Microfluidics: Rapid Softlithography Using 3D‐Printed Molds (Adv. Mater. Technol. 10/2019)

Bazaz, Sajad Razavi; Kashaninejad, Navid; Azadi, Shohreh; Patel, Kamal; Asadnia, Mohsen; Jin, Dayong; Warkiani, Majid Ebrahimi

doi:10.1002/admt.201970056

Cited by 1 publication

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“…As the cost of DLP 3D printing technology decreases and its precision improves, it has now become a highly accessible microfabrication method, capable of low-cost, rapid production of templates for microwell arrays, and microchannel chips. 18,19 Despite its vast potential, there has not been systematic research utilizing 3D printing to develop liquid dispensing methods or characterize their accuracy and precision. We discovered that the microarray from 3D printed templates possesses high volumetric accuracy, facilitating the rapid creation of microchambers ranging from nanoliters to microliters in volume.…”

Section: ■ Introductionmentioning

confidence: 99%

Microarray Platforms Based on 3D Printing

Qin,

Qian,

Lai

et al. 2024

Anal. Chem.

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This paper introduces an innovative method for the fabrication and infusion of microwell arrays based on digital light processing (DLP) 3D printing. A low-cost DLP 3D printer is employed to fabricate microstructures rapidly with a broad dynamic range while maintaining high precision and fidelity. We constructed microwell arrays with varying diameters, from 200 to 2000 μm and multiple aspect ratios, in addition to microchannels with widths ranging from 45 to 1000 μm, proving the potential and flexibility of this fabrication method. The superimposition of parallel microchannels onto the microwell array, facilitated by positive or negative pressure, enabled the transfer of liquid to the microwells. Upon removal of the microchannel chip, a dispensed microdroplet array was obtained. This array can be modulated by adjusting the volume of the microwells and the inflow fluid. The filled microwell array allows chip-to-chip dispensing to the microreactor array through binding and centrifugation, facilitating multistep and multireagent assays. The 3D printing approach also enables the fabrication of intricate cavity designs, such as micropyramid arrays, which can be integrated with parallel microchannels to generate spheroid flowcells. This device demonstrated the ability to generate spheroids and manipulate their environment. We have successfully utilized precise modulation of spheroids size and performed parallel drug dose− response assays to evaluate its effectiveness. Furthermore, we managed to execute dynamic drug combinations based on a compact spheroids array, utilizing two orthogonal parallel microchannels. Our findings suggest that both the combination and temporal sequence of drug administration have a significant impact on therapeutic outcomes.

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Section: ■ Introductionmentioning

confidence: 99%