Microfluidic technologies for cell deformability cytometry

Abstract

Microfluidic detection methods for cell deformability cytometry have been regarded as powerful tools for single‐cell analysis of cellular mechanical phenotypes, thus having been widely applied in the fields of cell preparation, separation, clinical diagnostics and so on. Featured with traits like easy operations, low cost and high throughput, such methods have shown great potentials on investigating physiological state and pathological changes during cellular deformation. Herein, a review on the advancements of microfluidic‐based cell deformation cytometry is presented. We discuss several representative microfluidic‐based cell deformability cytometry methods with their frontiers in practical applications. Finally, we analyze the current status and propose the remaining challenges with future perspectives and development directions.

Ultrasound-responsive matters for biomedical applications

“…Since PFC liquid features a low boiling point, microfluidic technology allows a moderate fabrication process, thus realizing the maintenance of the physiochemical properties of PFC. 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 Ascribed to the US-induced phase-transition ability of PFCs, the PO/GI-MCs experienced the transition from microcapsules to microbubbles when exposed to US waves (1.0 MHz, 1.2 W cm −2 ). Since the volume of PFCs can become five times larger, the resultant gas core splits the alginate shell, releasing the gas, which contains a large amount of oxygen.…”

Emerging diagnostic and therapeutic technologies based on ultrasound-triggered biomaterials

“…Thus, efforts have been made to enhance the amount of oxygen during SDT against tumors. Based on the precise controllability and maneuverability of microfluidic technology [92][93][94][95], our group proposed a multi-drug co-loaded microcarrier, whose shell carries chemotherapeutics and sonosensitizer, while core loading oxygen-enriched PFC (PO/GI-MCs) (figure 11(a)) [96]. The presented PO/GI-MCs showed not only ideal tumor inhibitory effect on pancreatic cancer, but also effectively alleviated the hypoxic microenvironment at the tumor site.…”

Vein-Like Ni-BTC@Ni3S4 with Sulfur Vacancy and Ni3+ Fabricated In Situ Etching Vulcanization Strategy for an Electrochemical Sensor of Dopamine

“…In recent years, detection technologies have played an important role in clinical diagnostics, disease treatment, and anticancer drug development. , At present, the detection and analysis methods for DA mainly include high-performance liquid chromatography (HPLC), chemiluminescence, spectrophotometry, colorimetry, surface-enhanced Raman spectroscopy (SERS), and electrochemical methods . Among the above technologies, electrochemical sensing technology has attracted much attention because of its simple operation, high sensitivity, fast detection, low cost, and other advantages and has been widely applied in the detection of food, biological molecules, environment polutants, , and so on.…”