Microfluidics for Gametes, Embryos, and Embryonic Stem Cells

Smith, Gary D.; Swain, Jason E.; Bormann, Charles L.

doi:10.1055/s-0030-1268699

Cited by 31 publications

(20 citation statements)

References 51 publications

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“…In other words, microfluidic technology tailors the cellular microenvironment as pointed out by Young and Beebe (70). Microfluidic technology promises to expand our perspective regarding gamete and embryo culture as it holds revolutionary potential (71). Challenges of accommodating new ideas and technologies seem to be a mandatory process for establishing a better future for IVF practice.…”

Section: Latest Trendsmentioning

confidence: 99%

Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics

Simopoulou

Sfakianoudis²,

Rapani

et al. 2018

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Section: Latest Trendsmentioning

confidence: 99%

Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics

Simopoulou

Sfakianoudis²,

Rapani

et al. 2018

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“…) or to harvest embryos (Smith et al. ). Showing that there are a vast range of techniques that must be explored to better and efficiently enable in vitro production of sperm cells.…”

Section: Culture Techniques For Improving In Vitro Sperm Productionmentioning

confidence: 99%

A Review of New Technologies that may Become Useful for in vitro Production of Boar Sperm

Gadella

Ferraz

2015

Reprod Domestic Animals

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Contents Making sperm cells outside the original testicular environment in a culture dish has been considered for a long time as impossible due to the very complicated process of spermatogenesis and sperm maturation, which altogether, encompasses a 2‐month period. However, new approaches in complex three‐dimensional co‐cell cultures, micro‐perfusion and micro‐fluidics technologies, new knowledge in the functioning, culturing and differentiation of spermatogonial stem cells (SSC) and their precursor cells have revolutionized this field. Furthermore, the use of better molecular markers as well as stimulatory factors has led to successful in vitro culture of stem cells either derived from germ line stem cells, from induced pluripotent stem cells (iPSC) or from embryonic stem cells (ESC). These stem cells when placed into small seminiferous tubule fragments are able to become SSC. The SSC beyond self‐renewal can also be induced into haploid sperm‐like cells under in vitro conditions. In mouse, this in vitro produced sperm can be injected into a mature oocyte and allow post‐fertilization development into an early embryo in vitro. After transferring such obtained embryos into the uterus of a recipient mouse, they can further develop into healthy offspring. Recently, a similar approach has been performed with combining selected cells from testicular cell suspensions followed by a complete in vitro culture of seminiferous cords producing sperm‐like cells. However, most of the techniques developed are laborious, time‐consuming and have low efficiency, placing questionable that it will become useful used for setting up an efficient in vitro sperm production system for the boar. The benefits and drawbacks as well as the likeliness of in vitro pig sperm production to become applied in assisted technologies for swine reproduction are critically discussed. In this contribution, also the process of sperm production in the testis and sperm maturation is reviewed.

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“…Krisher et al [289], have reviewed the advantages of microfluidics for in vitro embryo production. Other reviews have dealt with assisted reproduction technology and embryonic stem cell growth and differentiation [29,290].…”

Section: E Colimentioning

confidence: 99%

“…Zhu et al [26] has described developments in microbioreactors up until 2005, covering aspects of designs, operation and application. Most of the research has focused on applications in: (i) microbial bioprocessing [27][28] (ii) stem cells [29][30] (iii) single cells [31] (iv) drug development [32] and (v) cytotoxicity [33]. In this review we consider some basic concepts, designs and operational requirements for microbioreactors for cell-based studies.…”

Section: Introductionmentioning

confidence: 99%