Microfluidic immunoaffinity separations for bioanalysis

Analysis of free and bound NADPH in aqueous extract of human placenta used as wound healer

“…Developed profile was similar to a typical immuno-affinity chromatogram [33]. It shows that 75.1 ± 2.2% of the total fluorescence emission (ex: 340 nm; em: 450 nm) was eluted as unabsorbed fraction while the rest was co-eluted with the peptides (Fig.…”

Analysis of free and bound NADPH in aqueous extract of human placenta used as wound healer

“…Developed profile was similar to a typical immuno-affinity chromatogram [33]. It shows that 75.1 ± 2.2% of the total fluorescence emission (ex: 340 nm; em: 450 nm) was eluted as unabsorbed fraction while the rest was co-eluted with the peptides (Fig.…”

Development of Immunoaffinity Restricted Access Media for Rapid Extractions of Low-Mass Analytes

“…This difference versus the result for the Schiff base method may also reflect the more ordered orientation of antibodies in the hydrazide method, which could have made more F ab regions accessible to papain for cleavage. It should be noted that larger differences between these two immobilization methods may be observed for some types of antibodies and, in particular, monoclonal antibodies that have a relatively low degree glycosylation compared to typical polyclonal antibodies 38,39. However, this issue was not found to be a problem for the monoclonal anti-fluorescein antibodies that were used later in this current study.…”

Investigation of Antigen-Antibody Interactions of Sulfonamides with a Monoclonal Antibody in a Fluorescence Polarization Immunoassay Using 3D-QSAR Models

“…The antigen-antibody complexes form as a result of several intermolecular forces; the five main forces are: (a) hydrogen-bonding; (b) coulombic, i.e. , electrostatic and dipole-dipole forces; (c) van der Waals; (d) hydrophobic interactions; and (e) π– π complementary ring-bonding [ 23 ]. Since adhesion forces between a small molecule and a protein are mainly non-covalent, the forces governing antigen-antibody binding are similar to that of drug-receptor binding.…”

Analysis of free and bound NADPH in aqueous extract of human placenta used as wound healer

“…Developed profile was similar to a typical immuno-affinity chromatogram [33]. It shows that 75.1 ± 2.2% of the total fluorescence emission (ex: 340 nm; em: 450 nm) was eluted as unabsorbed fraction while the rest was co-eluted with the peptides (Fig.…”

Development of Immunoaffinity Restricted Access Media for Rapid Extractions of Low-Mass Analytes

“…This difference versus the result for the Schiff base method may also reflect the more ordered orientation of antibodies in the hydrazide method, which could have made more F ab regions accessible to papain for cleavage. It should be noted that larger differences between these two immobilization methods may be observed for some types of antibodies and, in particular, monoclonal antibodies that have a relatively low degree glycosylation compared to typical polyclonal antibodies 38,39. However, this issue was not found to be a problem for the monoclonal anti-fluorescein antibodies that were used later in this current study.…”

Investigation of Antigen-Antibody Interactions of Sulfonamides with a Monoclonal Antibody in a Fluorescence Polarization Immunoassay Using 3D-QSAR Models

“…The antigen-antibody complexes form as a result of several intermolecular forces; the five main forces are: (a) hydrogen-bonding; (b) coulombic, i.e. , electrostatic and dipole-dipole forces; (c) van der Waals; (d) hydrophobic interactions; and (e) π– π complementary ring-bonding [ 23 ]. Since adhesion forces between a small molecule and a protein are mainly non-covalent, the forces governing antigen-antibody binding are similar to that of drug-receptor binding.…”

Analysis of free and bound NADPH in aqueous extract of human placenta used as wound healer

“…Developed profile was similar to a typical immuno-affinity chromatogram [33]. It shows that 75.1 ± 2.2% of the total fluorescence emission (ex: 340 nm; em: 450 nm) was eluted as unabsorbed fraction while the rest was co-eluted with the peptides (Fig.…”

Development of Immunoaffinity Restricted Access Media for Rapid Extractions of Low-Mass Analytes

“…This difference versus the result for the Schiff base method may also reflect the more ordered orientation of antibodies in the hydrazide method, which could have made more F ab regions accessible to papain for cleavage. It should be noted that larger differences between these two immobilization methods may be observed for some types of antibodies and, in particular, monoclonal antibodies that have a relatively low degree glycosylation compared to typical polyclonal antibodies 38,39. However, this issue was not found to be a problem for the monoclonal anti-fluorescein antibodies that were used later in this current study.…”

Investigation of Antigen-Antibody Interactions of Sulfonamides with a Monoclonal Antibody in a Fluorescence Polarization Immunoassay Using 3D-QSAR Models

“…The antigen-antibody complexes form as a result of several intermolecular forces; the five main forces are: (a) hydrogen-bonding; (b) coulombic, i.e. , electrostatic and dipole-dipole forces; (c) van der Waals; (d) hydrophobic interactions; and (e) π– π complementary ring-bonding [ 23 ]. Since adhesion forces between a small molecule and a protein are mainly non-covalent, the forces governing antigen-antibody binding are similar to that of drug-receptor binding.…”