Insect damage in canola adversely affects its productivity andquality and is considered one of the most important degrading factors in Egypt. The effect of foliar application of salicylic acid (SA) on aphid populations, growth and yield of canola (Brassica napus, L.) cv. serw 4 was the major goal of this study. Two experiments were conducted at the farm of Faculty of Agriculture, Suez Canal University, Ismailia, Egypt, during 2014 and 2015 seasons, to achieve this target. Each experiment included four levels of SA (0, 50, 100, 200 mg 1-1). The experimental results revealed that SA, at low concentration (50 mg 1-1), was an effective treatment for reduction the number of aphid populations and colony depth on the main inflorescence, contributed with reducing the thickness of secretory tissue of flower pedicel. The level of 50 mg 1-1 of SA-treated canola had the highest number of stomata cm-2, along with the lowest width of both stoma and its aperture. Thickness of xylem tissue and the number of xylem vessels bundle-1 in leaf midrib, reducing sugars and free amino acids was increased at 50 mg 1-1 SA, but free phenolics content did not affected significantly. Under controlled conditions, changes in temperature of infected leaves allowed the discrimination between healthy and infected areas in thermo-image, even before visible symptoms of aphid infestation appeared. The detection of modifications in plants or canopies, associated with low insect severity in the early stages of infestation, was crucial for the targeted, site-specific or on demand application of integrated aphid control. Canola, which was treated with 50 mg 1-1 of SA, gave 30.5 and 27.9 kg of oil ha-1 over the control. It was concluded that spraying of SA at 50 mg 1-1 was an effective elicitor to diminish the aphid numbers on canola inflorescence and improve its yield.
Mahmoud M.F., Barta M., 2011. Effect of gamma radiation on the male sterility and other quality parameters of peach fruit fly, Bactrocera zonata (Saunders) (Diptera: Tephritidae). Hort. Sci. (Prague), 38: 54-62.Bactrocera zonata, a serious pest of fruits in many parts of the world, has recently been recorded in Northern Africa. Even though it has not been introduced to the European continent yet, a strong emphasis is being placed on developing effective measures to suppress this pest and to prevent it from establishing in neighbouring European countries. The sterile insect technique is widely used in integrated programmes against tephritid fruit flies and, in this paper, quality parameters of irradiated B. zonata were evaluated for possible use of sterile insect technique within the management of this pest. Pupae were irradiated ( 60 Co) 48 h before adult emergence (in an air atmosphere) with doses of 10, 30, 50, 70 or 90 Gy. While adult emergence and egg hatch decreased with increasing dose, no significant differences in female fecundity were found among doses. Exposure of pupae to 90 Gy resulted in a total sterility of eggs laid by non-treated females crossed with treated males. Only insignificant difference in the radiation effect on female fecundity was found. Moderate effects on sex ratio and size were recorded, as they decreased gradually by increasing doses. No considerable effect on flying capability was observed, but generally, the percentage of fliers decreased with increasing radiation doses. Fried's competitiveness values of treated males (30 and 70 Gy) suggest that irradiated males compete successfully with non-irradiated ones.
The development of resistance to synthetic insecticides is one of the driving forces for changes in insect pest management. Governments regulatory bodies are in favour of environmentally safe chemicals with low toxicity, short-term persistence, and limited effects on non-target organisms as predominantly requirements for pesticides registration. Biological control can be considered as a powerful tool and one of the most important alternative control measure providing environmentally safe and sustainable plant protection. The success of biological control will depend on understanding the adaptation and establishment of applied biological control agents in agricultural ecosystems. Microbial pathogens and arthropod biocontrol agents, entomopathogenic nematodes (EPNs) have been successfully used in agricultural systems. They are highly virulent, killing their hosts quickly and can be cultured easilyin vivoorin vitro.They are safe for non-target vertebrates and for the environment, and production costs have been significantly reduced in recent times as they are mass produced in liquid media. Moreover, no difficulties to apply EPNs as they are easily sprayed using standard equipment and can be combined with almost all chemical control compounds. EPNs are widely used to control economically important insect pests in different farming systems: from fruit orchards, cranberry bogs and turf grass to nurseries and greenhouses. The use of EPNs for biocontrol began only in early 1980s and involved a step-by-step scientific and technical development. Mass production of the nematodes played a key role in the commercially development of insect pests control with nematodes.
