Microfluidics for Neuronal Cell and Circuit Engineering

Habibey, Rouhollah; Arias, Jesús Eduardo Rojo; Striebel, Johannes; Busskamp, Volker

doi:10.1021/acs.chemrev.2c00212

Cited by 26 publications

(33 citation statements)

References 480 publications

(1,031 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Once sealed against a glass coverslip, the microgrooves formed microchannels [ 31 , 32 ]. The resulting devices were developed in many ways by multiple research groups to compartmentalize and study neurons as well as neuronal networks in various physiological and pathological contexts [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Impact of Neurons on Patient-Derived Cardiomyocytes Using Organ-On-A-Chip and iPSC Biotechnologies

Bernardin

Colombani

Rousselot

et al. 2022

Cells

View full text Add to dashboard Cite

In the heart, cardiac function is regulated by the autonomic nervous system (ANS) that extends through the myocardium and establishes junctions at the sinus node and ventricular levels. Thus, an increase or decrease in neuronal activity acutely affects myocardial function and chronically affects its structure through remodeling processes. The neuro–cardiac junction (NCJ), which is the major structure of this system, is poorly understood and only a few cell models allow us to study it. Here, we present an innovant neuro–cardiac organ-on-chip model to study this structure to better understand the mechanisms involved in the establishment of NCJ. To create such a system, we used microfluidic devices composed of two separate cell culture compartments interconnected by asymmetric microchannels. Rat PC12 cells were differentiated to recapitulate the characteristics of sympathetic neurons, and cultivated with cardiomyocytes derived from human induced pluripotent stem cells (hiPSC). We confirmed the presence of a specialized structure between the two cell types that allows neuromodulation and observed that the neuronal stimulation impacts the excitation–contraction coupling properties including the intracellular calcium handling. Finally, we also co-cultivated human neurons (hiPSC-NRs) with human cardiomyocytes (hiPSC-CMs), both obtained from the same hiPSC line. Hence, we have developed a neuro–cardiac compartmentalized in vitro model system that allows us to recapitulate the structural and functional properties of the neuro–cardiac junction and that can also be used to better understand the interaction between the heart and brain in humans, as well as to evaluate the impact of drugs on a reconstructed human neuro–cardiac system.

show abstract

Section: Resultsmentioning

confidence: 99%

Impact of Neurons on Patient-Derived Cardiomyocytes Using Organ-On-A-Chip and iPSC Biotechnologies

Bernardin

Colombani

Rousselot

et al. 2022

Cells

View full text Add to dashboard Cite

show abstract

“…Once sealed against a glass coverslip microgroove formed microchannels [31, 32]. The resulting devices were developed in many ways by multiples research groups to compartmentalize and study neurons as well as neuronal networks in various physiological and pathological contexts [33].…”

Section: Resultsmentioning

confidence: 99%

Impact of neurons on patient derived-cardiomyocytes using organ-on-a-chip and iPSC biotechnologies

Bernardin

Colombani

Rousselot

et al. 2022

Preprint

View full text Add to dashboard Cite

In the heart, cardiac function is regulated by the autonomic nervous system (ANS) that extends through the myocardium and establish junctions at the sinus node and ventricular levels. Thus, an increase or decrease of neuronal activity acutely affects myocardial function and chronically affects its structure through remodeling processes. The neuro-cardiac junction (NCJ), which is the major structure of this system, is poorly understood and only few cell models allow us to study it. Here we present an innovant neuro-cardiac organ-on-chip model to study this structure to better understand the mechanisms involved in the establishment of NCJ. To create such a system, we used microfluidic devices composed of two separate cells compartment interconnected by asymmetric microchannels. Rat PC12 cells, were differentiated to recapitulate the characteristics of sympathetic neurons, and cultivated with cardiomyocytes derived from human induced pluripotent stem cells (hiPSC). We confirmed the presence of specialized structure between the two cell types that allow neuromodulation and observed that the neuronal stimulation impacts the excitation-contraction coupling properties including the intracellular calcium handling. Finally, we also co-cultivated human neurons (hiPSC-NRs) with human cardiomyocytes (hiPSC-CMs) both obtained from the same hiPSC line. Hence, we have developed a neuro-cardiac compartmentalized in vitro model system that allows to recapitulate structural and functional properties of neuro-cardiac junction and that can be used to better understand interaction between heart and brain in humans, as well as to evaluate the impact of drugs on a reconstructed human neuro-cardiac system.

show abstract

“…Furthermore, the microfluidic platform allows the analysis of dynamic cell–cell interactions under a reproducible in vitro culture condition. In recent years, microfluidic systems have been developed for a wide range of applications in cancer research, drug screening, vascular models, and neuroscience [ 67 ].…”

Section: Microfluidic Platform-based Neural-glial Cell Co-culture Systemmentioning

confidence: 99%

From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction

Liu

Meng

et al. 2022

IJMS

View full text Add to dashboard Cite

The central nervous system (CNS) controls and regulates the functional activities of the organ systems and maintains the unity between the body and the external environment. The advent of co-culture systems has made it possible to elucidate the interactions between neural cells in vitro and to reproduce complex neural circuits. Here, we classified the co-culture system as a two-dimensional (2D) co-culture system, a cell-based three-dimensional (3D) co-culture system, a tissue slice-based 3D co-culture system, an organoid-based 3D co-culture system, and a microfluidic platform-based 3D co-culture system. We provide an overview of these different co-culture models and their applications in the study of neural cell interaction. The application of co-culture systems in virus-infected CNS disease models is also discussed here. Finally, the direction of the co-culture system in future research is prospected.

show abstract

Microfluidics for Neuronal Cell and Circuit Engineering

Cited by 26 publications

References 480 publications

Impact of Neurons on Patient-Derived Cardiomyocytes Using Organ-On-A-Chip and iPSC Biotechnologies

Impact of Neurons on Patient-Derived Cardiomyocytes Using Organ-On-A-Chip and iPSC Biotechnologies

Impact of neurons on patient derived-cardiomyocytes using organ-on-a-chip and iPSC biotechnologies

From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction

Contact Info

Product

Resources

About