Microfungi from the Ectorhizosphere-Rhizoplane Zone of Different Halophytic Plants from the Red Sea Coast of Egypt

El-Morsy, El-Sayed M.

doi:10.2307/3761367

Cited by 19 publications

(13 citation statements)

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“…Comparisons between the herein recovered fungal diversity and that obtained in other works are difficult due to the relative scarcity of similar samplings. In general terms, we cannot conclude that the fungal community reported here is highly divergent from that found in other studies (e.g., [16,[42][43][44]). Most fungi identified are common endophytes and soil dwellers, albeit, in other cases, BLAST identities matched uncultured fungi, indicating a possible novelty in the strains.…”

Section: Discussioncontrasting

confidence: 99%

Fungal Assemblages Associated with Roots of Halophytic and Non-halophytic Plant Species Vary Differentially Along a Salinity Gradient

et al. 2012

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Structure of fungal communities is known to be influenced by host plants and environmental conditions. However, in most cases, the dynamics of these variation patterns are poorly understood. In this work, we compared richness, diversity, and composition between assemblages of endophytic and rhizospheric fungi associated to roots of two plants with different lifestyles: the halophyte Inula crithmoides and the non-halophyte I. viscosa (syn. Dittrichia viscosa L.), along a spatially short salinity gradient. Roots and rhizospheric soil from these plants were collected at three points between a salt marsh and a sand dune, and fungi were isolated and characterized by ITS rDNA sequencing. Isolates were classified in a total of 90 operational taxonomic units (OTUs), belonging to 17 fungal orders within Ascomycota and Basidiomycota. Species composition of endophytic and soil communities significantly differed across samples. Endophyte communities of I. crithmoides and I. viscosa were only similar in the intermediate zone between the salt marsh and the dune, and while the latter displayed a single, generalist association of endophytes, I. crithmoides harbored different assemblages along the gradient, adapted to the specific soil conditions. In the lower salt marsh, root assemblages were strongly dominated by a single dark septate sterile fungus, also prevalent in other neighboring salt marshes. Interestingly, although its occurrence was positively correlated to soil salinity, in vitro assays revealed a strong inhibition of its growth by salts. Our results suggest that host lifestyle and soil characteristics have a strong effect on endophytic fungi and that environmental stress may entail tight plant-fungus relationships for adaptation to unfavorable conditions.

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Section: Discussioncontrasting

confidence: 99%

Fungal Assemblages Associated with Roots of Halophytic and Non-halophytic Plant Species Vary Differentially Along a Salinity Gradient

et al. 2012

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“…were the dominant taxa observed. These species were also reported to be dominant endophytes in other halophytes growing in different geographical locations, such as Egypt, India, China and South Korea (El-Morsy 2000;Sun et al 2011;You et al 2012;Kannan et al 2014). The isolation of these genera from multiple host halophytes growing in distant continents suggests that they are host-generalists.…”

Section: Discussionmentioning

confidence: 99%

Impact of a natural soil salinity gradient on fungal endophytes in wild barley (Hordeum maritimum With.)

Hammami

Baptista

Martins

et al. 2016

World J Microbiol Biotechnol

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Occurrence and distribution pattern of fungal endophytes in different tissues of halophytic plants across saline depressions are poorly studied. We investigated the endophytic fungal communities inhabiting roots, stems and leaves of Hordeum maritimum collected in a soil salinity gradient, i.e. non-saline, slightly saline and saline, using a culture-dependent approach. A total of 20 taxa belonging to Ascomycota phylum were identified by ITS rRNA gene sequence. Pyronema domesticum and Alternaria spp. were the most frequently isolated. Roots host higher diversity and were more frequently colonized by endophytes than aboveground organs. Endophytic composition of all organs surveyed differed according to salinity gradient. Contrary to expectations, the colonization rate of roots increased with soil salinity, indicating that under salt stress the endophyte-plant association is promoted. All the isolates exhibited in vitro saline tolerance, especially those belonging to genera Xylaria, Chalastospora, Alternaria and Pyronema. Fungal tolerance to NaCl under in vitro conditions appears to be more dependent on the isolates than on the sites of their isolation, suggesting that under natural conditions other factors, beyond soil salinity, should be taken into account. These findings highlight the importance of fungal endophytes in the protection and/or adaptation of both interacting species (plant-fungus) to salt stress under natural conditions.

