Microfluidics and point-of-care testing

Sia, Samuel K.; Kricka, Larry J.

doi:10.1039/b817915h

Cited by 246 publications

(153 citation statements)

References 3 publications

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“…133 Another rapidly emerging development in microfluidics in a lab-on-a-chip approach aims to develop POC devices to improve diagnostic capacity, and is already is being optimized for implementation for sexually transmitted infections and HIV-1 detection. 134 …”

Section: Technologies In Developmentmentioning

confidence: 99%

Malaria Diagnostics in Clinical Trials

Murphy¹,

Shott²,

Parikh³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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Abstract. Malaria diagnostics are widely used in epidemiologic studies to investigate natural history of disease and in drug and vaccine clinical trials to exclude participants or evaluate efficacy. The Malaria Laboratory Network (MLN), managed by the Office of HIV/AIDS Network Coordination, is an international working group with mutual interests in malaria disease and diagnosis and in human immunodeficiency virus/acquired immunodeficiency syndrome clinical trials. The MLN considered and studied the wide array of available malaria diagnostic tests for their suitability for screening trial participants and/or obtaining study endpoints for malaria clinical trials, including studies of HIV/ malaria co-infection and other malaria natural history studies. The MLN provides recommendations on microscopy, rapid diagnostic tests, serologic tests, and molecular assays to guide selection of the most appropriate test(s) for specific research objectives. In addition, this report provides recommendations regarding quality management to ensure reproducibility across sites in clinical trials. Performance evaluation, quality control, and external quality assessment are critical processes that must be implemented in all clinical trials using malaria tests.

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Section: Technologies In Developmentmentioning

confidence: 99%

Malaria Diagnostics in Clinical Trials

Murphy¹,

Shott²,

Parikh³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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“…In fact, this technological platform allows obtaining significant results on over expanding fields, going from medicine 1,2 to biology, and from environmental monitoring 3 to aerospace applications. 4 The development of more complex and effective microfluidic devices strictly depends on the possibility to address challenging needs, such as miniaturized and light devices, low consumption of samples and reagents, complex sample preparation, short time-to-result, and simple readout.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration

et al. 2015

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The flow focusing is a fundamental prior step in order to sort, analyze, and detect particles or cells. The standard hydrodynamic approach requires two fluids to be injected into the microfluidic device: one containing the sample and the other one, called the sheath fluid, allows squeezing the sample fluid into a narrow stream. The major drawback of this approach is the high complexity of the layout for microfluidic devices when parallel streams are required. In this work, we present a novel parallelized microfluidic device that enables hydrodynamic focusing in each microchannel using a single feed flow. At each of the parallel channels, a cross-filter region is present that allows removing fluid from the sample fluid. This fluid is used to create local sheath fluids that hydrodynamically pinch the sample fluid. The great advantage of the proposed device is that, since only one inlet is needed, multiple parallel micro-channels can be easily introduced into the design. In the paper, the design method is described and the numerical simulations performed to define the optimal design are summarized. Moreover, the operational functionality of devices tested by using both polystyrene beads and Acute Lymphoid Leukemia cells are shown. V C 2015 AIP Publishing LLC.

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“…However, in spite of these economic and scientific benefits, microfluidic devices have been relatively slow to propagate into actual applications, especially in the field of point-of-care diagnostics and some resource-limited applications or environments. 8,9 One reason for this could be that these devices typically require bulky and expensive external hardware to initiate and control fluid flow. At present, traditional pumps such as syringe pumps, peristaltic pumps, and regulated source of pressure or vacuum are widely utilized in microfluidic systems.…”

Section: Introductionmentioning

confidence: 99%

A “place n play” modular pump for portable microfluidic applications

Li¹,

Luo²,

Chen³

et al. 2012

Biomicrofluidics

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This paper presents an easy-to-use, power-free, and modular pump for portable microfluidic applications. The pump module is a degassed particle desorption polydimethylsiloxane (PDMS) slab with an integrated mesh-shaped chamber, which can be attached on the outlet port of microfluidic device to absorb the air in the microfluidic system and then to create a negative pressure for driving fluid. Different from the existing monolithic degassed PDMS pumps that are generally restricted to limited pumping capacity and are only compatible with PDMS-based microfluidic devices, this pump can offer various possible configures of pumping power by varying the geometries of the pump or by combining different pump modules and can also be employed in any material microfluidic devices. The key advantage of this pump is that its operation only requires the user to place the degassed PDMS slab on the outlet ports of microfluidic devices. To help design pumps with a suitable pumping performance, the effect of pump module geometry on its pumping capacity is also investigated. The results indicate that the performance of the degassed PDMS pump is strongly dependent on the surface area of the pump chamber, the exposure area and the volume of the PDMS pump slab. In addition, the initial volume of air in the closed microfluidic system and the cross-linking degree of PDMS also affect the performance of the degassed PDMS pump. Finally, we demonstrated the utility of this modular pumping method by applying it to a glass-based microfluidic device and a PDMS-based protein crystallization microfluidic device. V C 2012 American Institute of Physics. [http://dx

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Microfluidics and point-of-care testing

Cited by 246 publications

References 3 publications

Malaria Diagnostics in Clinical Trials

Malaria Diagnostics in Clinical Trials

Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration

A “place n play” modular pump for portable microfluidic applications

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