Microfluidic module for automated isolation and purification of nucleic acids from biological samples

Khodakov, Dmitriy A.; Mamaev, D. D.; Dementieva, Ekaterina; Filatov, I. V.; Yurasov, D. A.; Cherepanov, A. I.; Vasiliskov, V. A.; Smoldovskaya, Olga; Gryadunov, Dmitry; Mikhailovich, Vladimir; Заседателев, А. С.; Makarov, Alexander А.

doi:10.1134/s1607672910060037

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…3.1 Sample processing and NA isolation procedure in disposable fluidic modules In this work, the protocol of sample preparation and NA extraction described earlier (Khodakov et al 2010;Mamaev et al 2011) was used as a basis for the development of the platform with multiple fluidic modules for automated NA isolation from several biological samples. The process involved two-step enzymatic and chemical lysis followed by SPE.…”

Section: Resultsmentioning

confidence: 99%

“…Previously, we reported on the development of the device for the isolation and purification of nucleic acids in an automated mode using an original microfluidic cartridge (Khodakov et al 2010;Mamaev et al 2011). The device allowed the isolation of nucleic acids only from a single biological sample using a combined enzymatic and chemical lysis step followed by the SPE.…”

Section: Introductionmentioning

confidence: 99%

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Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids

Mamaev

Shaskolskiy

Dementieva

et al. 2015

Biomed Microdevices

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We describe the development and evaluation of a rotary-based platform with multiple disposable fluidic modules for simultaneous automatic nucleic acid (NA) isolation from up to 24 biological samples. The procedure is performed inside insulated individual disposable modules, which minimizes both the risk of infection of personnel and laboratory cross-contamination. Each module is a segment of a circular cylinder containing a leak-proof inlet port for sample input, reservoirs with lyophilized chemicals and solvents, fluidic channels, stoppers, valves, a waste reservoir and an outlet port equipped with the standard micro test tube for NA collection. The entire platform, apart from the rotor that accommodates 24 modules, consists of functional elements that provide spinning of the rotor, reagent mixing, pressure delivery, and heating of reaction mixtures. The transfer of the reaction mixtures inside the modules is performed either with rotation of the rotor or with excessive air pressure applied to the module's reservoirs. The entire process takes less than 40 min, starting from the sample loading to the recovery of the purified NA, and it allows NA isolation both from bacterial cells and viral particles. The feasibility and reproducibility of the developed platform was demonstrated by the NA isolation from suspensions of Bacillus thuringiensis and Mycobacterium tuberculosis cells within a concentration range of 10(8) to 10(2) cells/ml. Isolation of NAs from blood plasma samples with varying concentration of hepatitis B and C viruses from 10(7) to 10(2) particles/ml were also successful. The purity and integrity of the extracted NAs were both reliable for performing quantitative PCR.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids

Mamaev

Shaskolskiy

Dementieva

et al. 2015

Biomed Microdevices

View full text Add to dashboard Cite

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Identification of plant-derived genetically modified organisms in food and feed using a hydrogel oligonucleotide microchip

et al. 2011

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Gel-based microarrays in clinical diagnostics in Russia

Gryadunov

Dementieva

Mikhailovich

et al. 2011

Expert Review of Molecular Diagnostics

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Immobilization of molecular probes in 3D hydrogel elements provides some essential advantages compared with conventional flat surfaces. In this article, an integrated technology based on the use of low-density microarrays comprised of hemispherical gel elements, developed at the Engelhardt Institute of Molecular Biology (Moscow, Russia) for various applications will be reviewed. The structure of the gel can be adapted for immobilization of virtually any biological molecules in a natural hydrophilic environment. The discrimination between matching and mismatching duplexes of nucleic acids in these conditions is more reliable than on conventional flat surfaces, minimizing the number of elements needed to detect specific sequences. Protein molecules immobilized in hydrogel-based biochips better preserve their biological properties. As described in this article, such biochips were successfully applied for laboratory diagnostics in a wide variety of clinical conditions involving the identification of bacterial and viral pathogens, cancer-related mutations and protein tumor markers.

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Microfluidic module for automated isolation and purification of nucleic acids from biological samples

Cited by 3 publications

References 12 publications

Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids

Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids

Identification of plant-derived genetically modified organisms in food and feed using a hydrogel oligonucleotide microchip

Gel-based microarrays in clinical diagnostics in Russia

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