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“…Todos os gêneros em que os isolados fúngicos apresentaram similaridade (Cladosporium, Mycosphaerella, Phaeoacremonium e Leptosphaeria) são comumente reportados por possuírem espécies fitopatogênicas (EL-MORSY, 2000;CROUS et al, 2006;APTROOT, 2006;MÁRQUEZ et al, 2010) e, também, espécies de saprófitos de solo e endofíticos (PETERSON et al, 2008), o que mostra que estes gêneros são ubíquos, apresentam habilidade na ocupação de nichos ecológicos diversos e são considerados endofíticos com múltiplos hospedeiros TRAPPE, 1998). Por outro Tabela -Percentagem de colonização das raízes de Oryza glumaepatula e Oryza sativa inoculadas com diferentes isolados de DSEF sob condições controladas 1 Médias seguidas da mesma letra minúscula nas colunas e maiúscula nas linhas, dentro de hifas ou microescleródios, não diferem significativamente ao nível de 1% de probabilidade pelo teste t de Student.…”

Section: (Aumento De 40x) (D) Hifas (H) E Microescleródios (M)unclassified

“…Em geral, tanto o isolamento quanto o cultivo de DSEF são feitos em meios batata-dextrose-ágar (HUO; GUO, 2009;YUAN et al, 2010) e em meio ágar malte (UPSON et al, 2009;PEREIRA et al, 2011;SILVANI et al, 2008). Por outro lado, os métodos mais utilizados para a preservação incluem: sílica gel (GENTLE; SCOTT, 1979), nitrogênio líquido (STALPERS et al, 1987), congelamento a -70 o C (PASSARELL; MCGINNIS, 1994), liofilização (RYBNIKAR, 1995), óleo mineral (NEUFELD; SARQUIS, 2003), água destilada (CASTELANI, 1967) e solução salina com óleo mineral (BRILHANTE et al, 2004).…”

Section: Introductionunclassified

Isolamento, armazenamento e determinação da colonização por fungos “dark septate” a partir de plantas de arroz

Ribeiro¹,

Pereira²,

Mosqueira³

et al. 2011

AGRO@MBIENTE

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Resumo -Os fungos endofíticos conhecidos como DSEF ou "dark septate" podem ser promotores de crescimento vegetal. Com o objetivo de aperfeiçoar uma metodologia para o isolamento, armazenamento e determinação da colonização de raízes de arroz por fungos "dark septate" desenvolveu-se um estudo a partir da obtenção destes microrganismos em plantas de Oryza glumaepatula coletadas em ambientes amazônicos. As raízes das plantas de doze amostras de O. glumaepatula foram seccionadas, desinfestadas e dispostas em placas com o meio ágar-malte adicionado de cloranfenicol, sulfametazol e trimetropina. Os isolados obtidos foram avaliados quanto às características de "dark septate" e armazenados em água destilada com e sem a adição de glicerol. Cinco isolados fúngicos considerados "dark septate" foram reinoculados em plantas de O. glumaepatula e em O. sativa e, também, caracterizados molecularmente através da amplificação e sequenciamento da região ITS1-5.8S-ITS2. O meio de cultura ágar-malte foi eficiente para a obtenção de "dark septate", obtendo-se mais de 50 isolados. O armazenamento em água destilada e em água destilada adicionada de glicerol mostrou-se eficiente para a manutenção das culturas fúngicas por pelo menos 12 meses. Os cinco isolados fúngicos reinoculados foram capazes de colonizar tanto raízes O. glumaepatula quanto O. sativa, não desencadeando sintomas de patogenicidade. A análise molecular mostrou que estes fungos apresentam baixa similaridade entre eles e não possuem similaridade com fungos "dark septate" já descritos, indicando que a metodologia empregada permitiu a obtenção de espécies fúngicas diversas e que a espécie O. glumaepatula abriga alta diversidade deste grupo de fungos. Palavras-Chave -Amazônia. DSEF. Oryza glumaepatula. Oryza sativa.Abstract -Endophyte fungi known as DSEF can be plant-growth-promoting. Aiming to improve a methodology for the isolation, storage and determine the rice roots colonization by "dark septate", a study was developed based on the achievement of these microorganisms in Oryza glumaepatula plants collected in Amazon region. The plant roots of twelve O. glumaepatula samples were sectioned, disinfected, and placed in plates with agar-malte medium added of chloramphenicol, sulphametazole and trimetropin. The isolates were evaluated in relation of dark septate characteristics and stored in distilled water, and in distilled water added of glycerol. Five isolates considered dark septate were re-inoculated in O. glumaepatula and O. sativa, and also analyzed through amplification and sequencing of ITS1-5.8S-ITS2 region. The agar-malte medium was efficient to isolate dark septate, and more than 50 isolates were obtained. The distilled water and also distilled water added of glycerol were efficient to maintain the fungus culture at least 12 months. The five isolates re-inoculated were able to colonize both O. glumaepatula and O. sativa, without pathogenic symptoms observed. The molecular approach reveled that these fungus are not similar to each other and also to others dark septa...

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Microfungi from the Ectorhizosphere-Rhizoplane Zone of Different Halophytic Plants from the Red Sea Coast of Egypt

Cited by 19 publications

References 0 publications

Fungal Assemblages Associated with Roots of Halophytic and Non-halophytic Plant Species Vary Differentially Along a Salinity Gradient

Fungal Assemblages Associated with Roots of Halophytic and Non-halophytic Plant Species Vary Differentially Along a Salinity Gradient

Impact of a natural soil salinity gradient on fungal endophytes in wild barley (Hordeum maritimum With.)

Isolamento, armazenamento e determinação da colonização por fungos “dark septate” a partir de plantas de arroz

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