Isolamento, armazenamento e determinação da colonização por fungos “dark septate” a partir de plantas de arroz

Ribeiro, Karen Gonçalves; Pereira, Gilmara Maria Duarte; Mosqueira, C. A.; Baraúna, Alexandre Cardoso; Vital, Marcos José Salgado; Silva, Krisle; Zilli, Jerri Édson

doi:10.18227/1982-8470ragro.v5i2.535

Cited by 12 publications

(16 citation statements)

References 21 publications

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“…The dark septate endophytic fungi present in the root fragments were identified based on the melanized and septate hyphae (RIBEIRO et al, 2011). The classification of the occurrence of this endophytic fungus was: (-) absence and (+) presence of endophytic.…”

Section: Methodsmentioning

confidence: 99%

“…The dark septate endophytic fungi characterized by the presence of septate and melanized hyphae may stimulate the growth of certain plant species by increasing the availability of nutrients (RIBEIRO et al, 2011), as well as promoting the tolerance of plants to high temperature and water stress (BARROW; OSUNA, 2002), conditions frequently found in the Northeastern region of Brazil. Santos and Varavallo (2011) also reported the induction of the plant defense system against certain pathogens by dark septate endophytic fungi, which can reduce pesticide use.…”

Section: Introductionmentioning

confidence: 99%

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NATIVE MICROBIOTA AND ARBUSCULAR MYCORRHIZAL FUNGI ON GROWTH OF Paspalum millegrana SCHRAD

et al. 2019

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Paspalum millegrana grass is a member of the family Poaceae native to the Americas, whose interaction with native symbiotic fungi has not yet been reported. The objective of this study was to evaluate the interactions between the native microorganisms and arbuscular mycorrhizal fungi in the development of P. millegrana Schrad. The experimental design was completely randomized with seven treatments (control, without AMF; native microbial inoculant; native + UFLA05 Gigaspora albida; native + UFLA351 Rhizoglomus clarum; native + UFLA372 Claroideoglomus etunicatum; native + UFLA401 Acaulospora morrowiae, and a mix of all treatments). The substrate was autoclaved sand and coconut powder at 2:1, with eight repetitions. The variables analyzed were: mycorrhizal colonization, dark septate endophytic fungi colonization, number of mycorrhizal spores, dry shoot mass, dry root mass, root length and volume, number of tiller and mycorrhizal dependence. Mycorrhizal arbuscular fungi and dark septate endophytic fungi colonized P. millegrana. The sporulation of arbuscular mycorrhizal fungi associated with P. millegrana was influenced by mycorrhizal colonization, depending on the fungus-plant interaction. P. millegrana was responsive to native + UFLA05 and native + UFLA351. No correlation between tiller emergence and mycorrhizal colonization of P. millegrana was observed. Keywords:Poaceae. Soil microbiology. Endophytic fungi. MICROBIOTA NATIVA E FUNGOS MICORRÍZICOS ARBUSCULARES NO CRESCIMENTO DEPaspalum millegrana SCHRAD RESUMO -Paspalum millegrana é uma poácea nativa das Américas, cuja interação com fungos simbiontes nativos ainda não pode ser encontrada na literatura. O objetivo deste trabalho foi avaliar a interação entre microrganismos nativos e fungos micorrízicos arbusculares no crescimento de P. millegrana Schrad. O delineamento experimental utilizado foi inteiramente casualizado composto por sete tratamentos (controle, sem inoculação; inoculante microbiano nativo; nativo + UFLA05 Gigaspora albida; nativo + UFLA351 Rhizoglomus clarum; nativo + UFLA372 Claroideoglomus etunicatum; nativo + UFLA401 Acaulospora morrowiae e mistura de todos tratamentos), em substrato à base de areia autoclavada e pó de coco, na proporção de 2:1, com oito repetições. As variáveis analisadas foram: colonização micorrízica e por fungos endofíticos, número de esporos micorrízicos, matéria seca da parte área e da raiz, comprimento e volume da raiz, número de perfilhos e dependência micorrízica. O P. millegrana foi colonizado por fungos micorrízicos arbusculares e por fungo endofítico "dark septate". A esporulação de fungos micorrízicos arbusculares associados ao P. millegrana foi influenciada pela colonização micorrízica, a depender da interação fungoplanta. O P. millegrana foi responsivo a inoculante nativo + UFLA05 (Gigaspora albida) e nativo + UFLA351 (Rhizoglomus clarum). Não houve correlação entre a emissão de perfilhos e a colonização micorrízica. Palavras-chave:Poaceae. Microbiologia do solo. Fungos endofíticos.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NATIVE MICROBIOTA AND ARBUSCULAR MYCORRHIZAL FUNGI ON GROWTH OF Paspalum millegrana SCHRAD

et al. 2019

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“…The nodulation rate (TNOD) of the nitrogen-fixing bacteria in the mycorrhizal treatments was evaluated in relation to the control, by equation 3: , where NODmp is the number of mycorrhizal plant nodules, and NODcp is the number of control plant nodules. The presence of an endophytic dark septate was calculated as a percentage in relation to the number of root fragments analyzed, as previously described by Ribeiro et al (2011).…”

Section: Methodsmentioning

confidence: 99%

“…The endophytic microorganisms present in the leaf tissue of the gliricidia from which the rhizospheric soil was collected to be used as a native inoculant, were isolated according to the methodology of Ribeiro et al (2011), with some modifications. The endophytes were isolated in a commercial potato-dextrose-agar medium (39.0 g L -1 ), after disinfestation by immersing the leaf fragments (0.25 mm 2 ) in alcohol for 1 minute, followed by immersion in 0.1% sodium hypochlorite…”

