hydrogel scaffold to support osteogenic differentiation of human mesenchymal stem cells

Hydroxyapatite nanoparticle injectable hydrogel scaffold to support osteogenic differentiation of human mesenchymal stem cells

Factors Affecting Peptide Interactions with Surface-Bound Microgels

Synthesis, Characterization and Swelling study of Poly (Methacrylic acid-co-Maleic acid) Hydrogels

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