Microfluidic Paper‐Based Analytical Device for Sensitive Detection of Peptides Based on Specific Recognition of Aptamer and Amplification Strategy of Hybridization Chain Reaction

Ma, Chao; Liu, Haiyun; Zhang, Lina; Li, Li; Yan, Mei; Yu, Jinghua; Song, Xianrang

doi:10.1002/celc.201600824

Cited by 17 publications

(12 citation statements)

References 36 publications

(20 reference statements)

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“…Luminescence, as a general term is related to the energy transition between molecular orbitals that produces an emission of light. When the excitation of the molecules is caused by a chemical reaction, this light emission is chemiluminescence [ 27 , 33 , 36 , 58 , 71 , 81 ]. The emission that accompanies an electrochemical reaction is known as electrochemiluminescence [ 79 ].…”

Section: Detection Methods and Assay Formatsmentioning

confidence: 99%

“…Origami paper analytical devices (oPADs) have been introduced by several groups [ 79 , 80 , 81 ]. For example, Liu et al [ 80 ] used a glucose oxidase tag to modify the relative concentrations of an electroactive redox couple, and a digital multimeter (DMM) to transduce the result.…”

Section: Classification Of Microdevicesmentioning

confidence: 99%

“…The ECL intensity increased only when ATP (adenosine triphosphate) was present. On the other hand, Ma et al [ 81 ] developed the specific recognition of an aptamer and the amplification strategy of a hybridization chain reaction (HCR) using an electrochemiluminescence (ECL) probe (Ru(phen) 3 2+ ). Lateral flow strip assays (LFSAs) are another type of paper-based microdevices.…”

Section: Classification Of Microdevicesmentioning

confidence: 99%

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Recent Microdevice-Based Aptamer Sensors

et al. 2018

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Since the systematic evolution of ligands by exponential enrichment (SELEX) method was developed, aptamers have made significant contributions as bio-recognition sensors. Microdevice systems allow for low reagent consumption, high-throughput of samples, and disposability. Due to these advantages, there has been an increasing demand to develop microfluidic-based aptasensors for analytical technique applications. This review introduces the principal concepts of aptasensors and then presents some advanced applications of microdevice-based aptasensors on several platforms. Highly sensitive detection techniques, such as electrochemical and optical detection, have been integrated into lab-on-a-chip devices and researchers have moved towards the goal of establishing point-of-care diagnoses for target analyses.

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Section: Detection Methods and Assay Formatsmentioning

confidence: 99%

Section: Classification Of Microdevicesmentioning

confidence: 99%

Section: Classification Of Microdevicesmentioning

confidence: 99%

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Recent Microdevice-Based Aptamer Sensors

et al. 2018

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“…ECL is a powerful analytical technique which is gaining increased attention for the development of analytical methods using µPADs [79]. One example is the ECL-based µPAD developed by Ma et al [101]. This work uses the specific recognition by aptamers and the amplification strategy of a hybridization chain reaction (HCR) with the ECL probe, Ru(phen)3 2+ for detecting the analyte peptides.…”

Section: Chemiluminescence and Electrochemiluminescence-based Detectionmentioning

confidence: 99%

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Khan

Song

2020

Micromachines

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Aptamers are oligonucleotides or peptides that are selected from a pool of random sequences that exhibit high affinity toward a specific biomolecular species of interest. Therefore, they are ideal for use as recognition elements and ligands for binding to the target. In recent years, aptamers have gained a great deal of attention in the field of biosensing as the next-generation target receptors that could potentially replace the functions of antibodies. Consequently, it is increasingly becoming popular to integrate aptamers into a variety of sensing platforms to enhance specificity and selectivity in analyte detection. Simultaneously, as the fields of lab-on-a-chip (LOC) technology, point-of-care (POC) diagnostics, and personal medicine become topics of great interest, integration of such aptamer-based sensors with LOC devices are showing promising results as evidenced by the recent growth of literature in this area. The focus of this review article is to highlight the recent progress in aptamer-based biosensor development with emphasis on the integration between aptamers and the various forms of LOC devices including microfluidic chips and paper-based microfluidics. As aptamers are extremely versatile in terms of their utilization in different detection principles, a broad range of techniques are covered including electrochemical, optical, colorimetric, and gravimetric sensing as well as surface acoustics waves and transistor-based detection.

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“…Another ECL aptasensor was developed to detect peptides (Ma et al, 2017). Detailes of the process are as follows: the paper is modified with a thiolated capture probe (CP) via Au-S bonds.…”

Section: Electrochemiluminescence Paper-based Aptasensorsmentioning

confidence: 99%