2022
DOI: 10.1039/d2lc00006g
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Microfluidic platforms for extracellular vesicle isolation, analysis and therapy in cancer

Abstract: Extracellular vesicles (EVs) are small lipidic particles packed with proteins, DNA, messenger RNA and microRNAs of their cell of origin that act as critical players in cell-cell communication. These vesicles...

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Cited by 31 publications
(24 citation statements)
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“…However, the concentration of EVs with good potential to be disease markers in biological samples could be very low, making it very challenging to detect such EVs using conventional methods like ELISA, western blotting (WB), and flow cytometry (FCM) . State-of-the-art sensors built on microfluidic and nanofluidic devices have been developed to detect EVs down to 100 particles/mL. , Still, simple but sensitive techniques for rapid EV detection are in demand to speed up the discovery and clinical applications of EV-based disease markers.…”
Section: Introductionmentioning
confidence: 99%
“…However, the concentration of EVs with good potential to be disease markers in biological samples could be very low, making it very challenging to detect such EVs using conventional methods like ELISA, western blotting (WB), and flow cytometry (FCM) . State-of-the-art sensors built on microfluidic and nanofluidic devices have been developed to detect EVs down to 100 particles/mL. , Still, simple but sensitive techniques for rapid EV detection are in demand to speed up the discovery and clinical applications of EV-based disease markers.…”
Section: Introductionmentioning
confidence: 99%
“…[99][100][101][102] In addition, microfluidics offered efficient EV isolation candidate for fast diagnostic and analysis, but low yield might limit the further application of EVs. [84,87,103] Besides, precipitation method also provided possibility for efficient EV isolation. [88,89] The hydrophilic polymers, like poly(ethylene glycol) (PEG), could competitively bind with free water molecules surrounding EVs to create hydrophobic environment, resulting in the precipitation of EVs with relatively high yield.…”
Section: Preparation Of Ev-based Drug Delivery Systemsmentioning
confidence: 99%
“…Therefore researchers have recently started integrating different methods based on the physical properties of exosomes with the design of the microfluidic device. Thus, microfluidics-based isolation techniques have developed and evolved over the years [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. The following sections will discuss some of the most interesting methods mostly based on the immunoaffinity approaches.…”
Section: Isolation and Detection Techniquesmentioning
confidence: 99%