Section: Methodsmentioning

confidence: 99%

“…In addition, these authors point out that some endophytes may favor the survival of plants under conditions of drought, salinity, and / or induce resistance to phytopathogens. Among these microorganisms, the endophytic dark septate fungi, characterized by the presence of a septate and a melanized hyphae, are able to promote growth, inhibit phytopathogenic agents (RIBEIRO et al, 2011;YAN et al, 2015), and influence the mycorrhizal-plant symbiosis. Therefore, depending on microbial interaction and environmental conditions, all these factors may contribute to the host plant's growth, (LINGFEI; ANNA; ZHIWEI, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Microbial Interactions in the Development of the Biomass of Gliricidia

et al. 2018

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The objective of this work was to evaluate the interaction of microorganisms and phosphorus dosages in the development of gliricidia. The experimental design was completely randomized with six treatments (control, native microbial inoculant, and four arbuscular mycorrhizal fungi isolates: UFLA05 -Gigaspora albida, UFLA351 -Rhizoglomus clarum, UFLA372 -Claroideoglomus etunicatum, and UFLA401 -Acaulospora morrowiae), with four replicates. The parameters evaluated were: height plant, the number of branches, shoot and root dry mass matter, root length and volume, leaf phosphorus, mycorrhizal colonization, the number of spores of the arbuscular mycorrhizal fungi, the number of nodules of nitrogen fixing bacteria, and the presence of endophytic dark septate fungi, after 95 days of inoculation. The high mycorrhizal colonization of gliricidia does not guarantee an increase in biomass, which depends on the interaction of the arbuscular mycorrhizal fungi, the endophytic dark septate fungi, the nitrogen fixing bacteria, and the endophytic bacteria. Gliricidia was responsive to the inoculation of the native microbiota, UFLA372 and UFLA401. Mycorrhizal colonization by UFLA401 was influenced by the presence of nitrogen fixing bacteria. Gliricidia was not responsive to the inoculation of UFLA05 and UFLA351. The presence of the endophytic dark septate fungi did not inhibit mycorrhization and the formation of nodules of nitrogen fixing bacteria in gliricidia. Keywords:Fabaceae. Arbuscular mycorrhizal fungi. Nitrogen-fixing bacteria. Endophytic. INTERAÇÃO MICROBIANA NO DESENVOLVIMENTO DA BIOMASSA DE GLIRICÍDIARESUMO -O objetivo deste trabalho foi avaliar a interação de micro-organismos no desenvolvimento da gliricídia. O delineamento experimental utilizado foi inteiramente aleatorizado composto por seis tratamentos (controle; inoculante microbiano nativo e quatro isolados fúngicos micorrízicos arbusculares: UFLA05 -Gigaspora albida, UFLA351 -Rhizoglomus clarum, UFLA372 -Claroideoglomus etunicatum e UFLA401 -Acaulospora morrowiae), com quatro repetições. Os parâmetros avaliados foram: altura da planta, número de ramos, massa da matéria seca da parte aérea e da raiz, comprimento e volume de raiz, teor de fósforo foliar, colonização micorrízica, número de esporos de fungos micorrízicos arbusculares, número de nódulos de bactérias fixadoras de nitrogênio, presença de fungos endofíticos "dark septate", após 95 dias da inoculação. A elevada colonização micorrízica da gliricídia não garante incremento da biomassa, a depender da interação de fungos micorrízicos arbusculares, de fungos endofíticos "dark septate", de bactérias fixadoras do nitrogênio e de bactérias endofíticas. A gliricídia foi responsiva à inoculação da microbiota nativa, UFLA372 e UFLA401. A colonização micorrízica por UFLA401 foi influenciada pela presença de bactérias fixadoras do nitrogênio. A gliricídia não foi responsiva à inoculação de UFLA05 e UFLA351. A ocorrência de fungos endofíticos "dark septate" não inibiu a micorrização e a formação de nódulos de bactérias f...

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Dark septate endophyte decreases stress on rice plants

Santos

Silva

García

et al. 2017

Brazilian Journal of Microbiology

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Abiotic stress is one of the major limiting factors for plant development and productivity, which makes it important to identify microorganisms capable of increasing plant tolerance to stress. Dark septate endophytes can be symbionts of plants. In the present study, we evaluated the ability of dark septate endophytes isolates to reduce the effects of water stress in the rice varieties Nipponbare and Piauí. The experiments were performed under gnotobiotic conditions, and the water stress was induced with PEG. Four dark septate endophytes were isolated from the roots of wild rice (Oryza glumaepatula) collected from the Brazilian Amazon. Plant height as well as shoot and root fresh and dry matter were measured. Leaf protein concentrations and antioxidant enzyme activity were also estimated. The dark septate endophytes were grown in vitro in Petri dishes containing culture medium; they exhibited different levels of tolerance to salinity and water stress. The two rice varieties tested responded differently to inoculation with dark septate endophytes. Endophytes promoted rice plant growth both in the presence and in the absence of a water deficit. Decreased oxidative stress in plants in response to inoculation was observed in nearly all inoculated treatments, as indicated by the decrease in antioxidant enzyme activity. Dark septate endophytes fungi were shown to increase the tolerance of rice plants to stress caused by water deficiency.

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Isolamento, armazenamento e determinação da colonização por fungos “dark septate” a partir de plantas de arroz

Cited by 12 publications

References 21 publications

NATIVE MICROBIOTA AND ARBUSCULAR MYCORRHIZAL FUNGI ON GROWTH OF Paspalum millegrana SCHRAD

NATIVE MICROBIOTA AND ARBUSCULAR MYCORRHIZAL FUNGI ON GROWTH OF Paspalum millegrana SCHRAD

Microbial Interactions in the Development of the Biomass of Gliricidia

Dark septate endophyte decreases stress on rice plants

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