Microfluidic Glass Chips with an Integrated Nanospray Emitter for Coupling to a Mass Spectrometer

Hoffmann, Peter; Häusig, Ulrich W.; Schulze, Philipp; Belder, Detlev

doi:10.1002/anie.200605152

Cited by 112 publications

(105 citation statements)

References 70 publications

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“…However, as UV-absorbance detection appears to be even more challenging in chip-based microfluidics, native fluorescence utilising UV excitation below 280 nm is, besides mass spectrometric detection [18][19][20], one of the most universal detection techniques in MCE. Deep UV intrinsic fluorescence enables the detection of various analytes ranging from small aromatics to large proteins.…”

Section: Introductionmentioning

confidence: 99%

Impact of laser excitation intensity on deep UV fluorescence detection in microchip electrophoresis

2008

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A high intensity 266 nm continuous wave (cw-) laser developed for material processing was utilised as an excitation source for sensitive native fluorescence detection of unlabelled compounds in MCE. This 120 mW laser was attached via an optical fibre into a commercial epifluorescence microscope. With this MCE set-up we evaluated the impact of laser power on the S/N of aromatic compounds as well as of proteins. Compared with a previous work which used a 4 mW pulsed laser for excitation, improved S/N for small aromatics and to a lesser extent for proteins could be attained. The LOD of the system was determined down to 24 ng/mL for serotonin (113 nM), 24 ng/mL for propranolol (81 nM), 80 ng/mL for tryptophan (392 nM) and 80 ng/mL for an aromatic diol (475 nM). Sensitive protein detection was obtained at concentrations of 5 mg/mL for lysocyme, trypsinogen and chymotrypsinogen (340, 208 and 195 nM, respectively). Finally, a comparison of the cw-with a pulsed 266 nm laser, operating at the same average power, showed a higher attainable sensitivity of the cw-laser. This can be attributed to fluorescence saturation and photobleaching effects of the pulsed laser at high pulse energies.

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Section: Introductionmentioning

confidence: 99%

Impact of laser excitation intensity on deep UV fluorescence detection in microchip electrophoresis

2008

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“…Moreover, electrospray ionization mass spectrometry (ESI-MS) has been successfully integrated with microfluidic formats in an on-line fashion. [24][25][26][27] However, its integration with microdroplet microfluidics has remained a challenge. [28] The direct MS analysis of microdroplets is problematic for several reasons.…”

mentioning

confidence: 99%

Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online

Fidalgo¹,

Whyte²,

Ruotolo³

et al. 2009

Angew Chem Int Ed

165

128

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Fully integrated: Mass spectrometry has been integrated into a detection scheme for microdroplets that are created within microfluidic channels (see picture, scale bar 200 microm). This technique allows droplets to be identified based on the compounds they contain, and combines fluorescence screening with MS analysis. These experiments indicate how similar approaches can be applied to the ambitious goals of on-chip protein evolution and chemical synthesis.

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“…Koster et al [14] conducted a thorough summary of the materials used for microfluidic chips. The fabrication process of integrated nozzles on microfluidic chips was generally easier with polymers than with glass [34,35]. Among these polymers, PDMS was a widely used material for microfluidic chips because of its chemical inertness, low cost, and rapid fabrication process by soft lithography [36].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Electro-Sonic Flow Focusing Ionization Microfluidic Chip for Mass Spectrometry

Qian

Chen

et al. 2015

Micromachines

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Increasing research efforts have been recently devoted to the coupling of microfluidic chip-integrated ionization sources to mass spectrometry (MS). Considering the limitations of microfluidic chips coupled with MS such as liquid spreading, dead volume, and manufacturing troubles, this paper proposed a new three-dimensional (3D) flow focusing (FF)-based microfluidic ionizing source. This source was fabricated by using the two-layer soft lithography method with the nozzle placed inside the chip. The proposed FF microfluidic chip can realize two-phase FF with liquid in air regardless of the viscosity ratio of the continuous and dispersed phases. MS results indicated that the proposed FF microfluidic chip can work as a typical electrical ionization source when supplied with high voltage and can serve as a sonic ionization source without high voltage. The electro-sonic FF ionization microfluidic chip is expected to have various applications, particularly in the integrated and portable applications of ionization sources coupling with portable MS in the future.

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Microfluidic Glass Chips with an Integrated Nanospray Emitter for Coupling to a Mass Spectrometer

Cited by 112 publications

References 70 publications

Impact of laser excitation intensity on deep UV fluorescence detection in microchip electrophoresis

Impact of laser excitation intensity on deep UV fluorescence detection in microchip electrophoresis

Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online

Three-Dimensional Electro-Sonic Flow Focusing Ionization Microfluidic Chip for Mass Spectrometry

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