ABSTRACT. The study was carried out at two experimental farms in Ismailia Governorate, Egypt, during season of 2015 and 2016. GF-120 (Conserve ® 0.024% CB) was selected in this study to evaluate its efficacy for controlling the peach fruit fly, Bactrocera zonata (Saunders) and the Mediterranean fruit fly, Ceratitis capitata (Wied.), on mango fruits by using partial bait spray and spots method. GF-120 was used as low environmental impact method and new way to control B. zonata and C. capitata, compared with malathion 57%, as traditional insecticide. During both seasons, data revealed that the number of C. capitata captured weekly from different treatments and untreated plots was higher than the number of B. zonata captured in both seasons of experiment. Data revealed that the number males of B. zonata and C. capitata captured weekly from sticky traps were lower in trees treated with GF-120 than malathion and untreated plots. In the farm of Faculty of Agriculture, Suez Canal University, the lowest percentages of infestation of both dropping and setting fruits per tree were recorded in plots treated with GF120 (spots), with an average of 25.14 and 17.022% for dropping fruits and 2.2 and 2.0% for setting fruits for 2015 and 2016 season, respectively. Data indicated that the all tested formulation under field condition caused significant reduction in the rate of infestation from 54.92 to 81.79 for both dropping and setting fruits. The lowest percentages of infestations in the private farm of both dropping and setting fruits per tree were recorded in GF-120 (spray treatment), with an average of 20.0, 12.12 for dropping fruits and 3.
In one-pot strategy, diazotization of methyl anthranilate 5 followed by addition of amino acid ester hydrochloride, we have prepared methyl-2-(4-oxobenzotriazin-3(4H)-yl)alkanoates 6a−c. Starting with hydrazides 7a,b, N-alkyl-2-(4-oxobenzotriazin-3(4H)-yl)alkanamides 9−10(a−h) and methyl-2-(2-(4-oxobenzotriazin-3(4H)-yl)alkanamido)alkanoates 11−12(a−e) were prepared via azide coupling. Hydrazones 13−15 were prepared via condensation of hydrazides 7a,b with 4-methoxybenzaldehyde, 4-dimethylaminobenzaldehyde, and/or arabinose. Molecular docking was done for synthesized compounds using MOE 2008-10 software. The compounds 9a, 12a, 12c, 13a, 13b, and 14b have the most pronounced strong binding affinities toward the target E. coli Fab-H receptor, whereas compounds 3, 11e, 12e, and 13a have the most pronounced strong binding affinities toward the target vitamin D receptor. The in vitro antibacterial activities of the highest binding affinity docked compounds were tested against E. coli, Staphylococcus aureus, and Salmonella spp. Majority of the tested compounds showed effective positive results against E. coli, while they were almost inactive against Staphylococcus aureus and Salmonella spp. The in vitro cytotoxic activities of the highest binding affinity-docked compounds were tested against the human liver carcinoma cell line (HepG2). Some compounds showed potent cytotoxic activity with low IC 50 values, especially for 3 (6.525 μM) and 13a (10.97 μM) than that for standard drug doxorubicin (2.06 μM).
A field experiment was conducted at the farm of Faculty of Agriculture, Suez Canal University, Ismailia Governorate, Egypt, during 2013/’14 and 2014/’15 seasons, to determine the effects of salicylic acid elicitor (SA) and potassium fertilizer (K) as foliar spray on canola production in the reclaimed land. Canola plants were sprayed with three rates of K and SA separately and together. The concentrations of SA with a surfactant triton 0.1% and concentrations of K sprayed after 30 days of sowing by one week interval for three times using hydraulic sprayer. Results indicated that K and SA provided good nutrition and resistance for pathogens, enhanced plant height (cm), number of branches/plant, fruiting zone (cm), seed yield/plant (g), seed yield/fed and oil percentage of canola cultivar (Serw 4) in the reclaimed land. K and SA separately or in combination at the rate of (6.0 cm−1 + 300 mg−1 SA) provided the best nutrition for enhancing resistance of plants against biotic and a biotic factors, consequently, enhancing vegetative growth and yield production during seasons of study 2013/’14 and 2014/’15.
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