Microfluidic isotachophoresis: A review

Šmejkal, Petr; Bottenus, Danny; Breadmore, Michael C.; Guijt, Rosanne M.; Ivory, Cornelius F.; Foret, František; Macka, Mirek

doi:10.1002/elps.201300021

Cited by 75 publications

(64 citation statements)

References 152 publications

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“…The direct manipulation of microbials by electrophoresis 49 and isotachophoresis 50 has been reviewed in prior work. The emerging interest for dielectrophoretic techniques within literature is apparent from the citation analysis in Figure 1.…”

Section: Introductionmentioning

confidence: 99%

Review: Microbial analysis in dielectrophoretic microfluidic systems

Fernandez

Rohani

Farmehini

et al. 2017

Analytica Chimica Acta

122

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Infections caused by various known and emerging pathogenic microorganisms, including antibiotic-resistant strains, are a major threat to global health and well-being. This highlights the urgent need for detection systems for microbial identification, quantification and characterization towards assessing infections, prescribing therapies and understanding the dynamic cellular modifications. Current state-of-the-art microbial detection systems exhibit a trade-off between sensitivity and assay time, which could be alleviated by selective and label-free microbial capture onto the sensor surface from dilute samples. AC electrokinetic methods, such as dielectrophoresis, enable frequency-selective capture of viable microbial cells and spores due to polarization based on their distinguishing size, shape and sub-cellular compositional characteristics, for downstream coupling to various detection modalities. Following elucidation of the polarization mechanisms that distinguish bacterial cells from each other, as well as from mammalian cells, this review compares the microfluidic platforms for dielectrophoretic manipulation of microbials and their coupling to various detection modalities, including immuno-capture, impedance measurement, Raman spectroscopy and nucleic acid amplification methods, as well as for phenotypic assessment of microbial viability and antibiotic susceptibility. Based on the urgent need within point-of-care diagnostics towards reducing assay times and enhancing capture of the target organism, as well as the emerging interest in isolating intact microbials based on their phenotype and subcellular features, we envision widespread adoption of these label-free and selective electrokinetic techniques.

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Section: Introductionmentioning

confidence: 99%

Review: Microbial analysis in dielectrophoretic microfluidic systems

Fernandez

Rohani

Farmehini

et al. 2017

Analytica Chimica Acta

122

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“…As demonstrated in the recent reviews on capillary and microchip ITP , applications of this CE mode are much more frequently oriented to determination of small ions, e.g. anionic counterions of basic peptides than to analyses of peptides themselves.…”

Section: Separations In Different Ce and Cec Modesmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2013–middle 2015)

Kašička

2015

Electrophoresis

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The review brings a comprehensive survey of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, micropreparation, purification, and physicochemical and biochemical characterization of peptides in the years 2013, 2014, and ca. up to the middle of 2015. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are described. New developments in particular CE and CEC modes are presented and several types of their applications to peptide analysis are reported: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence, and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

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“…As follows from the reviews on the recent progresses and applications of CITP and microchip ITP , direct applications of this mode to analytical and preparative separations of peptides are relatively limited. However, ITP or t‐ITP are often employed as preconcentration and/or preseparation steps prior to CE analysis of cationic or anionic analytes including peptides present at low concentrations and/or in complex mixtures.…”

Section: Separations In Different Ce and Cec Modesmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2011–2013)

Kašička

2013

Electrophoresis

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The review presents a comprehensive survey of recent developments and applications of capillary and microchip electroseparation methods (zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography) for analysis, isolation, purification, and physicochemical and biochemical characterization of peptides. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are presented. New developments in particular CE and CEC modes are reported and several types of their applications to peptide analysis are described: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide relevant physicochemical characteristics of peptides are demonstrated.

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Microfluidic isotachophoresis: A review

Cited by 75 publications

References 152 publications

Review: Microbial analysis in dielectrophoretic microfluidic systems

Review: Microbial analysis in dielectrophoretic microfluidic systems

Recent developments in capillary and microchip electroseparations of peptides (2013–middle 2015)

Recent developments in capillary and microchip electroseparations of peptides (2011–2013)

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