Microfluidic Sample Preparation for Single Cell Analysis

Hosic, Sanjin; Murthy, Shashi K.; Koppes, Abigail N.

doi:10.1021/acs.analchem.5b04077

Cited by 134 publications

(108 citation statements)

References 278 publications

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“…For automated drug susceptibility test, methods that can automatically isolate the cancer cells from healthy cells are required. Traditional methods for cell isolation are mainly based on fluorescent activated cell sorting (FACS) and magnetic activated cell sorting (MACS) [50]. For FACS, shear flow focuses fluorescently-labeled cells which are then excited by a laser and given a specific charge based upon the fluorescent dye color.…”

Section: Automated Cell Isolation and Deliverymentioning

confidence: 99%

“…In microfluidics with micrometer sized channels, the Reynolds number is typically small and inertial effects are often neglected. However, at modest channel dimensions or velocities, inertial effects manifest and can be exploited for cell sorting [50]. Cells with differing properties such as size, density, or shape respond differently to inertial effects and are focused at different channel locations.…”

Section: Automated Cell Isolation and Deliverymentioning

confidence: 99%

“…The advantage of acoustic method is that it is noninvasive and has no impact on the viability, function, and gene expression of cells. However, this method require wavelengths comparable to microfluidic channel width, and therefore the sorting rate cannot be increased because the wave frequency is constrained [50], resulting the low throughput. In 2013, Huang et al [63] presented a CTC isolation method by combining microfluidic with optically-induced electrophoretic (ODEP) technique, as shown in Fig.…”

Section: (D)mentioning

confidence: 99%

“…The next issues needing to be addressed are the integrations, such as the integrations between the microfluidics-based primary cell isolation system and the conveyor system, and the integrations between the conveyor system and micro/nano robots or micro/nano manipulators. It should be noted that the drawback of microfluidics is that microfluidic devices are not readily reusable due to clogging and cell adhesion [50], posing a challenge when large quantities of cells are treated. Collectively, microfluidics has the potential to work as an important building block for automated cell isolation and delivery systems, which may play an important role in micro/nano automation for personalized medicine.…”

Section: (D)mentioning

confidence: 99%

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Applications of Micro/Nano Automation Technology in Detecting Cancer Cells for Personalized Medicine

Liu

et al. 2017

IEEE Trans. Nanotechnology

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Abstract-The coming era of personalized cancer treatment is presenting automation with unprecedented opportunities. Currently, the drug susceptibility test of clinical cancer patients is mainly dependent on manual labor with a low level of automation. Automating the process of primary cancer cell detection will potentially have tremendous economic benefits and social significance. Automated cancer cell detection means developing robotic and automation equipments to handle single cells and molecules at the micro/nanometer-scale. The achievements of information science, engineering technology, life sciences, and nanotechnology in the past decades have led to the birth of robots that can perform effective manipulations on single living cells at the micro/nanometerscale in aqueous conditions, opening the door to automated cancer cell detection. However, there is still a huge gap between current single-cell micro/nano automation technology and clinical requirements for personalized medicine. In this paper, we will review the progress of single-cell micro/nano automation technology in recent years and discuss the facing challenges and future directions in three aspects, including automated cell isolation and delivery, automatically acquiring the physiological features of cells and data analysis.

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Section: Automated Cell Isolation and Deliverymentioning

confidence: 99%

Section: Automated Cell Isolation and Deliverymentioning

confidence: 99%

Section: (D)mentioning

confidence: 99%

Section: (D)mentioning

confidence: 99%

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Applications of Micro/Nano Automation Technology in Detecting Cancer Cells for Personalized Medicine

Liu

et al. 2017

IEEE Trans. Nanotechnology

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“…21 Among these, a method using a microwell array was chosen for our study on cell volume regulation of Madin-Darby canine kidney (MDCK) single cells adhered onto a surface. Microwell arrays have been widely used to spatially arrange a large number of single cells, because they can separate singles cells by physical boundaries.…”

Section: Introductionmentioning

confidence: 99%

High-throughput Screening of Erratic Cell Volume Regulation Using a Hydrogel-based Single-cell Microwell Array

Fleischauer

Heo

2017

ANAL. SCI.

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Here, we report that a single-cell microwell array based on photocrosslinked hydrogel can be used to screen cells exhibiting a defective regulatory volume decrease (RVD) in high-throughput. The RVD is a regulatory function of cells that maintains cell volume homeostasis in a hypotonic medium. Single Madin-Darby canine kidney (MDCK) cells grown in the microwells were loaded with a volume-sensitive fluorescence dye. Changes in the volume of discrete single cells were traced for 20 min in a hypotonic solution using a wide-field fluorescence microscopy. The volume changes of more than 100 single cells were analyzed simultaneously using time-lapse fluorescence micrographs. Cells showing erratic RVD could be easily screened from the image analysis. Nearly 40% of the MDCK single cells exhibited weak, or no, RVD. Since other previously reported methods could not detect as many changes in the volume of discrete singles cells as the method used in this report, we anticipate that our reported method will provide an efficient way of elucidating the RVD mechanisms of cells that have not yet been completely understood.

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Microfluidics Cell Loading‐Dock System: Ordered Cellular Array for Dynamic Lymphocyte‐Communication Study

et al. 2017

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It remains a great challenge to establish a high‐throughput platform that can explore the interactions among multiple lymphocytes (>2 cells) and retrieve the interested cells for downstream analysis. This study demonstrates a microfluidics cell loading‐dock system (Cell‐Dock) to enclose multiple cells in 1D, 2D, and 3D chambers with high throughput and efficiency and single‐cell accuracy. The loading efficiencies of 95%, 85%, and 74% for one‐, three‐, and five‐cell systems are achieved, respectively. The Cell‐Dock system provides precise and dynamic cell packing models to facilitate lymphocyte‐interaction studies. The results demonstrate that individual natural killer (NK) cells may function independently rather than cooperate to lyse target cells in the defined microenvironment. Furthermore, the strong/weak NK cells are retrieved based on their on‐chip cytotoxicity and mRNA sequencing is conducted to find the possible mechanisms for “serial killing,” an important but unsolved issue. This study finds that the stronger NK cells overexpress multiple genes involved in cytotoxicity and adhesion molecules (including the well‐known ICAM1 and seldom reported B4GALT1) might play important roles in the regulation of NK cytolysis.

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Microfluidic Sample Preparation for Single Cell Analysis

Cited by 134 publications

References 278 publications

Applications of Micro/Nano Automation Technology in Detecting Cancer Cells for Personalized Medicine

Applications of Micro/Nano Automation Technology in Detecting Cancer Cells for Personalized Medicine

High-throughput Screening of Erratic Cell Volume Regulation Using a Hydrogel-based Single-cell Microwell Array

Microfluidics Cell Loading‐Dock System: Ordered Cellular Array for Dynamic Lymphocyte‐Communication Study

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