Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects

Betti, Cecilia; Davini, Tania; Giannessi, L.; Loprieno, N.; Barale, Roberto

doi:10.1016/0027-5107(94)90306-9

Cited by 164 publications

(64 citation statements)

References 28 publications

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“…Exposure to X-rays may cause deterministic and stochastic effects in living organisms and a lead apron can be used to protect personnel from this radiation 29) . The findings of this study indicated a significant decrease in DNA damage resulting from use of protective lead aprons by the radiology personnel.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of DNA damage in lymphocytes of radiology personnel by comet assay

Khisroon

Khan²,

Naseem³

et al. 2015

Journal of Occupational Health

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Section: Discussionmentioning

confidence: 99%

Evaluation of DNA damage in lymphocytes of radiology personnel by comet assay

Khisroon

Khan²,

Naseem³

et al. 2015

Journal of Occupational Health

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“…In previous human biomonitoring studies by using comet assay, DNA damage was evaluated either on isolated lymphocytes [7,9,32,33] or on peripheral white blood cells [11,12]. As reported by Kevekordes et al [6], the use of the fresh blood has an advantage of detecting acute effects on DNA, while consisting of two thirds of shortliving non-lymphocyte nuclear blood cells (i.e., monocytes and granulocytes).…”

Section: Discussionmentioning

confidence: 99%

“…In recent molecular epidemiology studies, DNA damage evaluated by the alkaline single-cell microgel electrophoresis (SCGE) or comet assay is frequently utilized as a "biomarker of exposure" [7][8][9][10][11][12][13]. This simple fluorescence microscopic method allows for the detection of primary DNA damage and the measuring of repair kinetics at the level of single cells [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Assessment of chemotherapy‐induced DNA damage in peripheral blood leukocytes of cancer patients using the alkaline comet assay

Kopjar

Garaj‐Vrhovac

Milas

2001

Teratog Carcinog Mutagen

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The alkaline comet assay was employed to assess the pre-and post-treatment levels of in vivo DNA damage in peripheral blood leukocytes of cancer patients. During the study all patients were given antineoplastic drugs, mainly as polychemotherapy. To quantify the DNA damage, two different comet parameters were evaluated: the tail length and the tail moment. Our results indicate marked interindividual variations between baseline DNA damage in peripheral blood leukocytes recorded among cancer patients prior to the chemotherapy. After intravenous administration of various antineoplastic drugs, a significantly increased level of DNA damage in all cancer patients compared to their pre-treatment values was recorded The highest level of DNA damage was seen following administration of 5-fluorouracil, adriamycin, and cisplatin (FAP protocol). The results indicate that administration of antineoplastic drugs in standard protocols is accompanied by significant DNA damage in peripheral blood leukocytes. In order to diminish the potential risks of developing second neoplasms, a continuous biomonitoring of cancer patients after the ending of chemotherapy becomes important. Despite their limitations, present results confirm the usefulness of the alkaline comet assay as a sensitive biomarker of exposure that enables rapid and simple detection of primary DNA damage in peripheral blood leukocytes of cancer patients. Together with standard cytogenetic endpoints, the comet assay provides a powerful technique for the routine detection of critical DNA lesions produced after administration of antineoplastic drugs in the clinical settings. Teratogenesis Carcinog. Mutagen. 22:13-30, 2002.

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“…Una de las técnicas utilizada para esta vigilancia es el ensayo del cometa o la electroforesis alcalina de células individuales, que permite evaluar el daño causado en el material genético por diferentes agentes químicos y físicos (21)(22)(23). Tiene amplias aplicaciones en toxicología genética, en ensayos de genotoxicidad in vitro (24)(25)(26)(27)(28)(29) e in vivo (30)(31)(32), en vigilancia biológica ambiental y en vigilancia de poblaciones humanas expuestas a diversas sustancias químicas mutagénicas (33)(34)(35)(36)(37)(38)(39)(40).…”

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Evaluación del daño en el ADN y vigilancia biológica de la exposición laboral a solventes orgánicos, 2006

et al. 2008

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Introducción. La exposición a solventes es uno de los mayores riesgos potenciales para millones de trabajadores en el mundo; los solventes generan contaminación ambiental y desencadenan problemas de salud pública. Objetivo. Determinar los niveles de los metabolitos benceno, tolueno y xileno, los polimorfismos de las enzimas CYP2E1, GSTM1, GSTT1 y el daño del ADN mediante el ensayo del cometa. Materiales y métodos. Se llevó a cabo un estudio descriptivo de corte transversal para la determinación de polimorfismos genéticos y prueba del cometa en 90 trabajadores pertenecientes a cinco empresas. Se aplicó una encuesta, se tomaron muestras de sangre y de orina, se midieron las concentraciones de fenol, ácido hipúrico orto y meta-metilhipúrico. Se hizo el análisis estadístico y se exploraron posibles asociaciones. Resultados. El 34,4% eran trabajadores con exposición directa a solventes. En este grupo se evidenciaron concentraciones superiores a los límites permisibles en 3,3% para fenol, en 6,6% para ácido hipúrico, en 3,3% para ácido orto-metilhipúrico y en 36,7% para ácido metametilhipúrico, mayor longitud promedio de la cola del cometa (19,5 µm) y un incremento del porcentaje de células con daño medio (19,0%) (p=0,0007). El porcentaje de individuos expuestos con genotipos ausentes para las enzimas GSTT1 y GSTM1 fue de 46,7% y de 56,8%, respectivamente. Conclusión. El uso de biomarcadores de exposición, efecto y susceptibilidad, se ha convertido en una herramienta fundamental para la evaluación del riesgo asociado con la exposición a agentes tóxicos.Palabras clave: daño del ADN/genética, exposición profesional, solventes/toxicidad, salud pública DNA damage assessment and biological monitoring of occupational exposure to organic solvents, 2006Introduction. Exposure to solvents is one of the highest potential risks for millions of workers in the world; they can generate substantial environmental pollution leading to outbreaks of public health problems. Objective. Blood levels were determined for metabolites of benzene, toluene and xylene, and polymorphisms for enzymes CYP2E1, GSTM1, GSTT1 were characterized. Damage to DNA was assessed by the comet assay for exposure to organic solvents. Materials and methods. A cross-sectional study was undertaken in 90 employees from 5 different companies. A survey form was administered; blood was sampled to detect the genetic polymorphisms and to apply the comet assay (single cell gel electrophoresis) to detect DNA fragmentation. The concentrations of phenol, ortho and meta methylhippuric acids were measured in urine. Statistical analyses explored the possible associations. Results. The percentage of workers directly exposed to solvents was 34.4%. In this group, the evidence indicated concentrations higher than the permitted limits: 3.3% for phenol, 6.6% for

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Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects

Cited by 164 publications

References 28 publications

Evaluation of DNA damage in lymphocytes of radiology personnel by comet assay

Evaluation of DNA damage in lymphocytes of radiology personnel by comet assay

Assessment of chemotherapy‐induced DNA damage in peripheral blood leukocytes of cancer patients using the alkaline comet assay

Evaluación del daño en el ADN y vigilancia biológica de la exposición laboral a solventes orgánicos, 2006

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